The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature

Li, Feiyun; Qin, Feiyang; Pang, Yongqiang; Li, Hongming; Cai, Cheng; Liu, Weifeng; Qian, Yong; Qiu, Xueqing

doi:10.1039/d0gc04333h

Cited by 13 publications

(39 citation statements)

References 24 publications

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“…This is attributed to the reduced molecular hydrophilicity for ionic associations and attracted hydrophobic molecular chain; when the temperature is about UCST, due to increased temperature destroying the associations, the molecular chain is hydrated and stretched, and the polymer will be re-dissolved. 15,16,20 As predicted, PPSP has a sensitive UCST response, as shown in Figure 3.…”

Section: ■ Introductionsupporting

confidence: 73%

“…CCR was pretreated with autoclave at 120 °C for 1 h, and the contents of glucan and acid-insoluble lignin of CCR were 87.5 and 7.6%, respectively. 15 CTec2 was purchased from Novozymes Group (Shanghai, China), with a protein content of 73.6 mg/mL and enzyme activity of 147 FPU/mL. The enzyme activity was determined according to the method described in the previous literature.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The enzyme activity was determined according to the method described in the previous literature. 15 All other chemical reagents in the experiment were analytically pure, and all solutions were prepared with acetic acid−sodium acetate buffer solution (pH = 5.0, 50 mM).…”

Section: ■ Introductionmentioning

confidence: 99%

“…In addition, when the concentration ratio of PPSP 10 -3 to cellulase was unchanged, the cellulase recovery rate increased with the increased cellulase concentration. 15 For 0.2 g/L CTec2 solution, PSPE could be used to recover about 50% of cellulase. 15 PPSP could only be used to recover about 25% of cellulase.…”

Section: ■ Introductionmentioning

confidence: 99%

“…15 For 0.2 g/L CTec2 solution, PSPE could be used to recover about 50% of cellulase. 15 PPSP could only be used to recover about 25% of cellulase. This is due to the introduction of TPEG in PPSP, which increased the hydrophilicity of the polymer.…”

Section: ■ Introductionmentioning

confidence: 99%

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Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Zhang

et al. 2023

ACS Sustainable Chem. Eng.

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The cost of lignocellulosic enzymatic hydrolysis can be reduced by adding additives with upper critical solution temperature (UCST) response. A recyclable UCST-type copolymer PPSP was synthesized, for enhancing lignocellulosic enzymatic hydrolysis and the cellulase recovery effect of substrate reabsorption. For the hydrolysis system of corncob residue without adding PPSP, only 50% of cellulase amount was saved at a room temperature of 25 °C by adding the fresh corncob residue. The substrate enzymatic digestion efficiency for 72 h was increased by 1.3 times with only adding 0.5 g/L PPSP 10 -3. After the hydrolysis, 70% of cellulase amount was saved at 25 °C by using the above method. Both lignocellulosic hydrolysis and recovery of cellulase performances were improved by adding PPSP. This may be attributed to the fact that PPSP preferentially adsorbed on lignin to form a hydration membrane to reduce the no-productive adsorption of cellulase and enhanced lignocellulosic enzymatic hydrolysis, while increasing the concentration of free cellulase in the system and strengthening recovery of cellulase. This work will provide one way to reduce the cost of lignocellulosic enzymatic hydrolysis.

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Section: ■ Introductionsupporting

confidence: 73%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Zhang

et al. 2023

ACS Sustainable Chem. Eng.

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Rational design of temperature‐responsive binary polymers containing sulfobetaine and quaternary ammonium fragments for application in lignocellulosic hydrolysis

Pan,

Li,

Yuan

et al. 2024

J of Applied Polymer Sci

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Enzymatic strengthening and cellulase recovery are effective ways for improving the economy of lignocellulosic hydrolysis. A series of temperature‐responsive quaternary ammonium salt type enzymatic hydrolysis promoters (PSDM) were obtained by aqueous‐solution free radical polymerization. PSDM dissolved under lignocellulosic enzymatic hydrolysis conditions (50°C). After hydrolysis, it co‐precipitated with cellulase when cooling. PSDM had significantly improved hydrolysis and recycling enzyme properties. The efficiency of high‐solid substrate enzymatic hydrolysis of corncob residues improved to 1.2 times, and 50% of the cellulase amount was saved when adding 1.5 g/L of PSDM1 to the system. Hydrophobic binding may be the main driving force for co‐precipitation between PSDM and cellulase. In this work, regulation of glucose yield and cellulase consumption were realized by adjusting the ratio of positive and negative charges in PSDM. This work provides a new idea for reducing the cost of technology for this lignocellulosic sugar platform.

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Synthesis of temperature and pH responsive lignin-grafted sulfobetaine for efficiently recycling cellulase

Pang²,

Li³

et al. 2023

Bioresource Technology

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The synthesis of a UCST-type zwitterionic polymer for the efficient recycling of cellulase at room temperature

Abstract: In order to reduce the enzyme cost for lignocellulosic enzymatic hydrolysis, the upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A...

Cited by 13 publications

References 24 publications

Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Synthesis of Recyclable UCST-Type Copolymer PPSP and Its Application in Enhancing Lignocellulosic Enzymatic Hydrolysis and Recycling Cellulase

Rational design of temperature‐responsive binary polymers containing sulfobetaine and quaternary ammonium fragments for application in lignocellulosic hydrolysis

Synthesis of temperature and pH responsive lignin-grafted sulfobetaine for efficiently recycling cellulase

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