The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture

The Phenotypic Complexity of Myogenic Clones

“…As shown in Fig. 1, the quantity and types of collagen subunits were indistinguishable from those synthesized by authentic fibroblasts cultured under the same conditions (10). The mononucleated cells not only synthesize the common collagen chains but they are assembled into typically striated collagen fibrils as observed by electron microscopy.…”

“…In all these secondary cultures there were large numbers of mononucleated cells that histologically were indistinguishable from authentic fibroblasts (10,23). To determine whether these cells had the biochemical properties of fibroblasts, the cultures were exposed to [3H]proline and analyzed for collagen as shown in Table 2.…”

“…/Ci/ml). The collagen subunits were analyzed as described in Schiltz et al (10). The kinds of glycosaminoglyeans synthesized in secondary monolayer cultures was followed with [3S sulfate (Amersham Searle, carrier-free sodium salt) or ['H ]glucosamine (New England Nuclear Corp., 3.6 Ci/mmol).…”

“…,ug/ml) and ,-aminoproprionitrile (125 ,ug/ml). After extraction, the culture was mixed with lathyritic chick-skin collagen and analyzed by chromatography on a carboxymethylcellulose column by the procedures described in Schiltz et al (10).…”

Effect of Extracellular Chondroitin Sulfate on Cultured Chondrocytes

“…Standard chondroitin sulfate was obtained from Miles Laboratories Inc., Elkhart, Ind. Collagen synthesis was monitored by pulse labeling with [~H]proline (10 Ci/mmol, 5 ,uCi/mL New England Nuclear) followed by proline/hydroxyproline determination by the methods of Lukens (1965) modified by Schiltz et al (1973).…”