The Synthesis of GoldMag Nano-Particles and their Application for Antibody Immobilization

Cui, Yali; Wang, Yani; Wang, Hui; Zhang, Zhifeng; Xin, Xiaofang; Chen, Chao

doi:10.1007/s10544-005-1596-x

Cited by 59 publications

(48 citation statements)

References 14 publications

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“…3,4 In contrast to the literature, the Fe 3 O 4 @Au composite MNPs in this present study had broader absorption spectra and redshifted maximum absorption wavelength. 3,38,39 Such a redshift phenomenon of the plasmon absorption can be understood in terms of plasmon hybridization with the gold shell layer growth. In addition, because the gold shell layer outside the Fe 3 O 4 core forms a rough surface after a coating of three iterations, strong interaction and coupling of the surface plasmon exists between neighboring nanoparticles, which may also contribute to the plasmon resonance redshift.…”

Section: Effect Of Fe 3 O 4 @Au Composite Mnps On the Organsmentioning

confidence: 99%

Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Li¹,

Liu²,

Zhong³

et al. 2011

IJN

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Purpose: This research was conducted to assess the biocompatibility of the core-shell Fe 3 O 4 @ Au composite magnetic nanoparticles (MNPs), which have potential application in tumor hyperthermia. Methods: Fe 3 O 4 @Au composite MNPs with core-shell structure were synthesized by reduction of Au 3+ in the presence of Fe 3 O 4 -MNPs prepared by improved co-precipitation. Cytotoxicity assay, hemolysis test, micronucleus (MN) assay, and detection of acute toxicity in mice and beagle dogs were then carried out. Results:The result of cytotoxicity assay showed that the toxicity grade of this material on mouse fibroblast cell line (L-929) was classified as grade 1, which belongs to no cytotoxicity. Hemolysis rates showed 0.278%, 0.232%, and 0.197%, far less than 5%, after treatment with different concentrations of Fe 3 O 4 @Au composite MNPs. In the MN assay, there was no significant difference in MN formation rates between the experimental groups and negative control (P . 0.05), but there was a significant difference between the experimental groups and the positive control (P , 0.05). The median lethal dose of the Fe 3 O 4 @Au composite MNPs after intraperitoneal administration in mice was 8.39 g/kg, and the 95% confidence interval was 6.58-10.72 g/kg, suggesting that these nanoparticles have a wide safety margin. Acute toxicity testing in beagle dogs also showed no significant difference in body weight between the treatment groups at 1, 2, 3, and 4 weeks after liver injection and no behavioral changes. Furthermore, blood parameters, autopsy, and histopathological studies in the experimental group showed no significant difference compared with the control group. Conclusion:The results indicate that Fe 3 O 4 @Au composite MNPs appear to be highly biocompatible and safe nanoparticles that are suitable for further application in tumor hyperthermia.

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Section: Effect Of Fe 3 O 4 @Au Composite Mnps On the Organsmentioning

confidence: 99%

Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Li¹,

Liu²,

Zhong³

et al. 2011

IJN

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“…4H 2 O solution in a beaker, and slowly mixed in a shaking incubator to allow adsorption of Au 3+ onto the magnetic particles. NH 2 OH solution is then added to the system while shaking the mixture for 1 h. Cui et al [254] uses this procedure to synthesis goldcoated Fe 3 O 4 NPs for antibody immobilization. The core was produced by co-precipitation of Fe (II) and Fe (III) ions in alkaline medium.…”

Section: Goldmentioning

confidence: 99%

Recent advances in surface engineering of superparamagnetic iron oxide nanoparticles for biomedical applications

Mahmoudi

Simchi

Imani

2010

JICS

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Superparamagnetic iron oxide nanoparticles (SPIONs) are promising materials for various biomedical applications including targeted drug delivery and imaging, hyperthermia, magneto-transfections, gene therapy, stem cell tracking, molecular/cellular tracking, magnetic separation technologies (e.g. rapid DNA sequencing), and detection of liver and lymph node metastases. The most recent applications for SPIONs for early detection of inflammatory, cancer, diabetes and atherosclerosis have also increased their popularity in academia. In order to increase the efficacy of SPIONs in the desired applications, especial surface coating/characteristics are required. The aim of this article is to review the surface properties of magnetic nanoparticles upon synthesis and the surface engineering by different coatings. The biological aspects, cytotoxicity, and health risks are addressed. Special emphasis is given to organic and inorganic-based coatings due to their determinant role in biocompatibility or toxicity of the final particles.

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“…GoldMag particles are particle complexes composed of magnetic particles as the core component with gold shell or gold nanoparticles chemically assembled on the magnetic core surface [1] . They offer advantages in both magnetic separation (using the magnetic particles) and the immobilization of biomolecules (through their gold shell or gold nanoparticles).…”

mentioning

confidence: 99%

“…The particles can be divided into two types: core/shell structure and assembled structure. The GoldMag particles with core/shell structures are prepared by reducing Au 3+ to Au 0 on the surface of Fe 3 O 4 nanoparticles [1,2] . GoldMag particles with assembled structure are formed in two steps.…”

mentioning

confidence: 99%

Synthesis of GoldMag particles with assembled structure and their applications in immunoassay

Cui

Zhang

et al. 2006

SCI CHINA SER B

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Micrometer-sized Fe 3 O 4 particles and nano-sized gold particles were first synthesized by methods of self-aggregation of surface-chemically modified Fe 3 O 4 nanoparticles and citrate reduction of the Au 3+ to Au 0 , respectively. Interaction between these two types of particles resulted in the assembly of nano-sized gold particles on the surface of the micrometer-sized Fe 3 O 4 particles, forming an assembled structure with the Fe 3 O 4 core particles around which are attached nano-sized gold particles. The Fe 3 O 4 /Au structure is named GoldMag particles with assembled structure. The synthetic process, structure, and magnetic property of the GoldMag particles were analyzed. GoldMag particles with assembled structure have an irregular shape, rough surface with a diameter of 2-3 μm. These particles exhibit the superparamagnetic property with saturated magnetization of 41 A·m 2 /kg. In a single step, antibodies could be readily immobilized onto the surface of the particles with a high binding capacity. The GoldMag particles can be used as a novel carrier in immunoassays. The maximum quantity of human IgG immobilized onto GoldMag particles was 330 μg/mg. In order to validate the quality of the GoldMag particles as immunoassay carriers, an immunoassay system was used. The relative amount of immobilized human IgG was measured by HRP-labeled anti human IgG. The coefficient of variation within parallel samples of each group was below 6% and the coefficient of variation of means between five groups carried out separately was below 7%. Based on the sandwich method, the Hepatitis B surface antigen (HBsAg) and interleukin-8 (IL-8) were also analyzed by qualitative and quantitative detection, respectively. The result indicated that the GoldMag particles with assembled structure were an ideal carrier in immunoassay.

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The Synthesis of GoldMag Nano-Particles and their Application for Antibody Immobilization

Cited by 59 publications

References 14 publications

Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Recent advances in surface engineering of superparamagnetic iron oxide nanoparticles for biomedical applications

Synthesis of GoldMag particles with assembled structure and their applications in immunoassay

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