The synthesis of reserve carbohydrate by yeast

McAnally, Rachel Anne; Smedley-Maclean, Ida

doi:10.1042/bj0310072

Cited by 14 publications

(5 citation statements)

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“…Most of this glycogen can be liberated by treatment with 0 5 N-acetic acid at 750 (Bell & Northcote, 1950); Dr Bell (personal communication) informs us that this relatively mild treatment was suggested by a similar effect of cold N-HCl reported by McAnally & Smedley-Maclean (1937). We have now found that after such treatment almost all the glucan becomes soluble in 3% (w/v) NaOH.…”

mentioning

confidence: 73%

The Location of Chitin in the Yeast Cell Wall

Bacon¹,

Davidson²,

Jones³

et al. 1966

Biochemical Journal

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confidence: 73%

The Location of Chitin in the Yeast Cell Wall

Bacon¹,

Davidson²,

Jones³

et al. 1966

Biochemical Journal

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“…If whole cells previously extracted with dilute aqueous alkali are treated with 0 5m-acetic acid at 80°the glycogen is extracted (Bell & Northeote, 1950), but with some difficulty (Peat, Whelan & Edwards, 1958 -Maclean, 1937;Hassid, Joslyn & McCready, 1941). The glycogen extracted by treatment with acetic acid does not appear to have suffered much degradation (Northcote, 1953b).…”

Section: Resultsmentioning

confidence: 99%

The glucan components of the cell wall of baker's yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure

Bacon¹,

Farmer²,

Jones³

et al. 1969

Biochemical Journal

169

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1. Commercial pressed baker's yeast, and cell walls prepared from it, were extracted in various ways and the products examined by a number of techniques, including infrared spectroscopy and electron microscopy. 2. The glucan components of the walls cannot be extracted from intact yeast cells by 3% (w/v) sodium hydroxide at 75 degrees , but at least one-third of the glucan of cell wall preparations is dissolved under these conditions, and more will dissolve after ultrasonic treatment. 3. If intact cells are given a preliminary treatment with acid the wall glucans dissolve in dilute aqueous alkali. 4. Acid conditions as mild as sodium acetate buffer, pH5.0, for 3hr. at 75 degrees are sufficient for this preliminary treatment; the glucan then dissolves in 3% sodium hydroxide at 75 degrees leaving a very small residue, which contains chitin and about 1% of the initial glucan of the wall. Dissolution is hindered by exclusion of air, or by a preliminary reduction with sodium borohydride, suggesting that some degradation of the glucan by alkali is taking place. 5. After treatment with 0.5m-acetic acid for 24hr. at 90 degrees the glucan dissolves slowly at room temperature in 3% sodium hydroxide, or in dimethyl sulphoxide. The extraction with acetic acid removes glycogen and a predominantly beta-(1-->6)-linked glucan (not hitherto recognized as a component of baker's yeast), but none of the beta-(1-->3)-glucan, which remains water-insoluble. 6. Without treatment with acid, the glucan is not significantly soluble in dimethyl sulphoxide, but can be induced to dissolve by ultrasonic treatment. 7. These results are interpreted by postulating the presence of an enclosing membrane, composed of chitin and glucan, that when intact acts as a semipermeable membrane preventing the escape of the alkali- and dimethyl sulphoxide-soluble fraction of the glucan. Mild acid treatments damage this membrane, and ultrasonic and ballistic disintegration disrupt it. 8. Some support for this hypothesis is given by the effects of certain enzyme preparations, which have been found to render a substantial part of the glucan extractable by dimethyl sulphoxide.

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“…Ling et al (1925) refer to two forms of glycogen, one ' a constituent ofthe plasma' and readily extractable with water from dried yeast, and the other 'more intimately connected with the cell wall'. McAnally & Smedley-Maclean (1937) showed that the residue after extraction with 60% potassium hydroxide contained magnesium and phosphate, and digestion ofthe residue with cold N-hydrochloric acidrendered water-soluble a polysaccharide resembling glycogen. Jeanloz (1944) purified yeast glycogen which was separated by electrodialysis into two fractions, one soluble, the remainder (73%) insoluble in water: some indication that yeast glycogen was partly phosphorylated was obtained.…”

Section: Discussionmentioning

confidence: 99%