The synthesis of serine and <i>Leuconostoc citrovorum</i> factor by cell suspensions of <i>Streptococcus faecalis</i> R

Lascelles, June; Woods, D. D.

doi:10.1042/bj0580486

Cited by 40 publications

(11 citation statements)

References 33 publications

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“…The qpthesis of citrovorum factor by suspensions of Leuconostoc mesenteroides P60 variant strain Lascelles & Woods (1954) showed that during the synthesis of serine from glycine and formate by suspensions of Streptococcus faecal& R, there was a concurrent formation of a factor or factors active for the growth of L. citrovorum. A similar appearance of this factor attended the production of serine by suspensions of L. mesedmoides P 60; glucose, formate and p-aminobenzoic acid were essential substrates ( Table 1; .…”

Section: Resultsmentioning

confidence: 99%

“…Carbon dioxide has the dual capacity of stimulating serine synthesis (Lascelles et al 1954;Cross, 1960) and of preventing leucovorin inactivation by Leuconostoc mesenteroides P 60. Any hypothesis about a relationship between these two activities would be supported if another compound which produced one of the phenomena also produced the other.…”

Section: The Replacement Of Cog By ~S C O T~j~c Acidmentioning

confidence: 99%

“…The test organism was S. faecalis Rogers (ATCC 8043) maintained as described by Lascelles & Woods (1954); the assay procedure was that of Lascelles & Woods (unpublished). Medium G of Lascelles & Woods (1954), modified by the exclusion of thymine and pteroylglutamic acid, and the addition of pyridoxine (1 mg./l.) was the basal medium.…”

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confidence: 99%

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The Synthesis of Citrovorum Factor by Leuconostoc mesenteroides P 60

CROSS

1960

Journal of General Microbiology

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SUMMARYWhen incubated with p-aminobenzoic acid, formate and glucose, suspensions of a v k a n t strain of ~o n o s t o c meseWoides strain P 60 formed two factors possessing folk acid activity. One of these factors behaved like rhizopterin in supporting the growth of Streptococczcs faecalis strain R but not of Lactobacillus cmei. The other, like leucovorin, supported the growth of Leucmstoc citrovmrn. The synthesis of 'citrovonun factor' was enhanced 5-to 20-fold by the presence of CO, in the gas phase. Sulphathiazole inhibited the synthesis of both factors, the inhibition being overcome competitively by p-aminobenzoic acid. Leucovorin was destroyed by suspensions of L. msenteroides P60 in phosphate-buffered glucose. The diminution of activity for L. citrovmm (decrease of citrovorum factor) was decreased when the incubation was carried out in a C0,-enriched atmosphere. Ascorbic acid, which behaved similarly to CO, in partially preventing inactivation of leucovorin by suspensions of L. me8Woides P60 variant strain, also increased the synthesis of citrovonun factor from formate and p-aminobenzoic acid and partially satisfied the requirement of the growing L. mesenteroides P60 for COB.

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Section: Resultsmentioning

confidence: 99%

Section: The Replacement Of Cog By ~S C O T~j~c Acidmentioning

confidence: 99%

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The Synthesis of Citrovorum Factor by Leuconostoc mesenteroides P 60

CROSS

1960

Journal of General Microbiology

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“…This corresponded to a 60-fold increase for strain Hfm growing in the absence of thiamin, but cultures of the other mutants increased by only seven-to tenfold during vitamin deprivation. Streptococcus faecalis was grown without aeration in medium G (Lascelles & Woods, 1954) modified by the addition of folic acid (0.1 mg/l), DL-IIPOIC acid (40pg/l) and either pyridoxal hydrochloride (0-2 mg/l) or DL-alanine (0.2 g/l) when vitamin €3,-deficient organisms were required. This organism was also grown in foIic acid assay medium (Difco) with added DL-lipoic acid (40 pgll) and either folic acid ( I mg11) or the following replacement mixture (mg/l) for preparing folate-deficient bacteria : thymine (50), adenine (I o), guanine ( IO), L-serine (ZOO), and L-methionine (200) according to Samuel, D'Asi & Rabinowitz (1970).…”

Section: E T H O D Smentioning

confidence: 99%

Studies on the Vitamin Nutrition of the Cellular Slime Mould Dictyostelium Discoideum

Watts¹,

Guest²

1975

Journal of General Microbiology

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S U M M A R YVitamin auxotrophs of Escherichia coli grown in the presence or absence of the corresponding vitamin were used as substrates for the growth of Dictyostelium discoideum strain Ax-2 in order to investigate some of the growth-factor requirements of this cellular slime mould. Compared with the growth yields observed on vitamin-sufficient auxotrophs and on a prototroph, the yields on lipoate-, folate-, thiamin-or biotin-depleted auxotrophs were low but could be restored by the addition of exogenous vitamin to the slime mould cultures. It is concluded that these four vitamins are essential nutrients for D. discoideurn. Similar experiments with other auxotrophs were inconclusive, indicating either that the slime mould does not require pantothenate, nicotinate or vitamin B, or that the strains of E. coli were insufficiently depleted to detect such requirements. It is also concluded that cobalamin is not an essential nutrient, since the myxamoebae grew well with E. cuEi which lacks this vitamin when grown in cobalamin-free media. Similar arguments suggest that ubiquinone and vitamin K are also non-essential.

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“…Teltrahydro,folic acid + CH2OH-CH (NH..) -COOH =CH2 (NH2) COOH + 5N10N, methylene tetrahydrofolic acid This enzyme has been highly ipunified from bacterial (7,19) and animal sources (12). Evidence for a similar reaction in higher plants was obtained from in vivo experiments in which radioactive glycine or compounds easily converted to glycine such as glycollate were infiltrated into plant sections and the formation of radioactive serine observed (9,16,17).…”

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confidence: 99%

Serine Transhydroxymethylase of Cauliflower (Brassica oleracea var. botrytis L.): Partial Purification and Properties

Mazelis

Liu

1967

Plant Physiol.

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Sutmnmiary. Serine transhydroxymethylase (EC 2.1.2.1) has been ,purified 46-fold from caulifloxver (Brassica oleracca var. botrytis L.). The enzyme was coifnpletely dependent on the presence of tetrahydrofol,ic acid for the conversion of serine to glycine.The addition of vpyridoxal phosphate gave a large increase in the reaction rate. A double pH optimum was observed with maxima at 7.5 and 9.5. The enzyTne is specific for i,-serine. The D-'isomer is neither a sulbstrate nor an inhibitor. The Michaelis'constants for L-serine, tetrahydrofolic acid, an,d pvridoxal phosphate were 300 /jM, 760 tr, and 24 AI, respectively. The addition of K'-also 'stimulated the reaction rate considerably. The effect was quite specific since all other metal ions tested either had very little, influence or were extremely inhibitory.

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The synthesis of serine and Leuconostoc citrovorum factor by cell suspensions of Streptococcus faecalis R

Abstract: The term folic acid will be used throughout in general reference to the group as a whole; individual members will be named specifically.

Cited by 40 publications

References 33 publications

The Synthesis of Citrovorum Factor by Leuconostoc mesenteroides P 60

The Synthesis of Citrovorum Factor by Leuconostoc mesenteroides P 60

Studies on the Vitamin Nutrition of the Cellular Slime Mould Dictyostelium Discoideum

Serine Transhydroxymethylase of Cauliflower (Brassica oleracea var. botrytis L.): Partial Purification and Properties

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