The synthesis of XTT: A new tetrazolium reagent that is bioreducible to a water‐soluble formazan

Abstract

A new tetrazolium salt, XTT, has been synthesized. XTT is reduced by a considerable variety of cell lines to a water‐soluble formazan. XTT appears to merit further investigation as a reagent for broader application to cell culture assay systems.

Potent and selective activity of a new carbocyclic nucleoside analog (Carbovir: NSC 614846) against human immunodeficiency virus In vitro

“…Data are derived from the MTA as previously described (18). An XTT-PMS solution is prepared immediately prior to its addition to the wells of the culture dish [i mg/ml XTT (19,20) solution in media without FCS]. The stock PMS (15.3 mg PMS/ml FBS) solution is diluted 1:100 (0.153 mg/ml).…”

Assessment of viability of microorganisms employing fluorescence techniques

“…The tetrazolium dye CTC has the unique advantage that the formazan product is fluorescent, which enormously enhances the sensitivity of the detection. CTC has been used to determine the number of respiring Micrococcus luteus, Listeria monocytogenes and Pseudomonas fluorescens in pure cultures (8,53,56,57), and respiring bacteria in water (18,106,111,119,129), soil (149), and biofilms (22,45,134,152,153 -tetrazolium}-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate (XTT) was synthesized, which has the special property that the formazan product is water-soluble (93). XTT was applied by Roslev and King (114) for determination of viable respiring bacteria, and by Tellier (137) for testing the susceptibility of yeast to various antibiotics, in colorimetric assays based on reduction of XTT.…”

A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line

“…The current pharmacopeias recommend the use of WST dye MTS for G-CSF bioassays. During the last two decades, a number of WST dyes have been developed for cell viability assays, including MTS and XTT (Paull et al, 1988), WST-1 (Ishiyama et al, 1993), WST-5 (Ishiyama et al, 1996), and WST-8 (Ishiyama et al, 1997). The WST-8 is more stable and sensitive than other WST dyes, and has multiple applications including testing of antimicrobials, anti-cancerous drug screening, bacterial and mammalian cell proliferation, cytotoxicity assays, etc.…”