The article proposes a calibration method based on array optical signal to effectively improve the measurement accuracy of a photoelectric testing system with intersecting detection areas. According to the measurement principle of the system, the proposed calibration theory is systematically described. The aim is to map the given results in the form of vector into the time sequence. Subsequently, we develop a calibration platform whose core components are an arbitrary waveform generator and a tunable laser, and thus it can generate a set of six optical signals with an exact sequential relationship. After receiving the optical signals, some measurement results with errors are obtained by the system. Many calibration operations is performed to obtain the systematic errors, which are the statistical average of the deviation between the given results and those with errors. The results show that the comprehensive performance of the proposed calibration method is superior to that of the traditional method. In addition, the proposed calibration method relative to the traditional ones has some advantages, such as high efficiency, safety and low cost.