The systemic inflammatory response syndrome

Meeran, Hanif; Messent, Mark

doi:10.1177/146040860100300203

Cited by 10 publications

(22 citation statements)

References 75 publications

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“…Age-, sex-, and weight-matched WT and P2X 1 2/2 mice were injected i.p. with the specified dose of LPS in normal saline.…”

Section: Mouse Model Of Endotoxemiamentioning

confidence: 99%

“…The lung and liver tissues of WT and P2X 1 2/2 mice were removed and fixed in 4% paraformaldehyde overnight. The fixed samples were washed in PBS, snap-frozen in OCT embedding medium, and cross sectioned (5 mm thick).…”

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

“…Intravital microscopy of mouse cremaster muscle postcapillary venules WT and P2X 1 2/2 male mice, transiently anesthetized with inhaled isoflurane, were injected intrascrotally (i.s.) with LPS (10 ng, 400 ml) or vehicle (saline).…”

Section: Isolation Of Mouse Bone Marrow Neutrophils and Lps Treatmentmentioning

confidence: 99%

“…Neutrophils isolated from the bone marrow of WT or P2X 1 2/2 male mice were labeled with DDAO-SE (5 mM, red) or carboxy-DFFDA, SE (5 mM, green), respectively, at 37˚C for 15 min. The labeled cells (4 3 10 6 neutrophils in HBSS containing 0.5 U/ml apyrase) were mixed (1:1) and injected i.v.…”

Section: Adoptive Transfer Of Neutrophilsmentioning

confidence: 99%

“…SIRS may occur as a consequence of various pathogenic events, with sepsis syndrome being the most prevalent and lethal cause (1).…”

mentioning

confidence: 99%

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The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice

Maître

Magnenat

Heim

et al. 2015

The Journal of Immunology

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Extracellular ATP is becoming increasingly recognized as an important regulator of inflammation. However, the known repertoire of P2 receptor subtypes responsible for the proinflammatory effects of ATP is sparse. We looked at whether the P2X1 receptor, an ATP-gated cation channel present on platelets, neutrophils, and macrophages, participates in the acute systemic inflammation provoked by LPS. Compared with wild-type (WT) mice, P2X1−/− mice displayed strongly diminished pathological responses, with dampened neutrophil accumulation in the lungs, less tissue damage, reduced activation of coagulation, and resistance to LPS-induced death. P2X1 receptor deficiency also was associated with a marked reduction in plasma levels of the main proinflammatory cytokines and chemokines induced by LPS. Interestingly, macrophages and neutrophils isolated from WT and P2X1−/− mice produced similar levels of proinflammatory cytokines when stimulated with LPS in vitro. Intravital microscopy revealed a defect in LPS-induced neutrophil emigration from cremaster venules into the tissues of P2X1−/− mice. Using adoptive transfer of immunofluorescently labeled neutrophils from WT and P2X1−/− mice into WT mice, we demonstrate that the absence of the P2X1 receptor on neutrophils was responsible for this defect. This study reveals a major role for the P2X1 receptor in LPS-induced lethal endotoxemia through its critical involvement in neutrophil emigration from venules.

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“…Age-, sex-, and weight-matched WT and P2X 1 2/2 mice were injected i.p. with the specified dose of LPS in normal saline.…”

Section: Mouse Model Of Endotoxemiamentioning

confidence: 99%

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

Section: Isolation Of Mouse Bone Marrow Neutrophils and Lps Treatmentmentioning

confidence: 99%

Section: Adoptive Transfer Of Neutrophilsmentioning

confidence: 99%

“…SIRS may occur as a consequence of various pathogenic events, with sepsis syndrome being the most prevalent and lethal cause (1).…”

mentioning

confidence: 99%

See 3 more Smart Citations

The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice

Maître

Magnenat

Heim

et al. 2015

The Journal of Immunology

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Trauma-related sepsis and multiple organ failure: Current concepts in the diagnosis and management

Bumbaširević¹,

Karamarković²,

Lešić³

et al. 2005

Current Orthopaedics

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Early Internal Fracture Fixation Prevents Bacterial Translocation

Öztuna

Ersöz²,

Ayan³

et al. 2006

Clinical Orthopaedics and Related Research

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The aim of our study was to determine whether early internal fixation of major bone fractures helps prevent bacterial translocation in patients with multitrauma. Thirty-seven Sprague-Dawley rats were divided into three groups: (1) anesthesia only (n = 12); (2) the trauma group: tibia and femur fractures and moderate head trauma under anesthesia (n = 14); and (3) the fixation group: fixation of tibia and femur fractures and moderate head trauma under anesthesia (n = 11). After 24 hours, mesenteric lymph nodes, liver, spleen, and systemic blood samples were quantitatively cultured. The terminal ileum was assessed histopathologically. The incidence of bacterial translocation was less in the anesthesia group (two of 12 rats) and the fixation group (two of 11 rats) than in the trauma group (10 of 14 rats). The number of organs containing viable bacteria was significantly lower in the fixation group than in the trauma group. Histopathologically, villous architecture was preserved mostly in the fixation group; however, marked mucosal damage was detected in the trauma group. Our data suggest early internal fixation of long bone fractures in polytraumatized experimental animals with head injury results in preservation of the intestinal mucosal barrier and decreased bacterial translocation from the gut.

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The systemic inflammatory response syndrome

Cited by 10 publications

References 75 publications

The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice

The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice

Trauma-related sepsis and multiple organ failure: Current concepts in the diagnosis and management

Early Internal Fracture Fixation Prevents Bacterial Translocation

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