2007
DOI: 10.1074/jbc.m608153200
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The T4 Phage UvsW Protein Contains Both DNA Unwinding and Strand Annealing Activities

Abstract: UvsW protein belongs to the SF2 helicase family and is one of three helicases found in T4 phage. UvsW governs the transition from origin-dependent to origin-independent replication through the dissociation of R-loops located at the T4 origins of replication. Additionally, in vivo evidence indicates that UvsW plays a role in recombination-dependent replication and/or DNA repair. Here, the biochemical properties of UvsW helicase are described. UvsW is a 3 to 5 helicase that unwinds a wide variety of substrates, … Show more

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Cited by 29 publications
(67 citation statements)
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“…2). We did not detect significant unwinding of double-stranded substrates with either 5Ј or 3Ј single-stranded overhangs (the latter contrary to an earlier report (18)), or double-stranded DNA with a flayed single-stranded end (Fig. 2).…”
Section: Cloning and Expression Of Uvsw-uvsw And Uvsw-k141rcontrasting
confidence: 99%
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“…2). We did not detect significant unwinding of double-stranded substrates with either 5Ј or 3Ј single-stranded overhangs (the latter contrary to an earlier report (18)), or double-stranded DNA with a flayed single-stranded end (Fig. 2).…”
Section: Cloning and Expression Of Uvsw-uvsw And Uvsw-k141rcontrasting
confidence: 99%
“…Subsequent x-ray crystallography of the N-terminal region of UvsW-K141R confirmed that the protein fits the structural profile of a superfamily II DNA helicase and suggested unique structural domains for DNA binding (17). UvsW has also been shown to function as a 3Ј 3 5Ј helicase and to promote single-stranded DNA annealing (18).…”
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confidence: 80%
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“…Based on recent results from S. cerevisiae and P. furiosus, which demonstrated the involvement of helicases in MR-dependent DSB resection (42, 59), a possible candidate is UvsW helicase. UvsW helicase is known to be necessary for DSB repair (67,68) and is a functional analog of Sgs1, which is the S. cerevisiae helicase involved in DSB resection. We are currently in the process of testing the above hypothesis.…”
mentioning
confidence: 99%