The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

Verhertbruggen, Yves; Bouder, Axelle; Vigouroux, Jacqueline; Alvarado, Camille; Geairon, Audrey; Guillon, Fabienne; Wilkinson, Mark; Stritt, Fabian; Pauly, Markus; Lee, Mi Yeon; Mortimer, Jenny C.; Scheller, Henrik Vibe; Mitchell, Rachel; Voiniciuc, Cătălin; Saulnier, Luc; Chateigner‐Boutin, Anne‐Laure

doi:10.1016/j.plantsci.2020.110693

Cited by 12 publications

(14 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While ivory nut mannan was digested to small manno-oligosaccharides (DP ≤ 4), konjac glucomannan showed additional peaks with a retention time above the mannohexaose (DP 6) standard. These larger peaks, known to be diagnostic of glucomannan [15], represented only 1% of ivory nut oligosaccharide area but 48% of konjac sample area (Fig. 4c).…”

Section: Resultsmentioning

confidence: 99%

“…While ivory nut mannan (INM) was digested to small manno-oligosaccharides (degree of polymerization, DP ≤ 4), konjac glucomannan (KGM) showed additional peaks with a retention time above the mannohexaose (DP 6) standard. These larger peaks, known to be diagnostic of glucomannan (Verhertbruggen et al, 2021), represented only 1% of INM oligosaccharide area but 48% of KGM area (Figure 4c). Even when increasing the starting amount of INM, the digestion profiles did not resemble the glucomannan peaks of KGM (Figure S4).…”

Section: Influence Of Top Domain Swaps On (Gluco)mannan Productionmentioning

confidence: 99%

“…(Gluco)mannans can be decorated with galactose side chains (Voiniciuc et al, 2015;Yu et al, 2018) or O-acetylated (Zhong et al, 2018;Zhong et al, 2019), which increases their solubility (Berglund et al, 2020). HM is abundant in the endosperms of most legume seeds (Bewley et al, 2013), and to a lesser extent in cereal grains (Verhertbruggen et al, 2021). Plant HM polymers confer important physiological properties, such as stabilizing extracellular matrices, which are also commercially valuable (Bewley et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…(Gluco)mannans can be decorated with galactose side chains [9,10] or O -acetylated [11,12], which increases their solubility [13]. HM is abundant in the endosperms of most legume seeds [14], and to a lesser extent in cereal grains [15], which confer important physiological properties, such as stabilizing the extracellular matrix. Since HMs are excellent thickening and gelling agents, locust bean gum (extracted from Ceratonia siliqua , also known as carob) and guar gum (from Cyamopsis tetragonoloba ) are commonly used hydrocolloids in the food (approved as E410 and E412 stabilizers within the European Union), cosmetic and pharmaceutical industries.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Robert

Waldhauer

Stritt

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Interest in the engineering of polysaccharide-based biomaterials has emerged in recent years. Despite impressive advances on bacterial cellulose, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes. Here, we optimized the cultivation of the yeast Pichia pastoris for the orthogonal production of plant polysaccharides, and enhanced heteromannan (HM) production by assembling modular cellulose synthase-like A (CSLA) enzymes. Chimeric proteins swapping the domains of a plant mannan synthase and a glucomannan synthase led, in three cases, to higher yields or improved growth compared to the parental CSLA enzymes. Prolonged expression of a glucomannan synthase from Amorphophallus konjac (AkCSLA3) was toxic to yeast cells, as demonstrated by reduced biomass accumulation and elevated uptake of dyes that are normally restricted to the extracellular matrix. However, no growth inhibition was observed for CSLA variants producing relatively pure mannan or a CSLC glucan synthase. The toxicity of AkCSLA3 was reduced by swapping its C-terminal region with that of a mannan synthase. HM production was further boosted by co-expressing chimeric CSLA proteins with the MANNAN-SYNTHESIS-RELATED1 (MSR1) putative glycosyltransferase. Interestingly, Pichia cells either increased or decreased in size depending on the CSLA variant expressed, and most of them remained viable even producing copious amounts of hemicellulose. Therefore, yeast modified with non-toxic plant polysaccharides could represent a modular chassis to produce and protect sensitive cargo such as therapeutic proteins.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Influence Of Top Domain Swaps On (Gluco)mannan Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Robert

Waldhauer

Stritt

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Growth of the wheat (Triticum aestivum L. cv Recital) plants and grain harvesting was carried out as described in [21]. Briefly, the plants were grown in a controlled glasshouse at INRAE Le Rheu (France), individual spikes were tagged at flowering and stages were assessed by the thermal time method (cumulated average temperature in Celsius degrees per day after flowering, °DAF).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan

et al. 2021

Self Cite

View full text Add to dashboard Cite

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

show abstract

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

View full text Add to dashboard Cite

The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

show abstract

The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

Cited by 12 publications

References 89 publications

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Contact Info

Product

Resources

About