The Tannase from red yeast
            <i>Rhodotorula glutinis</i>
            : purification and characterization

Ong, Chong-Boon; Demirtaş, İbrahim; Kassim, M. Jain

doi:10.1080/10242422.2022.2136523

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Optimum activity was observed at pH 6 with the value of 506 U/g/min tannase activities as shown in Figure 5. This finding corroborated with work reported by Chong-Boon et al, 15 who characterized tannase produced from red yeast, Rhodotorulla glutinis DB2. The authors explained that maximum tannase activity was observed at pH 6.0 with 100% activity, higher or lower than pH 6.0 conditions decreased the rate of enzyme reaction.…”

Section: Resultssupporting

confidence: 92%

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Detannification of Ananas comosus Rinds Juice with Tnnase Immobilized on the Beads of Chrysophyllum albidum Seeds

2024

TJPPS

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Billion tons of agro wastes that constitute hazard to the environment are generated annually all over the world. How to curb this menace remains a major challenge in the field of biotechnology. In this study, a novel immobilization material for microbial tannase was developed from Chrysophyllum albidum seeds. Optimization studies for effective performance of the novel immobilization material were conducted. The characterized immobilized tannase was explored for the detannification of tannin present in Ananas comosus rinds juice. Activities of free and immobilized tannase from R. mucilaginosa CBS 316 were 724.49 and 497.77 U/g/min respectively. Optimal temperature and pH for tannase activity were 35ºC and 7 respectively. Optimum tannase load, bead size and bead number for immobilized tannase were 35%, 8mm and 20 respectively. Immobilized tannase was reusable for up to 3 times. MgSO4, KCl, and ZnSO4 enhanced tannase activity while PbCl2, MgCl, BaCl2 and EDTA inhibited the activity of tannase. Tannin content of the three varieties of Ananas comosus rinds juice from Bene, Cotonou and Local source were drastically reduced from 17.8, 16.3 and 5.9 mg/100g to 3.9, 2.9 and 1.8 mg/100g respectively when treated with immobilized tannase. This study established the detannification of Ananas comosus rinds juice with microbial tannase immobilized on the novel beads of Chrysophyllum albidum seeds.

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Section: Resultssupporting

confidence: 92%

“…Effect of incubation pH was observed to be highest at pH 7 after which there was decreased tannase activity as shown in Figure 3. Tannase produced from Rhodotorulla species is maximal at pH 7.0 15 . Optimum temperature for tannase from R. mucilaginosa CBS 316 was 35℃ with 510.26 U/g/min tannase activities as shown in Figure 4.…”

Section: Resultsmentioning

confidence: 99%

Detannification of Ananas comosus Rinds Juice with Tnnase Immobilized on the Beads of Chrysophyllum albidum Seeds

2024

TJPPS

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Purification and structural analysis of tannase from novel bacteria of Bacillus cereus strain KMS3-1 isolated in marine sediment

Govindarajan,

Alrefaei,

Almutairi

et al. 2024

Food Bioscience

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Evaluation of antibacterial, antifungal and antibiofilm activities of A. baumannii-derived tannase and gallic acid against uropathogenic microorganisms

Abdulshaheed,

Hanafiah,

Nawaz

et al. 2024

Microbial Pathogenesis

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The Tannase from red yeast Rhodotorula glutinis : purification and characterization

Cited by 6 publications

References 26 publications

Detannification of Ananas comosus Rinds Juice with Tnnase Immobilized on the Beads of Chrysophyllum albidum Seeds

Detannification of Ananas comosus Rinds Juice with Tnnase Immobilized on the Beads of Chrysophyllum albidum Seeds

Purification and structural analysis of tannase from novel bacteria of Bacillus cereus strain KMS3-1 isolated in marine sediment

Evaluation of antibacterial, antifungal and antibiofilm activities of A. baumannii-derived tannase and gallic acid against uropathogenic microorganisms

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