The Tapetal Major Facilitator NPF2.8 Is Required for Accumulation of Flavonol Glycosides on the Pollen Surface in <i>Arabidopsis thaliana</i>

Grunewald, Stephan; Marillonnet, Sylvestre; Hause, Gerd; Haferkamp, Ilka; Neuhaus, H. Ekkehard; Veß, Astrid; Hollemann, Thomas; Vogt, Thomas

doi:10.1105/tpc.19.00801

Cited by 35 publications

(19 citation statements)

References 116 publications

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“…Especially flavonoids are substantially enriched during pollen development with highest abundance in mature pollen, there they act as ROS‐scavengers in heat stress response and enhance pollen tube growth (Muhlemann, Younts, & Muday, 2018; Paupiere et al, 2017). Various classes of flavonoids, which are exogenously produced by the tapetum and stored in pollen have been shown to play a role in pollen germination and tube growth (Grunewald et al, 2020; Wang et al, 2020) and generally serve as protection against UV‐irradiation (Li, Ou‐Lee, Raba, Amundson, & Last, 1993; Neugart et al, 2014). Besides flavonoids, polyamines are highly abundant secondary metabolites in pollen that directly attenuate ROS and promote antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 99%

Implications of ionizing radiation on pollen performance in comparison with diverse models of polar cell growth

Stephan

2020

Plant Cell & Environment

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Research concerning the effects of ionizing radiation (IR) on plant systems is essential for numerous aspects of human society, as for instance, in terms of agriculture and plant breeding, but additionally for elucidating consequences of radioactive contamination of the ecosphere. This comprehensive survey analyses effects of x‐ and γ‐irradiation on male gametophytes comprising primarily in vitro but also in vivo data of diverse plant species. The IR‐dose range for pollen performance was compiled and 50% inhibition doses (ID50) for germination and tube growth were comparatively related to physiological characteristics of the microgametophyte. Factors influencing IR‐susceptibility of mature pollen and polarized tube growth were evaluated, such as dose‐rate, environmental conditions, or species‐related variations. In addition, all available reports suggesting bio‐positive IR‐effects particularly on pollen performance were examined. Most importantly, for the first time influences of IR specifically on diverse phylogenetic models of polar cell growth were comparatively analysed, and thus demonstrated that the gametophytic system of pollen is extremely resistant to IR, more than plant sporophytes and especially much more than comparable animal cells. Beyond that, this study develops hypotheses regarding a molecular basis for the extreme IR‐resistance of the plant microgametophyte and highlights its unique rank among organismal systems.

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Section: Discussionmentioning

confidence: 99%

Implications of ionizing radiation on pollen performance in comparison with diverse models of polar cell growth

Stephan

2020

Plant Cell & Environment

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“…Flavonoids take part in male fertility in plants (Figure 2). The pollen wall is a durable structure that is crucial for plant reproduction and the exine of pollen grains is usually covered by flavonoid glycosides (Battat et al, 2019; Grunewald et al, 2020). In rice, the oschs1 mutant produces flavonoid‐depleted pollen grains and there is loss of male fertility (Wang et al, 2020a).…”

Section: Biofunctions Of Phenylpropanoid Metabolitesmentioning

confidence: 99%

“…In rice, the oschs1 mutant produces flavonoid‐depleted pollen grains and there is loss of male fertility (Wang et al, 2020a). Inhibiting the transport of flavonol‐3‐ O ‐sophorosides from the tapetum to the exine of pollen by disrupting the function of the flavonol sophoroside transporter (FST1) results in reduced pollen viability in A. thaliana (Grunewald et al, 2020). In rice, the development of intine, which is the inner layer of the pollen wall, also requires flavonoid metabolites, the production of which is regulated by OsUCL23, a member of the plant‐specific blue copper protein family of phytocyanins and the proton‐dependent oligopeptide transport (PTO) family of transporters (Zhang et al, 2020e).…”

Section: Biofunctions Of Phenylpropanoid Metabolitesmentioning

confidence: 99%

Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions

Dong

Lin

2021

JIPB

828

429

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Phenylpropanoid metabolism is one of the most important metabolisms in plants, yielding more than 8,000 metabolites contributing to plant development and plant-environment interplay. Phenylpropanoid metabolism materialized during the evolution of early freshwater algae that were initiating terrestrialization and land plants have evolved multiple branches of this pathway, which give rise to metabolites including lignin, flavonoids, lignans, phenylpropanoid esters, hydroxycinnamic acid amides, and sporopollenin. Recent studies have revealed that many factors participate in the regulation of phenylpropanoid metabolism, and modulate phenylpropanoid homeostasis when plants undergo successive developmental processes and are subjected to stressful environments. In this review, we summarize recent progress on elucidating the contribution of phenylpropanoid metabolism to the coordination of plant development and plantenvironment interaction, and metabolic flux redirection among diverse metabolic routes. In addition, our review focuses on the regulation of phenylpropanoid metabolism at the transcriptional, post-transcriptional, post-translational, and epigenetic levels, and in response to phytohormones and biotic and abiotic stresses.

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“…As previously presented in this review, the incorporation of phenolamides into the cell wall (Clarke and McCaig, 1982;Negrel et al, 1996;McLusky et al, 1999;King and Calhoun, 2005;Ishihara et al, 2008) and their localization on the surface of the pollen grain (Grienenberger et al, 2009, Delporte et al, 2018 imply their transport in the apoplast. Several membrane transporters involved in the release of parietal precursors from tapetum for the formation of sporopollenin have been characterized as the ABC transporters ABCG26 / ABCG3 for the polyketide (Quilichini et al, 2014;lefèvre et al, 2018) or the NPF transporter FST1 for the flavonol glycosides (Grunewald et al, 2020), but the tri-hydroxycinnamoyl spermidine transporters have not been identified. Multi-drug and toxic compound extrusion transporters (MATEs) are the best candidates for the transport of phenolamide across the membrane, as recently exemplified by the leaf export of p-coumaroylagmatine through DTX18 in Arabidopsis (Dobritzsch et al, 2016), but alternatively, the involvement of secretory vesicle systems could not be excluded (Xu et al, 2014).…”

Section: Phenolamide Glycosylation and Transportmentioning

confidence: 99%

Phenolamides in plants: an update on their function, regulation, and origin of their biosynthetic enzymes

Roumani

Besseau

Gagneul

et al. 2020

Journal of Experimental Botany

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Phenolamides represent a family of specialized metabolites, consisting of the association of hydroxycinnamic acid derivatives with aliphatic or aromatic amines. Since the discovery of the first phenolamide in the late 1940s, decades of phytochemical analyses have revealed a high structural diversity for this family and a wide distribution in the plant kingdom. The occurrence of structurally diverse phenolamides in almost all plant organs has led to early hypotheses on their involvement in floral initiation and fertility, as well as plant defense against biotic and abiotic stress. In the present work, we critically review the literature ascribing functional hypotheses to phenolamides and recent evidence on the control of their biosynthesis in response to biotic stress. We additionally provide a phylogenetic analysis of the numerous N-hydroxycinnamoyltransferases involved in the synthesis of phenolamides and discuss the potential role of other enzyme families in their diversification. The data presented suggest multiple evolutionary events that contributed to the extension of the taxonomic distribution and diversity of phenolamides.

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The Tapetal Major Facilitator NPF2.8 Is Required for Accumulation of Flavonol Glycosides on the Pollen Surface in Arabidopsis thaliana

Abstract: The tapetal major facilitator NPF2.8 is required for accumulation of flavonol glycosides on the pollen surface of Arabidopsis thaliana.

Cited by 35 publications

References 116 publications

Implications of ionizing radiation on pollen performance in comparison with diverse models of polar cell growth

Implications of ionizing radiation on pollen performance in comparison with diverse models of polar cell growth

Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions

Phenolamides in plants: an update on their function, regulation, and origin of their biosynthetic enzymes

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