Four new species of Mesobiotus (Tardigrada: Eutardigrada: Macrobiotidae) are described from Skåne County in the southernmost region of Sweden. All four species are distinguished morphologically and through differences in DNA sequences as supported by PTP and mPTP analyses. With the addition of Mesobiotus bockebodicus sp. nov., M. passim sp. nov., M. pseudoemiliae sp. nov., and M. zelmaesp. nov., the number of nominal species of Macrobiotidae within Sweden is increased to 26, 73% of which have been documented from Skåne. Finally, new morphological details and DNA sequences are presented for Mesobiotus emiliae,and the phylogenetic relationships within the genus is reconstructed using previously published and new 18S and COI gene sequences.