The Taspase1/Myosin1f-axis regulates filopodia dynamics

Hensel, Astrid; Stahl, Paul; Moews, Lisa; König, Lena; Patwardhan, Rutuja; Höing, Alexander; Schulze, Nina; Nalbant, Perihan; Stauber, Roland H.; Knauer, Shirley K.

doi:10.1016/j.isci.2022.104355

Cited by 5 publications

(8 citation statements)

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“…At the same time, Myo1e deficiency mainly affects activated B cells ( 24 ). In this regard, Taspase1 and Myo1f inversely correlate with differentiation in the stem cell-monocyte-macrophage lineage ( 33 ). Mechanistically, Myo1f is cleaved by the caspase 1 protease, which impaired its function, indicating a Tasp1 negative regulation of Myo1f during differentiation ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, Taspase1 and Myo1f inversely correlate with differentiation in the stem cell-monocyte-macrophage lineage ( 33 ). Mechanistically, Myo1f is cleaved by the caspase 1 protease, which impaired its function, indicating a Tasp1 negative regulation of Myo1f during differentiation ( 33 ). As effector cells, γδT intraepithelial lymphocytes express more Myo1e and Myo1f than their thymus and spleen counterpart.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Myo1f has emerged as an α-tubulin interacting protein relevant in the dynein-mediated Syk and CARD9 protein transport from the membrane to the cytoplasm during antifungal activation in macrophages ( 32 ). Finally, Myo1f regulates filopodia dynamics, increasing adhesion and migration ( 33 ). Together, previous reports suggest a role of Myo1f in the plasma membrane dynamics, cell adhesion, migration, and vesicular trafficking.…”

Section: Introductionmentioning

confidence: 99%

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Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

et al. 2023

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γδT intraepithelial lymphocyte represents up to 60% of the small intestine intraepithelial compartment. They are highly migrating cells and constantly interact with the epithelial cell layer and lamina propria cells. This migratory phenotype is related to the homeostasis of the small intestine, the control of bacterial and parasitic infections, and the epithelial shedding induced by LPS. Here, we demonstrate that Myo1f participates in the adhesion and migration of intraepithelial lymphocytes. Using long-tailed class I myosins KO mice, we identified the requirement of Myo1f for their migration to the small intestine intraepithelial compartment. The absence of Myo1f affects intraepithelial lymphocytes’ homing due to reduced CCR9 and α4β7 surface expression. In vitro, we confirm that adhesion to integrin ligands and CCL25-dependent and independent migration of intraepithelial lymphocytes are Myo1f-dependent. Mechanistically, Myo1f deficiency prevents correct chemokine receptor and integrin polarization, leading to reduced tyrosine phosphorylation which could impact in signal transduction. Overall, we demonstrate that Myo1f has an essential role in the adhesion and migration in γδT intraepithelial lymphocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

et al. 2023

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“…In this regard, inverse Myo1f and Tasp1 relative expression correlate with differentiation in stem cell-monocyte-macrophage lineage. Mechanistically, Myo1f is cleaved by the caspase 1 protease, which impaired its function, indicating a Tasp1 negative regulation of Myo1f during differentiation (Hensel A. et al, 2022). As effector cells, T intraepithelial lymphocytes express more Myo1e and Myo1f than their thymus and spleen counterpart.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Myo1f has emerged as an α-tubulin interacting protein relevant in the dynein-mediated Syk and CARD9 protein transport from the membrane to the cytoplasm during antifungal activation in macrophages (Sun et al, 2021). Finally, Myo1f regulates filopodia dynamics, increasing adhesion and migration (Hensel A. et al, 2022). Together, indicating the relevance of the role of Myo1f during the dynamics of the plasma membrane, cell adhesion and migration, and vesicular trafficking.…”

Section: Introductionmentioning

confidence: 99%

Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

Martínez-Vargas

Sánchez-Bello

Miguel-Rodríguez

et al. 2022

Preprint

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γδT intraepithelial lymphocyte represents up to 60% of the small intestine intraepithelial compartment. They are highly migrating cells and constantly interact with the epithelial cell layer and lamina propria cells. This migratory phenotype is related to the homeostasis of the small intestine, the control of bacterial and parasitic infections, and the epithelial shedding induced by LPS. Here, we demonstrate that Myo1f participates in the adhesion and migration of intraepithelial lymphocytes. Using long-tailed class I myosins KO mice, we identified the requirement of Myo1f for their migration to the small intestine intraepithelial compartment. The absence of Myo1f affects intraepithelial lymphocytes' homing due to reduced CCR9 and α4β7 expression. In vitro, we confirm that adhesion to integrin ligands and CCL25-dependent and independent migration of intraepithelial lymphocytes are Myo1f-dependent. Mechanistically, Myo1f deficiency prevents correct chemokine receptor and integrin polarization, leading to reduced tyrosine phosphorylation and having consequences in cell signaling. Overall, we demonstrate that Myo1f has an essential role in the adhesion and migration in γδT intraepithelial lymphocytes.

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Filopodia In Vitro and In Vivo

Blake

Gallop

2023

Annu. Rev. Cell Dev. Biol.

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Filopodia are dynamic cell surface protrusions used for cell motility, pathogen infection, and tissue development. The molecular mechanisms determining how and where filopodia grow and retract need to integrate mechanical forces and membrane curvature with extracellular signaling and the broader state of the cytoskeleton. The involved actin regulatory machinery nucleates, elongates, and bundles actin filaments separately from the underlying actin cortex. The refined membrane and actin geometry of filopodia, importance of tissue context, high spatiotemporal resolution required, and high degree of redundancy all limit current models. New technologies are improving opportunities for functional insight, with reconstitution of filopodia in vitro from purified components, endogenous genetic modification, inducible perturbation systems, and the study of filopodia in multicellular environments. In this review, we explore recent advances in conceptual models of how filopodia form, the molecules involved in this process, and our latest understanding of filopodia in vitro and in vivo. Expected final online publication date for the Annual Review of Cell and Developmental Biology, Volume 39 is October 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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The Taspase1/Myosin1f-axis regulates filopodia dynamics

Cited by 5 publications

References 50 publications

Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

Filopodia In Vitro and In Vivo

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