The taxonomic structure of southern chernozem at the genus level influenced by microbial preparations and farming systems

Abstract

The taxonomic structure of the microbiome of southern chernozem compared to virgin soil was studied in the stationary experiment on the use of microbial preparations in the conditions of traditional for the steppe part of the Crimea and each culture of the five-field crop rotation of the farming system and no-till. Among 823 genera it was revealed 7 dominant ones: Candidatus Nitrososphaera, Rubrobacter, Flavisolibacter, Segetibacter, Bacillus, Balneimonas, Skermanella. The low level of cellulose-decomposing bacteria of the genus Cellulomonas was remarked in southern chernozem. The highest proportion of the representatives of this genus was observed in virgin soil (0.55 %), while the farming systems contributed to its decline in 1.3-1.6 times. Microorganisms of the genera Erwinia and Xanthomonas with phytopathogenic properties were found with a minimum proportion in communities from 0.003 % to 0.014 % and 0 – 0.005%, respectively. The complex metagenomic analysis of the taxonomic structure of the soil microbiome allowed us to establish that the use of microbial preparations reduces the negative impact of the no-till farming system on the microbiocenosis of southern chernozem and improves its environmental feasibility in the Crimean steppe.

16S Amplicon Sequencing of Nitrifying Bacteria and Archaea Inhabiting Maize Rhizosphere and the Influencing Environmental Factors

“…The order Nitrosomonadaceae and Nitrosomonadales still have unclassified and yet to be cultured nitrifying bacteria species. The only archaea genus discovered was Candidatus Nitrososphaera, which carry out ammonia oxidation [39], which has been reported by Melnichuk, et al [40] and Enebe and Babalola [41] to be associated with crop plants including maize. The relative abundance of nitrite oxidizing genera were more than ammonia oxidizing genera (Figure 4).…”

State support of agricultural producers as a factor in increasing the competitiveness of the agricultural sector

“…Diagnostics of the tax policy of an enterprise by certain parameters allows to proceed to the next stage of assessing the tax competitiveness of an enterpriseidentifying the possibilities of the tax environment. At this stage, one should not merely diagnose such opportunities, but form an ideal tax policy for a business entity in certain conditions of the external tax environment (Melnichuk et al, 2020;Kazambayeva et al, 2019).…”