The taxonomy of the genus<i>Saccharomyces</i>meyen<i>ex</i>reess: A short review for non‐taxonomists

Barnett, James A.

doi:10.1002/yea.320080102

Cited by 134 publications

(70 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is due to the fact that the genus Saccharomyces has been frequently revised with respect to the composition of its members, and numerous species have been reclassified as synonyms (2). The realization that a single gene can confer on a yeast the ability to ferment or assimilate a sugar (19,50) raises the possibility that species might be inadvertently and artificially established simply on the basis of the function of one or a few genes (32).…”

mentioning

confidence: 99%

Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis

Lewicka¹,

Mallié²,

Bastide³

1995

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

Fifty-two strains of the genus Succhuromyces, including 50 yeasts of the Succharomyces sensu stricto complex (sensu Vaughan Martini 1989 [A. Vaughan Martini, Syst. Appl. Microbiol. 12119-122, 1989]), were analyzed for nine isozyme loci with starch gel electrophoresis. The genetic analysis of isozyme data provided further evidence that the Succhuromyces sensu stricto complex is a genetically heterogeneous group. Twenty-two different electrophoretic types were identified and could be grouped into four clusters. However, no correlation between these clusters and the currently defined species Succhuromyces cerevisiue, Succhuromyces buyunus, Succhuromyces pustoriunus, and Succharomyces paradom was detected. Several strains could not be assigned to their putative cluster.The criteria used in traditional yeast taxonomy are not always satisfactory for differentiation of species, in part because many separations are based on the presence or absence of assimilative abilities, which are often controlled by single mutable genes (15). Vaughan Martini (45) differentiated several species within the Saccharomyces sensu stricto group, while Yarrow (52) considered these as a single species (S. cerevisiae). This is a good example of this problem of strain variability in standard carbon assimilation and fermentation tests (3, 52). Other traditional methods also lead to ambiguous results (5).Recently there have been several reports on the use of molecular techniques to distinguish between strains of the Saccharomyces sensu stricto group. These criteria include levels of rRNA and DNA relatedness (16, 36,46), electrophoretic whole-cell protein patterns (44), restriction endonuclease patterns (17, 22, 28, 30), and electrophoretic karyotyping (23, 31,49). Using DNA-DNA reassociation, Vaughan Martini et al. (46,48) found that at least four species exist in the Saccharomyces sensu stricto complex: S. cerevisiae, S. bayanus, S. paradoxus, and S. pastotianus. This technique has been widely used to define species limits and relationships. However, it lacks the precision required for the analysis of genetic relationships among closely related strains (37). Analysis of whole-cell protein patterns and restriction enzyme analysis of genomic DNA or genes coding for rRNA were largely limited to differentiation of strains (12). Restriction enzyme analysis of mitochondrial DNA has given good results in the past (6, 22, 34) but requires a large number of screenings in order to define a suitable restriction enzyme. Recently, Guillamon et al. (9) have differentiated among the four species with four restriction enzymes. However, their results were not statistically checked.

show abstract

mentioning

confidence: 99%

Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis

Lewicka¹,

Mallié²,

Bastide³

1995

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…Our objective was to develop a quick pattern, but it was only specific for this strain since all other isolates of S. cerevisiae group gave a pattern, which was method for differentiating Saccharomyces sensu stricto and Torulaspora isolates, according to the accepted classification identical to that of the S. paradoxus type strain. This is a rare example of intraspecific variability of riboprinting patterns, system of these micro-organisms (Barnett et al 1990;Kurtzman and Robnett 1991;Barnett 1992), on the basis of their even in 25S rDNA, which was shown to be more conserved than 18S rDNA with the ITS region (Messner and Prillinger PCR riboprinting patterns. Using different restriction digestions of amplified 18S rDNA ¦ ITS1 and 25S rDNA frag-1995).…”

Section: Resultsmentioning

confidence: 98%

“…of these strains (Barnett 1992) as species of the Saccharomyces sensu stricto group as mentioned above. Both parts of the rDNA repeat were successfully amplified and found to be invariant in length for all tested yeasts.…”

Section: Resultsmentioning

confidence: 99%

Identification of Saccharomyces sensu stricto and Torulaspora yeasts by PCR ribotyping

Možina

Dlauchy

Deák

et al. 1997

Letters in Applied Microbiology

View full text Add to dashboard Cite

AK A ND P. R AS PO R . 1997. 18S rDNA ¦ ITS1 and 25S rDNA PCR products covering more than 95% of the nuclear ribosomal DNA repeat unit of 28 Saccharomyces sensu stricto and Torulaspora yeasts and their anamorph forms were digested with HaeIII, MspI, Hinf I and CfoI. Using combinations of two restriction enzymes, specific ribotyping patterns of six species were found. PCR ribotyping offers a convenient tool for quick identification of yeast isolates, but specificity of ribotyping patterns should be checked with a larger number of strains to avoid misidentification because of lack of variation within different taxa or because of strain-specific ribotyping patterns of species type strains.

show abstract

“…S. sensu stricto group include S. bayanus, S. cariocanus, S. cerevisiae, S. mikatae, S. paradoxus, S. pastorianus, S. kurdiavzevii and recently S. uvarum has been restored as a separate species 20 . S. sensu stricto strains can be distinguished by some physiological properties connected with beer production parameters: flocculation and sedimentation, resistance to ethanol or to high concentrations of sugars in wort 3,10,14 . Unfortunately, differentiation by classical methods is time consuming and sometimes leads to the contradictory results 29 .…”

Section: Introductionmentioning

confidence: 99%

PCR-Based Differentiation and Homology of Brewing and Type Strains of the Genus Saccharomyces

Barszczewski

Robak

2006

Journal of the Institute of Brewing

View full text Add to dashboard Cite

Restriction fragment length polymorphism (RFLP) patterns of PCR-amplified ribosomal RNA gene fragments (rDNA) and randomly amplified polymorphic DNA (RAPD) were applied for the analysis of 15 brewing and 6 related yeast strains of the genus Saccharomyces. One five-base (ScrFI) and two four-base cutting (HaeIII, MspI) restriction enzymes were used. The primers 21 and M13 core sequence were selected for RAPD analysis. PCR-RFLP rDNA analysis with HaeIII, ScrFI and MspI differentiated the strains tested into four, five and four types of patterns, respectively and the analyses of the profiles showed 100% homology, between the yeast strains. One strain was an exception. Homological groups were observed for strains used in breweries globally, from a local production strain and from the isolates identified as S. cerevisiae. Using RAPD analysis, and according to discrete differences in the profiles, it was possible to divide twenty one strains into 15 and 20 groups with primer 21 and M13 respectively. RFLP-PCR rDNA analysis was used to show similarities in closely related brewing strains, while RAPD analysis was used for differentiation of strains.

show abstract

The taxonomy of the genusSaccharomycesmeyenexreess: A short review for non‐taxonomists

Cited by 134 publications

References 100 publications

Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis

Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis

Identification of Saccharomyces sensu stricto and Torulaspora yeasts by PCR ribotyping

PCR-Based Differentiation and Homology of Brewing and Type Strains of the Genus Saccharomyces

Contact Info

Product

Resources

About