The Tc1/mariner DNA transposons in the genome of mollusk Littorina saxatilis

Puzakova, L. V.; Пузаков, М. В.

doi:10.1134/s1022795417120110

Cited by 2 publications

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“…Few DIRS1-like retrotransposons were detected in Aplysia californica and Lottia gigantea genomes [ 2 ]. Some recent studies of mollusc TEs refer to transposons (MITE Pearl in Crassostrea virginica [ 19 ], Tc1/mariner in Littorina saxatilis [ 20 ]) and novel superfamilies of SINE elements in gastropods and bivalves [ 21 , 22 ]. Considering LTR-retrotransposons, an active Gypsy element, Steamer, has been detected in leukemic cells of Mya arenaria [ 23 ] and thereafter observed in divers bivalves [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Mollusc genomes reveal variability in patterns of LTR-retrotransposons dynamics

et al. 2018

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BackgroundThe three superfamilies of Long Terminal Repeat (LTR) retrotransposons are a widespread kind of transposable element and a major factor in eukaryotic genome evolution. In metazoans, recent studies suggested that Copia LTR-retrotransposons display specific dynamic compared to the more abundant and diverse Gypsy elements. Indeed, Copia elements show a relative scarcity and the prevalence of only a few clades in specific hosts. Thus, BEL/Pao seems to be the second most abundant superfamily. However, the generality of these assumptions remains to be assessed. Therefore, we carried out the first large-scale comparative genomic analysis of LTR-retrotransposons in molluscs. The aim of this study was to analyse the diversity, copy numbers, genomic proportions and distribution of LTR-retrotransposons in a large host phylum.ResultsWe compare nine genomes of molluscs and further added LTR-retrotransposons sequences detected in databases for 47 additional species. We identified 1709 families, which enabled us to define 31 clades. We show that clade richness was highly dependent on the considered superfamily. We found only three Copia clades, including GalEa and Hydra which appear to be widely distributed and highly dominant as they account for 96% of the characterised Copia elements. Among the seven BEL/Pao clades identified, Sparrow and Surcouf are characterised for the first time. We find no BEL or Pao elements, but the rare clades Dan and Flow are present in molluscs. Finally, we characterised 21 Gypsy clades, only five of which had been previously described, the C-clade being the most abundant one. Even if they are found in the same number of host species, Copia elements are clearly less abundant than BEL/Pao elements in copy number or genomic proportions, while Gypsy elements are always the most abundant ones whatever the parameter considered.ConclusionsOur analysis confirms the contrasting dynamics of Copia and Gypsy elements in metazoans and indicates that BEL/Pao represents the second most abundant superfamily, probably reflecting an intermediate dynamic. Altogether, the data obtained in several taxa highly suggest that these patterns can be generalised for most metazoans. Finally, we highlight the importance of using database information in complement of genome analyses when analyzing transposable element diversity.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5200-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Mollusc genomes reveal variability in patterns of LTR-retrotransposons dynamics

et al. 2018

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“…Transposons were also recorded in the periwinkle genomes; in particular, Puzakova and Puzakov (2017) compared sequences in the L. saxatilis genome with known TEs and identified six sequences similar to the Tc1/mariner DNA transposons [ 54 ]. McInerney et al (2011) performed a comparative genomic analysis of microsatellite containing sequences (MCS) in L. saxatilis and L. littorea genomes.…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata

Lobov

Babkina

Данилов

et al. 2021

Biology

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Genus Littorina subgenus Neritrema (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (Littorina obtusata and L. fabalis). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of L. obtusata and L. fabalis by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between L. obtusata and L. fabalis, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the Littorina species divergence, which has not been reported previously.

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The Tc1/mariner DNA transposons in the genome of mollusk Littorina saxatilis

Cited by 2 publications

References 45 publications

Mollusc genomes reveal variability in patterns of LTR-retrotransposons dynamics

Mollusc genomes reveal variability in patterns of LTR-retrotransposons dynamics

Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata

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