The tea leaf microbiome shows specific responses to chemical pesticides and biocontrol applications

Cernava, Tomislav; Chen, Xiaoyulong; Krug, Lisa; Li, Haoxi; Yang, Maofa; Berg, Gabriele

doi:10.1016/j.scitotenv.2019.02.319

Cited by 53 publications

(33 citation statements)

References 58 publications

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“…is an economically important perennial crop plant mostly cultivated in the North-Eastern part in India. Extensive use of agrochemicals to meet the global requirement of tea resulted in an alteration of the microbial community associated with the tea plants 10 , 11 . Interestingly, little is known regarding the tea rhizosphere microbiome of Indian tea.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India

Bhattacharyya

Banerjee

Acharya

et al. 2020

Sci Rep

135

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A total of 120 rhizobacteria were isolated from seven different tea estates of Darjeeling, West Bengal, India. Based on a functional screening of in vitro plant growth-promoting (PGP) activities, thirty potential rhizobacterial isolates were selected for in-planta evaluation of PGP activities in rice and maize crops. All the thirty rhizobacterial isolates were identified using partial 16S rRNA gene sequencing. Out of thirty rhizobacteria, sixteen (53.3%) isolates belong to genus Bacillus, five (16.6%) represent genus Staphylococcus, three (10%) represent genus Ochrobactrum, and one (3.3%) isolate each belongs to genera Pseudomonas, Lysinibacillus, Micrococcus, Leifsonia, Exiguobacterium, and Arthrobacter. Treatment of rice and maize seedlings with these thirty rhizobacterial isolates resulted in growth promotion. Besides, rhizobacterial treatment in rice triggered enzymatic [ascorbate peroxidase (APX), catalase (CAT), chitinase, and phenylalanine ammonia-lyase (PAL)], and non-enzymatic [proline and polyphenolics] antioxidative defense reactions indicating their possible role in the reduction of reactive oxygen species (ROS) burden and thereby priming of plants towards stress mitigation. To understand such a possibility, we tested the effect of rhizobacterial consortia on biotic stress tolerance of rice against necrotrophic fungi, Rhizoctonia solani AG1-IA. Our results indicated that the pretreatment with rhizobacterial consortia increased resistance of the rice plants towards the common foliar pathogen like R. solani AG1-IA. This study supports the idea of the application of plant growth-promoting rhizobacterial consortia in sustainable crop practice through the management of biotic stress under field conditions.

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Section: Introductionmentioning

confidence: 99%

Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India

Bhattacharyya

Banerjee

Acharya

et al. 2020

Sci Rep

135

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“…Only a smaller fraction of the identified microorganisms in the product samples originates from the initial substrate (C. paliurus leaves). The leaves were shown to harbor a bacterial community that is similar to the phyllosphere microbiomes of the various plant species and especially to those of the perennial plants (e.g., Durand et al, 2018;Cernava et al, 2019a). Although feed is an important factor that shapes the gut microbiome of insects (Lewis and Lizé, 2015), it can be anticipated that only a smaller fraction can adapt to the varying conditions during the formation process of the insect tea.…”

Section: Discussionmentioning

confidence: 99%

Microbiome-Guided Exploration of the Microbial Assemblage of the Exotic Beverage “Insect Tea” Native to Southwestern China

Mao

Kusstatscher

Li³

et al. 2020

Front. Microbiol.

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“…In our experiment we investigated the effect of seed application of S. rhizophila SPA-P69 in combination with a fungicide, based on the active ingredients fludioxonil, metalaxyl-M, captan and ziram. Fungicide treatments were previously shown, apart from their effect on plant health, to also influence the rhizosphere and phyllosphere of plants [27]. We expect that also the SPA-P69 PGPR treatment induced a microbiome shift.…”

mentioning

confidence: 86%

Microbiome Management by Biological and Chemical Treatments in Maize is Linked to Plant Health

Kusstatscher¹,

Wicaksono²,

Thenappan³

et al. 2020

Preprint

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The targeted application of plant growth promoting rhizobacteria (PGPR) provides the key for a future sustainable agriculture with reduced pesticide application. PGPR interaction with the indigenous microbiota is poorly understood but essential to develop reliable applications. Therefore, Stenotrophomonas rhizophila SPA-P69 was applied as seed coating and in combination with a fungicide based on the active ingredients fludioxonil, metalaxyl-M, captan and ziram. Plant performance and rhizosphere composition of treated and non-treated maize plants of two field trials were analyzed. Plant health was significantly increased by treatment; however overall corn yield was not changed. By applying high-throughput amplicon sequencing of the 16S rRNA and the ITS genes, the bacterial and fungal changes in the rhizosphere due to different treatments were determined. Despite treatments had a significant impact on the rhizosphere microbiota (9- 12%), the field site was identified as main driver (27- 37%). Soil microbiota composition from each site was significantly different, which explains the site-specific effects. In this study we were able to show first indications how PGPR treatments increase plant health via microbiome shifts in a site-specific manner. This way first steps towards a detailed understanding of PGPRs and developments of consistently efficient applications in diverse environments are set.

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The tea leaf microbiome shows specific responses to chemical pesticides and biocontrol applications

Cited by 53 publications

References 58 publications

Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India

Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India

Microbiome-Guided Exploration of the Microbial Assemblage of the Exotic Beverage “Insect Tea” Native to Southwestern China

Microbiome Management by Biological and Chemical Treatments in Maize is Linked to Plant Health

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