The TEAD family transcription factor Scalloped regulates blood progenitor maintenance and proliferation in Drosophila through PDGF/VEGFR receptor (Pvr) signaling

Ferguson, Gabriel B.; Martínez‐Agosto, Julian A.

doi:10.1016/j.ydbio.2017.03.016

Cited by 27 publications

(30 citation statements)

References 33 publications

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“…Detailed temporal analysis of the dynamics of progenitor subpopulation during normal development, as well as upon loss of whd 1 ( Figure 2J–S ), was next carried out. In sync with an earlier report ( Ferguson and Martinez-Agosto, 2017 ), our analysis reveals that Dome - pre-progenitors ( Figure 2K ) are present in the developing lymph gland until the early third instar ( Figure 2L and Figure 2—figure supplement 1B ). Beyond this timeline, the subsets that populates the lymph gland are Dome + progenitors ( Figure 2M–N and Figure 2—figure supplement 1B ), and Dome + Pxn + IP cells ( Figure 2M–N and Figure 2—figure supplement 1B ).…”

Section: Resultssupporting

confidence: 91%

Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila

Tiwari

Toshniwal

Mandal

et al. 2020

eLife

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Cell-intrinsic and extrinsic signals regulate the state and fate of stem and progenitor cells. Recent advances in metabolomics illustrate that various metabolic pathways are also important in regulating stem cell fate. However, our understanding of the metabolic control of the state and fate of progenitor cells is in its infancy. Using Drosophila hematopoietic organ: lymph gland, we demonstrate that Fatty Acid Oxidation (FAO) is essential for the differentiation of blood cell progenitors. In the absence of FAO, the progenitors are unable to differentiate and exhibit altered histone acetylation. Interestingly, acetate supplementation rescues both histone acetylation and the differentiation defects. We further show that the CPT1/whd (withered), the rate-limiting enzyme of FAO, is transcriptionally regulated by Jun-Kinase (JNK), which has been previously implicated in progenitor differentiation. Our study thus reveals how the cellular signaling machinery integrates with the metabolic cue to facilitate the differentiation program.

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Section: Resultssupporting

confidence: 91%

Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila

Tiwari

Toshniwal

Mandal

et al. 2020

eLife

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“…Discovery of the hidden cell population-PH1, will shed light on understanding the hierarchy of prohemocyte differentiation and enhance the relevance of the lymph gland as a hematopoietic model. Roles for Notch, Stat92E, or scalloped in the earliest state of prohemocytes have been previously suggested by recent studies 18,[48][49][50] . Moreover, clonal analyses have shown that cells adjacent to the PSC generate the largest population in the lymph gland 51 .…”

Section: Discussionmentioning

confidence: 69%

Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila

et al. 2020

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The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.

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“…In Drosophila , sd mutants exhibit abnormal wings with wing margin gaps and ectopic bristles (Kiso, ; Campbell et al ., ; Campbell et al ., ). sd overexpression promotes tissue overgrowth, while sd inactivation decreases organ size, including neuroblasts, brain, eyes, and wing discs in Drosophila melanogaster (Poon et al ., ; Boone et al ., ; Ferguson & Martinez‐Agosto, ). Additionally, sd affects the development of various Drosophila organs, such as the eyes, legs, optic lobe (Garg et al ., ), sensory bristles (Anand et al ., ), trachea (Robbins et al ., ), and lymph glands (Ferguson & Martinez‐Agosto, , ).…”

Section: Introductionmentioning

confidence: 99%

BmSd gene regulates the silkworm wing size by affecting the Hippo pathway

Yin

Zhang

et al. 2019

Insect Science

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Insect wings are developed from the wing disc during metamorphosis. Bombyx mori, a model lepidopteran insect, loses flight ability after long-term domestication from the wild silkworm, Bombyx mandarina. The mw mutant (u11 strain) shows minute wings compared to wild type (e.g., p50 strain) wings. RNA sequencing analysis previously revealed differential Hippo-pathway-related gene expression between the u11 and p50 strains. The Hippo pathway is an evolutionarily conserved signaling cascade that controls organ size during development in animals. In this study, the function of BmSd which has been characterized as one of the Hippo-pathway-related genes was analyzed for silkworm wing development. We found that mats, warts, and hippo expression levels were higher in u11 compared to p50 wing discs. BmSd (scalloped) expression, which encodes a prominent transcriptional partner to Yorkie (Yki), gradually decreased during the wandering stage in u11, but exhibited the opposite expression pattern in p50. When BmSd was knocked down by small interfering RNA during the wandering stage in the p50 strain, 57.9% of the individuals showed minute wings. Additionally, ex, kibra, and wingless expression levels decreased in the BmSd knockdown mutant. Further, BmSd deletion mediated by clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated protein 9 induced 50% of individuals with minute wings, a phenotype similar to the mw mutant. This result demonstrates that BmSd plays pivotal roles in silkworm wing development. Our results show that the Hippo signaling pathway participates and plays crucial roles in the regulation of silkworm wing development, and our findings provide a basis for further research on B. mori wing development.

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The TEAD family transcription factor Scalloped regulates blood progenitor maintenance and proliferation in Drosophila through PDGF/VEGFR receptor (Pvr) signaling

Cited by 27 publications

References 33 publications

Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila

Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila

Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila

BmSd gene regulates the silkworm wing size by affecting the Hippo pathway

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