The TEAD4–YAP/TAZ Protein–Protein Interaction: Expected Similarities and Unexpected Differences

Hau, Jean Christophe; Erdmann, Dirk; Mesrouze, Yannick; Furet, Pascal; Fontana, Patrizia; Zimmermann, Catherine; Schmelzle, Tobias; Hofmann, Francesco; Chêne, Patrick

doi:10.1002/cbic.201300163

Cited by 68 publications

(130 citation statements)

References 34 publications

Supporting

Mentioning

122

Contrasting

Order By: Relevance

“…Although YAP and TAZ have essentially the same affinity for TEAD (TEA domain family member 1), differences have been found in the ways in which they interact with TEAD (56). Recent evidence suggests that YAP and TAZ do not compensate for each other (57), as evidenced by the findings that YAP and TAZ knockout mice show different phenotypes and that the phenotypes of YAP and TAZ knockdowns cannot be compensated for by the other gene (58).…”

Section: Discussionmentioning

confidence: 99%

Adhesion Molecule-Mediated Hippo Pathway Modulates Hemangioendothelioma Cell Behavior

Madri

2014

Molecular and Cellular Biology

View full text Add to dashboard Cite

Hemangioendotheliomas are categorized as intermediate-grade vascular tumors that are commonly localized in the lungs and livers. The regulation of this tumor cell's proliferative and apoptotic mechanisms is ill defined. We recently documented an important role for Hippo pathway signaling via endothelial cell adhesion molecules in brain microvascular endothelial cell proliferation and apoptosis. We found that endothelial cells lacking cell adhesion molecules escaped from contact inhibition and exhibited abnormal proliferation and apoptosis. Here we report on the roles of adherens junction molecule modulation of survivin and the Hippo pathway in the proliferation and apoptosis of a murine hemangioendothelioma (EOMA) cell. We demonstrated reduced adherens junction molecule (CD31 and VE-cadherin) expression, increased survivin and Ajuba expression, and a reduction in Hippo pathway signaling resulting in increased proliferation and decreased activation of effector caspase 3 in postconfluent EOMA cell cultures. Furthermore, we confirmed that YM155, an antisurvivin drug that interferes with Sp1-survivin promoter interactions, and survivin small interference RNA (siRNA) transfection elicited induction of VE-cadherin, decreased Ajuba expression, increased Hippo pathway and caspase activation and apoptosis, and decreased cell proliferation. These findings support the importance of the Hippo pathway in hemangioendothelioma cell proliferation and survival and YM155 as a potential therapeutic agent in this category of vascular tumors.

show abstract

Section: Discussionmentioning

confidence: 99%

Adhesion Molecule-Mediated Hippo Pathway Modulates Hemangioendothelioma Cell Behavior

Madri

2014

Molecular and Cellular Biology

View full text Add to dashboard Cite

show abstract

“…Although much of the TEAD-binding domain is conserved between TAZ and YAP, TAZ lacks the PxxΦP motif. Thus, differences between TAZ and YAP binding to TEAD transcription factors exist, which is supported by recent molecular modeling studies and mutational analysis (Hau et al, 2013). However, whether these differences translate to disparate TAZ/YAP activities in vivo is yet to be determined.…”

Section: Box 1 Evolutionary Conservation Of the Hippo Pathwaymentioning

confidence: 93%

The Hippo pathway effectors TAZ and YAP in development, homeostasis and disease

2014

View full text Add to dashboard Cite

Studies over the past 20 years have defined the Hippo signaling pathway as a major regulator of tissue growth and organ size. Diverse roles for the Hippo pathway have emerged, the majority of which in vertebrates are determined by the transcriptional regulators TAZ and YAP (TAZ/YAP). Key processes regulated by TAZ/YAP include the control of cell proliferation, apoptosis, movement and fate. Accurate control of the levels and localization of these factors is thus essential for early developmental events, as well as for tissue homeostasis, repair and regeneration. Recent studies have revealed that TAZ/YAP activity is regulated by mechanical and cytoskeletal cues as well as by various extracellular factors. Here, I provide an overview of these and other regulatory mechanisms and outline important developmental processes controlled by TAZ and YAP.

show abstract

“…YAP1, a transcription factor, is an ovarian cancer oncogene in the previous studies, but the core of the mammalian Hippo pathway is composed of two kinases complexes: the MST1-2/Sav1 complex and the LATS2/MOB complex [21,39,40]. The previous study showed that YAP1 has no transcriptional activity and its actions are dependent on downstream transcriptional factors [41,42]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Hippo Signaling Pathway Reveals a Spatio-Temporal Correlation with the Size of Primordial Follicle Pool in Mice

Xiang

et al. 2015

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: The Hippo signaling pathway, a highly conserved cell signaling system, exists in most multicellular organisms and regulates cell proliferation, differentiation, and apoptosis. It has been reported that the members of Hippo signaling are expressed in mammalian ovaries, but the exact functions of this pathway in primordial follicle development remains unclear. Methods: To analyze the spatio-temporal correlation between the core component of Hippo pathway and the size of primordial follicle pool, Western blot, Real-time PCR and immunohistochemistry were used, and the expression and localization of MST1, LATS2 and YAP1 mRNA and protein were examined in 3 d, 1 m, 5 m, 16 m postnatal mice ovary and the culture model of mice primordial follicle in vitro. Results: Both the protein and mRNA expression of the MST1 and LATS2 were decreased significantly as mouse age increased (p < 0.05), however, the mRNA expression of them increased significantly in 16 m compared with 5 m as well as the protein expression of LATS2.The expression of YAP showed the opposite trend, and the significant protein expression of pYAP was increased before 1 m, after which no significant change was observed. Moreover, the ratio of pYAP/YAP decreased significantly. Culturing ovaries for 8 d in vitro resulted in the activation of primordial follicles in 3 d postnatal mice ovaries, and these developed into primary follicles with the expression of PCNA increasing significantly (p < 0.05). The mRNA and protein expression of MST and LATS decreased significantly (p < 0.05), and the expression of YAP increased significantly (p < 0.05, p < 0.01), whereas the ratio of pYAP/YAP decreased significantly (p < 0.05). Conclusion: The above results reveal that the expression of the core components of Hippo pathway changed during mouse follicular development, especially before and after primordial follicle activation in vitro. The primordial follicle activation may be related to the significant decrease of the ratio of pYAP1/YAP1. In conclusion, Hippo signaling pathway expressed in mice ovaries and have spatio-temporal correlation with the size of primordial follicle pool.

show abstract

The TEAD4–YAP/TAZ Protein–Protein Interaction: Expected Similarities and Unexpected Differences

Cited by 68 publications

References 34 publications

Adhesion Molecule-Mediated Hippo Pathway Modulates Hemangioendothelioma Cell Behavior

Adhesion Molecule-Mediated Hippo Pathway Modulates Hemangioendothelioma Cell Behavior

The Hippo pathway effectors TAZ and YAP in development, homeostasis and disease

Hippo Signaling Pathway Reveals a Spatio-Temporal Correlation with the Size of Primordial Follicle Pool in Mice

Contact Info

Product

Resources

About