The teleost sperm membrane progestogen receptor: Interactions with xenoestrogens

Thomas, Peter; Breckenridge-Miller, D.; Detweiler, Charles

doi:10.1016/s0141-1136(97)00090-1

Cited by 27 publications

(23 citation statements)

References 5 publications

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“…Finally, there is a growing body of evidence that xenobiotic chemicals which cause endocrine disruption through binding to nuclear receptors can also interfere with steroid actions mediated through membrane receptors including mPRs and GPR30 [36,184,214,217,218,225,233,257]. Therefore, it is important that environmental toxicologists and regulatory agencies also consider endocrine disruption through interference with nonclassical steroid actions when investigating environmental contaminants and making regulatory decisions.…”

Section: Discussionmentioning

confidence: 99%

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Thomas

2012

General and Comparative Endocrinology

147

105

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In addition to the classic genomic mechanism of steroid action mediated by activation of intracellular nuclear receptors, there is now extensive evidence that steroids also activate receptors on the cell surface to initiate rapid intracellular signaling and biological responses that are often nongenomic. Recent progress in our understanding of rapid, cell surface-initiated actions of estrogens, progestins, androgens and corticosteroids and the identities of the membrane receptors that act as their intermediaries is briefly reviewed with a special emphasis on studies in teleost fish. Two recently discovered novel proteins with seven-transmembrane domains, G protein-coupled receptor 30 (GPR30), and membrane progestin receptors (mPRs) have the ligand binding and signaling characteristics of estrogen and progestin membrane receptors, respectively, but their functional significance is disputed by some researchers. GPR30 is expressed on the cell surface of fish oocytes and mediates estrogen inhibition of oocyte maturation. mPRα is also expressed on the oocyte cell surface and is the intermediary in progestin induction of oocyte maturation in fish. Recent results suggest there is cross-talk between these two hormonal pathways and that there is reciprocal down-regulation of GPR30 and mPRα expression by estrogens and progestins at different phases of oocyte development to regulate the onset of oocyte maturation. There is also evidence in fish that mPRs are involved in progestin induction of sperm hypermotility and anti-apoptotic actions in ovarian follicle cells. Nonclassical androgen and corticosteroid actions have also been described in fish models but the membrane receptors mediating these actions have not been identified.

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Section: Discussionmentioning

confidence: 99%

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Thomas

2012

General and Comparative Endocrinology

147

105

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“…For example, Kepone and o,p-DDD bind to the plasma membrane receptor for the progestational maturation-inducing steroid in ovaries of spotted sea trout (Das & Thomas 1999). Kepone can also inhibit the gonadotropin-stimulated androgen production in testicular tissue from Atlantic croaker by an estrogen membrane receptor (Thomas et al 1998). The nongenomic action of a progestin on sperm motility in Atlantic croaker can be partially or completely blocked by o,p 0 -DDT, Kepone, chlordane, methoxychlor and p,p 0 -DDT at concentrations of 0.01-10 mM (Thomas & Doughty 2004).…”

Section: Discussionmentioning

confidence: 99%

Rapid effects of pesticides on human granulosa-lutein cells

Y¹,

Foster²,

Younglai³

2006

Reproduction

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“…Certain metabolites of the pesticide DDT were found to bind to a membrane estrogen receptor in the testis of the Atlantic croaker [31], and o,pЈ-DDE was found to bind to the membrane progestogen receptor on croaker sperm [44]. In addition, o,pЈ-DDD was found to bind to the membrane MIS receptor in croaker ovaries and to disrupt the process of final oocyte maturation (for review, see [45]).…”

Section: Discussionmentioning

confidence: 99%

Biochemical Characterization of a Membrane Androgen Receptor in the Ovary of the Atlantic Croaker (Micropogonias undulatus)1

Braun¹,

Thomas²

2004

Biology of Reproduction

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Membrane androgen receptors have been biochemically characterized in only a few vertebrate species to date. Therefore, the purpose of the current study was to comprehensively investigate the binding characteristics of a putative membrane androgen receptor in the ovary of the teleost, Atlantic croaker (Micropogonias undulatus). Specific androgen binding to an ovarian plasma membrane fraction was demonstrated using a radioreceptor assay protocol consisting of a short-term incubation with [(3)H]testosterone (T) and subsequent filtration of bound steroid from free steroid. Saturation and Scatchard analyses of T binding to an ovarian plasma membrane fraction indicated the presence of a single, high-affinity (K(d) = 15.32 +/- 2.68 nM [mean +/- SEM]), low-capacity (B(max) = 2.81 +/- 0.31 pmol/mg protein), androgen-binding site. Specific androgen binding to the receptor was readily displaceable, and the association and dissociation kinetics were rapid (half-time = 3.7 +/- 1.7 and 4.7 +/- 0.2 min, respectively). Competitive binding assays showed that 5alpha-dihydrotestosterone, T, and 11-ketotestosterone had relative binding affinities (RBAs) of 193%, 100%, and 13%, respectively, whereas none of the C(18) or C(21) steroids tested bound with high affinity except for progesterone (RBA = 191%). This androgen-binding moiety with high affinity for progesterone is unlikely to mediate the physiological actions of progestins in croaker, because it has low binding affinity for fish progestin hormones. Androgen-binding sites were also detected in membrane fractions of the brain, liver, kidney, and drumming muscle, whereas little or no binding was detected in the trunk muscle, heart, gills, or intestine. Receptor levels increased 10-fold during ovarian recrudescence, reaching maximum levels in fully mature ovaries, which suggests a likely physiological role for this receptor during the reproductive cycle of female croaker. It is concluded that the androgen-binding moiety identified in the plasma membrane fraction of Atlantic croaker ovarian tissue fulfils all the criteria for its designation as a steroid receptor.

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The teleost sperm membrane progestogen receptor: Interactions with xenoestrogens

Cited by 27 publications

References 5 publications

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Rapid effects of pesticides on human granulosa-lutein cells

Biochemical Characterization of a Membrane Androgen Receptor in the Ovary of the Atlantic Croaker (Micropogonias undulatus)1

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