The temporal and spatial changes of actin cytoskeleton in the hippocampal CA1 neurons following transient global ischemia

Guo, Cheng; Xiong, Tianqing; Tan, Bai-Hong; Gui, Yue; Ye, Ning; Li, Shu-Lei; Li, Yanchao

doi:10.1016/j.brainres.2019.06.016

Cited by 18 publications

(14 citation statements)

References 42 publications

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“…Prior to the loss of CA1 neurons, extensive deletion of dendritic spines has been widely reported in the CA1 region (Johansen et al 1984;Yamamoto et al 1986; Kovalenko et al 2006). In line with this observation, lamentous actin (F-actin) within the dendritic spines was progressively depolymerized in this region after global cerebral ischemia (Guo et al 2019). As the only cytoskeletal component in the dendritic spines (Hotulainen and Hoogenraad 2010;Freire-Cobo et al 2014), the destruction of F-actin inevitably leads to a volume collapse of the dendritic spines.…”

Section: Introductionmentioning

confidence: 64%

“…Two-month-old Wistar rats (male, 250 ~ 270g) were used to induce transient global cerebral ischemia with a four-vessel occlusion method (Pulsinelli and Brierley, 1979;Guo et al, 2019.…”

Section: Induction Of Ischemia In Ratsmentioning

confidence: 99%

“…For uorescence analysis, day 1.5 after I/R was selected as a starting time point according to the results reported by Guo et al (2019). The rats were xed with 4% paraformaldehyde at day 1.5, 2, 3, 7 after I/R, and the brains were removed and cut into 30-μm-thick section, as described in our previous papers (Guo et al, 2019, Xiong et al, 2020. The protein levels of MAP2, PSD95 and synapsin I in the CA1 region were detected with SDS electrophoresis and western blots.…”

Section: Sample Preparationsmentioning

confidence: 99%

“…Alexa Fluor 488-conjugated donkey anti-mouse or donkey anti-rabbit IgG (Molecular Probes, Eugene, OR) was used to visualize mouse or rabbit primary antibodies. The staining procedure has been described in more detail in our previous papers (Xiong et al 2015Guo et al, 2019).…”

Section: Determination Of F-actin/g-actin (F/gmentioning

confidence: 99%

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Adaptive changes of presynaptic components in hippocampal CA1 subfield after transient global cerebral ischemia

Zhang

Tan

et al. 2021

Preprint

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Transient global cerebral ischemia induces acute loss of dendritic spines of CA1 pyramidal neurons in the hippocampus. On the other hand, it is unclear how the presynaptic terminals, which had lost their postsynaptic contacts, are persistently preserved after ischemia. We modeled global cerebral ischemia with two-stage 4-vessel-occlusion in rats, and found that three postsynaptic markers, MAP2, PSD95, and F-actin, were all severely decreased in area CA1 after ischemia/reperfusion (I/R). No significant change was detected for synapsin I, a presynaptic marker, at the protein level in the CA1 region after I/R. However, the puncta size of synapsin I became slightly, but significantly reduced in the early stage of I/R. As time went on, the puncta number of synapsin I became moderately decreased, while the puncta size of synaspin I was significantly increased. Interestingly, some enlarged puncta of synapsin I were observed to terminate directly onto the dendritic shafts of CA1 pyramidal cells. Due to a severe decrease of F-actin in the dendritic spines, the ratio of synapsin I/F-actin puncta number became significantly increased after I/R. The decrease in puncta size of synapsin I in the early stage of I/R may be the result of excessive release of synaptic vesicles due to I/R-induced hyperexcitability in CA3 pyramidal cells, while the increase in puncta size of synapsin I in the later stage of I/R may reflect the disability of synaptic vesicle release due to the loss of postsynaptic contacts.

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Section: Introductionmentioning

confidence: 64%

“…Two-month-old Wistar rats (male, 250 ~ 270g) were used to induce transient global cerebral ischemia with a four-vessel occlusion method (Pulsinelli and Brierley, 1979;Guo et al, 2019.…”

Section: Induction Of Ischemia In Ratsmentioning

confidence: 99%

Section: Sample Preparationsmentioning

confidence: 99%

Section: Determination Of F-actin/g-actin (F/gmentioning

confidence: 99%

See 2 more Smart Citations

Adaptive changes of presynaptic components in hippocampal CA1 subfield after transient global cerebral ischemia

Zhang

Tan

et al. 2021

Preprint

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“…In addition, endothelin release, leukocyte, and platelet activation, delayed coagulation, and endothelial dysfunction are involved in pathophysiological changes (33,34). The ischemia results in astrocyte function impairment, an increased glutamate release, and disruption of glutamate uptake in astrocytes (35)(36)(37)(38). Mechanisms such as the lipid peroxidation of calcium, the activation of proteolytic enzymes, free radical production, and gene activation result in an increase in irreversible neuronal damage (33,39).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Rifampicin on Experimental Cerebral Ischemia/Reperfusion Injury in Rats

Arslan

Gel

Demir

et al. 2019

Iran Red Crescent Med J

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Background: Ischemic brain damage can be explained by the emergence of acute focal or global neurological findings caused by vascular occlusions or hemorrhages. Even in non-fatal cases, stroke is an important pathologic condition with a severe impact on the quality of life, and patients require considerable assistance in the daily lives. Objectives: The purpose of this study was to investigate the effect of rifampicin on malondialdehyde (MDA) levels and neurological examination of the hippocampal region in rats with transient cerebral ischemia. Methods: This experimental study has been performed in a university-affiliated animal lab, Trabzon, Turkey, in 2016. Thirty-eight Sprague Dawley rats weighing 220-280 g were used. In this two-vessel occlusion and hypotension ischemia-reperfusion model, the bilateral carotid arteries were temporarily clipped (30 minutes), and blood was withdrawn up to 3 mL of intracardiac volume before the induction of hypotension. After 30 minutes, the clips were removed, and a reperfusion medium was created. One group of 12 rats received intraperitoneal injections of 30 mg/kg of rifampicin every day, and after a 30-minute bilateral carotid artery clipping and hypotension (10 mL/kg). Another group of 12 rats underwent a 30-minute bilateral carotid artery clipping and hypotension (10 mL/kg). The third group consisting of 7 rats underwent skin laceration only. The final group of 7 rats received anesthesia for only 15 minutes. Neurological examinations were performed at the end of days 1, 4, 7, and 10 in all groups. At the end of the 10th day, the animals were euthanized, and their brain tissues were removed. The hippocampi were removed from the brains for biochemical analysis and stored at-76°C in a deep freeze. Ischemic changes in the brain were assessed biochemically by measuring MDA levels in both blood and brain tissue. Results: There was no statistically significant difference between the groups in terms of the mean tissue MDA levels (P = 0.112), but a significant difference was determined in the mean serum MDA values (P = 0.033). Serum MDA values significantly differed between the Group 1 and Group 2 (P = 0.030), but not between Group 1 and Group 3 (P = 0.58). Serum MDA values were also significantly different between Group 2 and Group 3 (P = 0.019), and between Group 2 and Group 4 (P = 0.035). Conclusions: Rifampicin could exhibit a neuroprotective effect on cerebral ischemia-reperfusion injury.

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Short‐term effects of therapeutic hypothermia following hypoxia–ischemia in neonatal male and female rats

Zang

Colucci

Tassinari

et al. 2022

Intl J of Devlp Neuroscience

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Nowadays, the only treatment for human babies suffering from hypoxiaischemia (HI) is therapeutic hypothermia (TH). However, a better understanding of the specific effects of TH in males and females is important to improve its clinical application. The present study evaluated the short-term effects of TH on the brain injury and behavioral outcomes in male and female neonatal rats submitted to neonatal HI. Seven-day-old Wistar rats underwent a surgery for unilateral occlusion of the right common carotid artery and were exposed to a hypoxic atmosphere (8% oxygen) for 75 min. Then, the animals in the TH group were submitted to TH (scalp temperature of 32 C) for 5 h. In the behavioral tests, no remarkable differences triggered by HI or TH were observed relative to SHAM animals. Only females of the HI group presented lower latency to complete the righting reflex test. TH reduced the volume of brain injury in males, but not in females. The animals of the HI group showed a reduction in the number of neurons in the CA1 and dentate gyrus (DG) regions of the hippocampus and TH partially prevented neuronal death. In the CA1 region of the hippocampus, animals from the HI group showed more degenerating cells relative to the SHAM, which was reversed by TH. In the DG, animals from the HI group showed an increase in the number of degenerating neurons, which was partially reversed by TH only in males. Our data show that HI leads to a brain injury, which was attenuated by TH in a sex-dependent way and clarify the importance of the assessment of males and females in order to outline specific strategies for the treatment of each sex in newborns suffering from HI.

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The temporal and spatial changes of actin cytoskeleton in the hippocampal CA1 neurons following transient global ischemia

Cited by 18 publications

References 42 publications

Adaptive changes of presynaptic components in hippocampal CA1 subfield after transient global cerebral ischemia

Adaptive changes of presynaptic components in hippocampal CA1 subfield after transient global cerebral ischemia

The Effects of Rifampicin on Experimental Cerebral Ischemia/Reperfusion Injury in Rats

Short‐term effects of therapeutic hypothermia following hypoxia–ischemia in neonatal male and female rats

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