The temporal profiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat

Zhang, Ruilan; Chopp, Michael; Zaloga, Cecylia; Zhang, Zheng G.; Jiang, Ning; Gautam, Subhash C.; Tang, Wenxue; Tsang, Wayne; Anderson, Donald C.; Manning, Anthony M.

doi:10.1016/0006-8993(95)00346-r

Cited by 142 publications

(66 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Expression of chemotactic factors such as monocyte chemoattractant protein 1 is increased in the damaged CNS tissues (Kim, 1996), and injured tissue extract selectively induces chemotaxis of mesenchymal progenitors in vitro (Chen et al, 2001). Adhesion molecules such as intercellular adhesion molecule (Zhang et al, 1995), vascular adhesion molecule 1, and E-selectin are also highly expressed on the endothelial cells in the damaged lesions (Blann et al, 1990;Zhang et al, 1995;Haraldsen et al, 1996;Quesenberry and Becker, 1998). Other unknown molecules may also promote migration of transplanted bone marrow cells into the demyelinated lesions.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord

Inoue

Honmou

Oka

et al. 2003

Glia

102

View full text Add to dashboard Cite

The remyelinating potential of autologous bone marrow cells was studied after direct injection and following intravenous injection into rats with a demyelinated lesion in the spinal cord. Both focal and intravenous injections of acutely isolated mononuclear bone marrow cell fractions resulted in varying degrees of remyelination. Suspensions of bone marrow cells collected from the same rat were delivered at varied concentrations (10 2 to 10 5 for direct injection and 10 4 to 10 7 for i.v. injections). The lesions were examined histologically 3 weeks after transplantation. Light microscopic examination revealed remyelination in the dorsal funiculus with both injection protocols, but the extent of remyelination was proportional to the number of injected cells. To attain the same relative density of remyelination achieved by direct injection, intravenous administration of cells required delivery of substantially more cells (two orders of magnitude). However, the availability of autologous bone marrow cells in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggest the potential utility of intravenous cell delivery as a prospective therapeutic approach in demyelinating disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord

Inoue

Honmou

Oka

et al. 2003

Glia

102

View full text Add to dashboard Cite

show abstract

“…The expression of ICAM-1 was detected with immunohistochemistry and Western blotting 48 h after occlusion (15). Fig.…”

Section: Effect Of Prog On the Expression Of Icam-1 After Pmcao In Ratsmentioning

confidence: 99%

Progesterone inhibits inflammatory response pathways after permanent middle cerebral artery occlusion in rats

et al. 2011

View full text Add to dashboard Cite

Abstract. recent studies have indicated that progesterone (PROG) reduces the expression of inflammatory factors in brain tissue. The present study was designed to investigate whether PROG inhibits the inflammatory response and the infiltration of inflammatory cells in the brain. One hundred and seventy-six adult male Sprague-Dawley rats were randomly divided into four groups: a sham-operated (control) group, a permanent middle cerebral artery occlusion (pMCAO) group, a vehicle-treated group and a PROG-treated group. After pMCAO, the rats received an initial intraperitoneal injection of PROG (8 mg/kg) or vehicle at 1 h post-occlusion, followed by subcutaneous injections at 6, 24 and 48 h. The expression levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and CD68 in the brain were measured by immunohistochemistry and Western blotting. The level of myeloperoxidase (MPO) activity was detected by spectrophotometry. The rats were sacrificed 72 h after surgery and isolated brain was sectioned into coronal slices and stained with 2,3,5-triphenyltetrazolium chloride (TTC). Western blotting and spectrophotometry revealed that the expression levels of ICAM-1, VCAM-1, CD68 and MPO were reduced in the brain tissue of PROG-treated rats. In addition, PROG-treated rats showed a substantial reduction in the infarct volume compared to vehicle controls. PROG effectively inhibited the inflammatory response and reduced the infiltration of leukocytes in the ischemic brain by inhibiting the expression of ICAM-1 and VCAM-1.

show abstract

“…6 Intercellular adhesion molecule-1 levels have been elevated as early as 4 hours after stroke with sustained levels for up to 1 week. 95 Monocyte chemoattractant protein-1, a key chemoattractant factor for the recruitment of circulating peripheral cells to the stroke area and an important factor for stem cell migration, is upregulated 3 days after stroke and then returns to baseline after 1 week. 91 Similarly, SDF-1 is known to be a potent chemoattractant for inflammatory as well as stem cells (including BMSCs and NSCs) and is expressed early after stroke.…”

Section: Early Intravascular Cell Deliverymentioning

confidence: 99%

Intravascular cell replacement therapy for stroke

Guzman

Choi²,

Gera³

et al. 2008

FOC

View full text Add to dashboard Cite

✓ The use of stem cell transplantation to restore neurological function after stroke is being recognized as a potential novel therapy. Before stem cell transplantation can become widely applicable, however, questions remain about the optimal site of delivery and timing of transplantation. In particular, there seems to be increasing evidence that intravascular cell delivery after stroke is a viable alternative to intracerebral transplantation. In this review, the authors focus on the intravascular delivery of stem cells for stroke treatment with an emphasis on timing, transendothelial migration and possible mechanisms leading to neuroprotection, angiogenesis, immunomodulation, and neural plasticity. They also review current concepts of in vivo imaging and tracking of stem cells after stroke.

show abstract

The temporal profiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat

Cited by 142 publications

References 33 publications

Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord

Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord

Progesterone inhibits inflammatory response pathways after permanent middle cerebral artery occlusion in rats

Intravascular cell replacement therapy for stroke

Contact Info

Product

Resources

About