<p><strong>Abstract.</strong> Nitrous oxide (N<sub>2</sub>O) is the primary atmospheric constituent involved in stratospheric ozone depletion and contributes strongly to changes in the climate system through a positive radiative forcing mechanism. The atmospheric abundance of N<sub>2</sub>O has increased from 270&#8201;ppb during the pre-industrial era to approx. 330&#8201;ppb in 2018. Even though it is well known that microbial processes in agricultural and natural soils are the major N<sub>2</sub>O source, the contribution of specific soil processes is still uncertain. The relative abundance of N<sub>2</sub>O isotopocules (<sup>14</sup>N<sup>14</sup>N<sup>16</sup>N, <sup>14</sup>N<sup>15</sup>N<sup>16</sup>O, <sup>15</sup>N<sup>14</sup>N<sup>16</sup>O and <sup>14</sup>N<sup>14</sup>N<sup>18</sup>O) carries process-specific in-formation and thus can be used to trace production and consumption pathways. While isotope ratio mass spectroscopy (IRMS) was traditionally used for high-precision measurement of the isotopic composition of N<sub>2</sub>O, quantum cascade laser absorption spectroscopy (QCLAS) has been put forward as a complementary technique with the potential for on-site analysis. In recent years, preconcentration combined with QCLAS has been presented as a technique to resolve subtle changes in ambient N<sub>2</sub>O isotopic composition.</p> <p>From the end of May until the beginning of August 2016, we investigated N<sub>2</sub>O emissions from an intensively managed grassland at the study site Fendt in Southern Germany. In total, 612 measurements of ambient N<sub>2</sub>O were taken by combining preconcentration with QCLAS analyses, yielding &#948;<sup>15</sup>N<sup>&#945;</sup>, &#948;<sup>15</sup>N<sup>&#946;</sup>, &#948;<sup>18</sup>O and N<sub>2</sub>O concentration with a temporal resolution of approximately one hour and precisions of 0.46&#8201;&#8240;, 0.36&#8201;&#8240;, 0.59&#8201;&#8240; and 1.24&#8201;ppb, respectively. Soil &#948;<sup>15</sup>N-NO<sub>3</sub><sup>&#8722;</sup> values and concentrations of NO<sub>3</sub><sup>&#8722;</sup> and NH<sub>4</sub><sup>+</sup> were measured to further constrain possible N<sub>2</sub>O-emitting source processes. Furthermore, the concentration footprint area of measured N<sub>2</sub>O was determined with a Lagrangian particle dispersion model (FLEXPART-COSMO) using local wind and turbulence observations. These simulations indicated that night-time concentration observations were largely sensitive to local fluxes. While bacterial denitrification and nitrifier denitrification were identified as the primary N<sub>2</sub>O-emitting processes, N<sub>2</sub>O reduction to N<sub>2</sub> largely dictated the isotopic composition of measured N<sub>2</sub>O. Fungal denitrification and nitrification-derived N<sub>2</sub>O accounted for 34&#8211;42&#8201;% of total N<sub>2</sub>O emissions and had a clear effect on the measured isotopic source signatures. This study presents the suitability of on-site N<sub>2</sub>O isotopocule analysis for disentangling source and sink processes in-situ and found that at the Fendt site bacterial denitrification/nitrifier denitrification is the major source for N<sub>2</sub>O, while N<sub>2</sub>O reduction acted as a major sink.</p>