The terminal oxidase cytochrome bd-I confers carbon monoxide resistance to Escherichia coli cells

Nastasi, Martina R.; Borisov, Vitaliy B.; Forte, Elena

doi:10.1016/j.jinorgbio.2023.112341

Cited by 3 publications

(2 citation statements)

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“…For this purpose, an Agilent Cary 60 UV-Vis or a Varian Cary 300 Bio UV-Visible spectrophotometer was used. The amount of oxidase present in each strain was estimated from the dithionite-reduced-minus-ferricyanyde-oxidized difference absorption spectrum of sonicated cells using ∆ε 561-580 of 21 mM −1 cm −1 (bd-I and bd-II) [92] and 16.3 mM −1 cm −1 (bo 3 ) [88].…”

Section: Spectroscopic Measurementsmentioning

confidence: 99%

“…Of the three E. coli oxidases, cytochrome bo 3 was shown to be the least sensitive to inhibition by CO if the enzymes were purified and detergent-solubilized [87]. In contrast, according to a recent short report [88], cytochrome bd-I is more resistant to inhibition by CO than cytochrome bd-II and cytochrome bo 3 if CO is added to E. coli cell suspensions, at [O 2 ] = 150 µM. Bayly et al also studied the physiological response of Mycobacterium smegmatis to CO [83].…”

Section: Introductionmentioning

confidence: 97%

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Membrane-Bound Redox Enzyme Cytochrome bd-I Promotes Carbon Monoxide-Resistant Escherichia coli Growth and Respiration

Nastasi,

Borisov,

Forte

2024

IJMS

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The terminal oxidases of bacterial aerobic respiratory chains are redox-active electrogenic enzymes that catalyze the four-electron reduction of O2 to 2H2O taking out electrons from quinol or cytochrome c. Living bacteria often deal with carbon monoxide (CO) which can act as both a signaling molecule and a poison. Bacterial terminal oxidases contain hemes; therefore, they are potential targets for CO. However, our knowledge of this issue is limited and contradictory. Here, we investigated the effect of CO on the cell growth and aerobic respiration of three different Escherichia coli mutants, each expressing only one terminal quinol oxidase: cytochrome bd-I, cytochrome bd-II, or cytochrome bo3. We found that following the addition of CO to bd-I-only cells, a minimal effect on growth was observed, whereas the growth of both bd-II-only and bo3-only strains was severely impaired. Consistently, the degree of resistance of aerobic respiration of bd-I-only cells to CO is high, as opposed to high CO sensitivity displayed by bd-II-only and bo3-only cells consuming O2. Such a difference between the oxidases in sensitivity to CO was also observed with isolated membranes of the mutants. Accordingly, O2 consumption of wild-type cells showed relatively low CO sensitivity under conditions favoring the expression of a bd-type oxidase.

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Section: Spectroscopic Measurementsmentioning

confidence: 99%