The therapeutic effects of Lagopsis supina (Steph. ex Willd.) Ikonn.-Gal. fractions in trauma-induced blood stasis model rats

Xia, Xiaoyi; Wang, Huilei; Duan, Yelin; Yang, Li; He, Junwei

doi:10.1016/j.heliyon.2022.e11176

Cited by 4 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the protective effects of LJ against traumatic injury and diuresis have not been reported to date. According to TCM theory, traumatic BS can lead to BS, inflammation, and edema [5–8] . Therefore, in this study, for the first time, the activating blood circulation, anti‐inflammatory, and diuretic effects of LJ were simultaneously evaluated in TBSM rats.…”

Section: Methodsmentioning

confidence: 99%

“…The TBSM rat was established according to previously published method [7,8] . In brief, 16 rats in the model and LJ groups were anesthetized by intraperitoneal injection of 1 % pentobarbital sodium (40 mg/kg body weight).…”

Section: Methodsmentioning

confidence: 99%

“…Based on this, the promoting blood circulation and removing blood stasis (PBCRBS) and diuretic agents were commonly used for treating traumatic injury. On the other hand, the traumatic blood stasis model (TBSM) rat is one of blood stasis model, and widely used as a classical rodent model to evaluate the effects of PBCRBS and diuretic in vivo [5–8] . Additionally, the abnormal inflammatory response accompanied occurred by wound and blood stasis [9,10] …”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the traumatic blood stasis model (TBSM) rat is one of blood stasis model, and widely used as a classical rodent model to evaluate the effects of PBCRBS and diuretic in vivo. [5][6][7][8] Additionally, the abnormal inflammatory response accompanied occurred by wound and blood stasis. [9,10] Although good clinical applications of L. japonicus have been achieved, its effect on trauma-induced injury is currently unknown, which certain restricts its widely clinical application.…”

Section: Introductionmentioning

confidence: 99%

“…It was reported that traumatic damaged can lead to blood stasis and localized edema. [5][6][7][8] Based on this, the promoting blood circulation and removing blood stasis (PBCRBS) and diuretic agents were commonly Supporting information for this article is available on the WWW under https://doi.org/10.1002/cbdv.202201176 Part of a Special Collection on Chemical Evidence/ Interpretation to Diverse Effects of Ethnomedicines of China. used for treating traumatic injury.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Activating Blood Circulation, Anti‐Inflammatory and Diuretic Effects of Leonurus japonicus Extract on a Rat Model of Trauma Blood Stasis and Its Phytochemical Profiling

Zhang

Nie

Yang

et al. 2023

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

Leonurus japonicus Houtt. has been traditionally used to treat many ailments. This study evaluated the activating blood circulation, anti-inflammatory, and diuretic effects of L. japonicus extract (LJ) and identified its phytochemicals. In this work, the phytochemicals in LJ were identified using liquid chromatography mass spectrometry. Rats were randomly assigned to three groups (n = 8): Control group was treated with saline, while the Model group (saline) and LJ group (426 mg/kg) had induced traumatic injury. All rats were treated with once by daily oral gavage for one week. The biochemical indices and protein expression were measured. Herein, 79 constituents were identified in LJ, which were effective in elevating body weight, food consumption, water intake, and urinary excretion volume, as well as in ameliorating traumatic muscle tissues in model rats. In addition, LJ prominently decreased the contents of plasma viscosity, platelet aggregation rate, thrombin time, prothrombin time, activated partial thromboplastin time, fibrinogen, thromboxane B2 (TXB2), TXB2/6-ketoprostaglandin F1α (6-keto-PGF1α), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor 1 (PAI-1), PAI-1/tissue-type PA (t-PA), and PAI-1/u-PA, while significantly increasing antithrombin III, 6-keto-PGF1α, and t-PA contents. Furthermore, LJ notably inhibited tumor necrosis factor alpha, interleukin 6 (IL-6), IL-8, angiotensin II, antidiuretic hormone, aldosterone, aquaporin 1 (AQP1), AQP2, and AQP3 levels, and markedly elevating IL-10 and natriuretic peptide levels. Finally, LJ markedly reduced the protein expression of AQP1, AQP2, and AQP3 compared to the model group. Collectively, LJ possessed prominent activating blood circulation, antiinflammatory, and diuretic effects, thus supporting the clinical application of L. japonicus.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%