The Thermophilic Microörganisms

Gaughran, Eugene R. L.

doi:10.1128/br.11.3.189-225.1947

Cited by 64 publications

(5 citation statements)

References 74 publications

(2 reference statements)

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“…Temperature selectively affects the type of lipids, unsaturation status and the degree of membrane fluidity. This vital role played by temperature also dictates that cells cannot grow at temperatures lower than that of their lipids solidification point [21,22]. This is consistent with the first order proposal of Brock and Ingraham [23,31] about the thermal death of the organism.…”

Section: Discussionsupporting

confidence: 71%

“…Moreover, they are short chain ones which fit nicely with the degree of fluidity of their membranes and survival in such environment niche. This is supported by the work of several investigators such as BROCK (1967), [18][19][20][21][22][23]. Lauric acid or dodecanoic acid which is the most dominant fatty acids with 47.85% was found to have medical importance, especially used for treating several viral infections including (influenza; the flu); swine flu; avian flu; the common cold; fever blisters, cold sores) also Lauric acid have significant role in the treatment of genital herpes caused by herpes simplex virus (HSV), genital warts caused by human papillomavirus (HPV) and HIV/AIDS [24].…”

Section: Discussionmentioning

confidence: 63%

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Fatty acids and survival of bacteria in Hammam Pharaon springs, Egypt

Osman

Gbr²,

Abdelrazak

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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A great lack of knowledge of Hammam Pharaon's microbial community; the most famous hot spring in Sinai, Egypt, derived this work. Three different hyperthermophilic bacterial were isolated from vents in the area, where the temperature was above 80°C. Response Surface Methodology algorithm such as Central Composite Design determined the optimum cultivation conditions for these isolates. Accordingly, the best growth conditions were at 70°C and at neutral to slightly acidic pH values. The constructed phylogenetic tree built using the 16S rRNA gene sequences has shown that the isolated strains (HM101, HM102 and HM103) belong to Geobacillus, Rhodothermus and Thermus bacteria, respectively. The fatty acid profiles, an indicative of thermotolerance, dominated by the short chain Dodecanoic acid (Lauric acid; (12:0), which represented about 40% of the total fatty acid contents for each of the three isolates. The enzymatic capabilities of the three strains were determined and a-amylase was found to be the most prominent one. Our own data had led us to conclude that the length of the fatty acid chain and the degree of saturation could be species specific. Moreover, the biotechnological potentials of these local isolates could contribute to fighting viral diseases and/or improve their amylolytic activities for sugar industry; where thermotolerance is really an important factor.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 63%

Fatty acids and survival of bacteria in Hammam Pharaon springs, Egypt

Osman

Gbr²,

Abdelrazak

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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“…The mechanism of thermostability for enzymes from obligate thermophilic bacteria is of considerable contemporary interest. Several theories have been proposed in an attempt to explain thermophily, including (i) the existence of specific protective factors within the intact cell (13), (ii) a rapid resynthesis of thermally inactivated cellular components (1,6), and (iii) the possession of unusual structural features that confer thermal stability on the individual enzyme protein (13).…”

mentioning

confidence: 99%

Amino Acid Sequence Around the Catalytic Site in Glyceraldehyde-3-Phosphate Dehydrogenase from Bacillus stearothermophilus

et al. 1972

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The tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with 14 C-iodoacetate. The amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-Edman degradation. The sequence shows considerable homology with its counterparts from mesophilic sources but differs by the addition of Ala-His-His at the N -terminus and by the substitution of phenylalanine for leucine in the prototype sequence.

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“…Heat resistance of bacterial cells and spores has been related to the type and melting point of protoplasmic lipids (5). Gaughran (4) and Ingraham (7) contend that the total lipid and saturation vary little in thermophilic bacteria with changes in growth temperature.…”

mentioning

confidence: 99%

“…Gaughran (5) suggested that the melting point of lipid material may fix the minimal temperature for growth of an organism. A preponderance of branched-chain fatty acids at the lower temperature suggests the presence of lipid complexes with diminished melting points, since a methyl group in a fatty acid will lower the melting point (6).…”

mentioning

confidence: 99%