The Thiobacilli, 12

Vishniac, Wolf; Santer, Melvin

doi:10.1128/mmbr.21.3.195-213.1957

Cited by 594 publications

(249 citation statements)

References 7 publications

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“…Starter cultures were obtained from a single colony and were incubated in LB with the appropriate antibiotic compound until OD 600 50.6 and subsequently incubated minimal medium until OD 600 50.6. Pure cultures of bacteria were cultured in minimal medium (MM) based on a minimal salt solution (Vishniac and Santer, 1957 Continuous culture of the bacterial strains in continuous stirred tank reactors (CSTR) was essentially as described previously (Felgate et al, 2012). Briefly, the culture volume occupied 1.5 L out of 2.5 L total vessels capacity (BioFlo 310, New Brunswick Scientific, USA).…”

Section: Bacterial Strains and Culture Techniquesmentioning

confidence: 99%

Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification

Giannopoulos

Sullivan

Hartop

et al. 2017

Environmental Microbiology

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Summary Bacterial denitrification is a respiratory process that is a major source and sink of the potent greenhouse gas nitrous oxide. Many denitrifying bacteria can adjust to life in both oxic and anoxic environments through differential expression of their respiromes in response to environmental signals such as oxygen, nitrate and nitric oxide. We used steady‐state oxic and anoxic chemostat cultures to demonstrate that the switch from aerobic to anaerobic metabolism is brought about by changes in the levels of expression of relatively few genes, but this is sufficient to adjust the configuration of the respirome to allow the organism to efficiently respire nitrate without the significant release of intermediates, such as nitrous oxide. The regulation of the denitrification respirome in strains deficient in the transcription factors FnrP, Nnr and NarR was explored and revealed that these have both inducer and repressor activities, possibly due to competitive binding at similar DNA binding sites. This may contribute to the fine tuning of expression of the denitrification respirome and so adds to the understanding of the regulation of nitrous oxide emission by denitrifying bacteria in response to different environmental signals.

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Section: Bacterial Strains and Culture Techniquesmentioning

confidence: 99%

Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification

Giannopoulos

Sullivan

Hartop

et al. 2017

Environmental Microbiology

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“…Our minimal medium is based upon one used previously to culture Hyphomicrobium spp. (Attwood and Harder 1972) and contains a trace metal solution based on Vishniac (Vishniac and Santer 1957). This medium recipe has been the standard for growth of Methylobacterium for over two decades (Fulton et al 1984); however, the trace metal solution of this standard laboratory recipe that has been used with Methylobacterium is 50-fold more dilute than the originally reported Vishniac recipe in 1957 and the pH of this component is now 5.0 instead of 6.0.…”

Section: Populationsmentioning

confidence: 99%

ASYMMETRIC, BIMODAL TRADE-OFFS DURING ADAPTATION OFMETHYLOBACTERIUMTO DISTINCT GROWTH SUBSTRATES

2009

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“…A standard mineral salts medium was used for enrichment, isolation and growth experiments and contained (per litre distilled water): 8.9 mM K 2 HPO 4 , 6.2 mM NaH 2 PO 4 , 9.3 mM NH 4 Cl, 0.4 mM MgSO 4 , 1 mL of a trace solution described by Vishniac & Santer (1957).…”

Section: Methodsmentioning

confidence: 99%

Anaerobic degradation of long-chain alkylamines by a denitrifying Pseudomonas stutzeri

Nguyen

Ginkel

Plugge

2008

FEMS Microbiology Ecology

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The anaerobic degradation of tetradecylamine and other long-chain alkylamines by a newly isolated denitrifying bacterium was studied. Strain ZN6 was isolated from a mixture of soil and active sludge and was identified as representing Pseudomonas stutzeri, based on partial 16S rRNA gene sequence analysis. Strain ZN6 was a mesophilic, motile, Gram-negative rod-shaped bacterium and was able to grow on a variety of compounds including even-numbered primary fatty amines with alkyl chains ranging from C(4) to C(18) coupled to nitrate reduction. Alkylamines were used as sole carbon, energy and nitrogen source and were completely mineralized. Nitrate was dissimilated by ZN6 to nitrite. When strain ZN6 was grown under nitrate limitation, nitrite was slowly dissimilated further. When cocultivated with the complete denitrifier Castellaniella defragens ZN3, anaerobic degradation under denitrifying of alkylamines by strain ZN6 was slightly faster. Strain ZN3 is a complete denitrifier, unable to convert tetradecylamine, and was copurified from the same enrichment culture as strain ZN6. The proposed pathway for the degradation of alkylamines in strain ZN6 starts with C-N cleavages to alkanals and further oxidation to the corresponding fatty acids.

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The Thiobacilli, 12

Cited by 594 publications

References 7 publications

Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification

Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification

ASYMMETRIC, BIMODAL TRADE-OFFS DURING ADAPTATION OFMETHYLOBACTERIUMTO DISTINCT GROWTH SUBSTRATES

Anaerobic degradation of long-chain alkylamines by a denitrifying Pseudomonas stutzeri

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