The threshold at which substrate nanogroove dimensions may influence fibroblast alignment and adhesion

Loesberg, W. A.; Riet, Joost te; Delft, F.C.M.J.M. van; Schön, Peter; Figdor, Carl G.; Speller, S.; Loon, Jack J. W. A. van; Walboomers, X. Frank; Jansen, John A.

doi:10.1016/j.biomaterials.2007.05.030

Cited by 318 publications

(255 citation statements)

References 27 publications

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“…Moreover, they observed that focal adhesions and actin fibres co-align with the ridge direction, eventually leading to cell alignment (a phenomenon usually called contact guidance). Along this line, Loseberg et al [65] and Lamers et al…”

Section: Topo-cue-mediated Crosstalkmentioning

confidence: 92%

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Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

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The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional biological tissues, advanced cell culture systems, single-cell diagnosis as well as for cell sorting and differentiation. It is well established that crosstalk at the cell–material interface occurs and this has a profound influence on cell behaviour. However, the complete deciphering of the cell–material communication code is still far away. A variety of material surface properties have been reported to affect the strength and the nature of the cell–material interactions, including biological cues, topography and mechanical properties. Novel experimental evidence bears out the hypothesis that these three different signals participate in the same material–cytoskeleton crosstalk pathway via adhesion plaque formation dynamics. In this review, we present the relevant findings on material-induced cell response along with the description of cell behaviour when exposed to arrays of signals—biochemical, topographical and mechanical. Finally, with the aid of literature data, we attempt to draw unifying elements of the material–cytoskeleton–cell fate chain.

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Section: Topo-cue-mediated Crosstalkmentioning

confidence: 92%

“…focal complexes and adhesions. Several works have reported that 70 nm seems to be a limiting inter-feature dimension under which topographic signals begin to be less effective [65,66]. Within such a dimensional range, i.e.…”

Section: Topo-cue-mediated Crosstalkmentioning

confidence: 98%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

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“…Despite the large numbers of works that have been developed so far on cell-topography interactions, a general consensus on what settings of topographic features elicit specific cell functions has not been reached yet. For example, while certain combinations of topographies promote cell alignment and migration, others report different trends [12,13].…”

Section: Introductionmentioning

confidence: 99%

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Ventre

Natale

Rianna

et al. 2014

J. R. Soc. Interface.

110

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Topographic patterns are known to affect cellular processes such as adhesion, migration and differentiation. However, the optimal way to deliver topographic signals to provide cells with precise instructions has not been defined yet. In this work, we hypothesize that topographic patterns may be able to control the sensing and adhesion machinery of cells when their interval features are tuned on the characteristic lengths of filopodial probing and focal adhesions (FAs). Features separated by distance beyond the length of filopodia cannot be readily perceived; therefore, the formation of new adhesions is discouraged. If, however, topographic features are separated by a distance within the reach of filopodia extension, cells can establish contact between adjacent topographic islands. In the latter case, cell adhesion and polarization rely upon the growth of FAs occurring on a specific length scale that depends on the chemical properties of the surface. Topographic patterns and chemical properties may interfere with the growth of FAs, thus making adhesions unstable. To test this hypothesis, we fabricated different micropatterned surfaces displaying feature dimensions and adhesive properties able to interfere with the filopodial sensing and the adhesion maturation, selectively. Our data demonstrate that it is possible to exert a potent control on cell adhesion, elongation and migration by tuning topographic features' dimensions and surface chemistry.

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“…Cellular orientation is also influenced by topographical features, a phenomenon known as contact guidance (CG). For example, experimental studies have demonstrated that, when seeded on substrates having nano/microgrooves, cells tend to orient themselves along the direction of these patterns (8)(9)(10)(11)(12)(13)(14)(15)(16). A similar behavior can be observed when cells are cultured on substrates having linear patterns created with microcontact printing (17)(18)(19)(20), or on top of microposts with an ellipsoidal base (21).…”

Section: Introductionmentioning

confidence: 84%

Prediction of Cell Alignment on Cyclically Strained Grooved Substrates

Ristori

Vigliotti

Baaijens

et al. 2016

Biophysical Journal

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Cells respond to both mechanical and topographical stimuli by reorienting and reorganizing their cytoskeleton. Under certain conditions, such as for cells on cyclically stretched grooved substrates, the effects of these stimuli can be antagonistic. The biophysical processes that lead to the cellular reorientation resulting from such a competition are not clear yet. In this study, we hypothesized that mechanical cues and the diffusion of the intracellular signal produced by focal adhesions are determinants of the final cellular alignment. This hypothesis was investigated by means of a computational model, with the aim to simulate the (re)orientation of cells cultured on cyclically stretched grooved substrates. The computational results qualitatively agree with previous experimental studies, thereby supporting our hypothesis. Furthermore, cellular behavior resulting from experimental conditions different from the ones reported in the literature was simulated, which can contribute to the development of new experimental designs.

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The threshold at which substrate nanogroove dimensions may influence fibroblast alignment and adhesion

Cited by 318 publications

References 27 publications

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Prediction of Cell Alignment on Cyclically Strained Grooved Substrates

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