Background: Previous studies show that people with lower limb amputation (LLA) have a sedentary lifestyle, reduced walking capacity, and low cardiorespiratory fitness (VO 2 peak). There is, however, no knowledge on the relationship between cardiorespiratory fitness and objectively measured level of physical activity in daily life. Objectives: To investigate the relationship between upper-body VO 2 peak, physical activity levels, and walking capacity in persons with LLA.Study design: Correlational and descriptive study. Methods: Fourteen participants with LLA performed an assessment of VO 2 peak on an arm-crank ergometer and walking capacity (preferred walking speed and 2-minute walking test). Level of physical activity was measured over 7 days with a step activity monitor (number of steps; sedentary time; and proportion of low-intensity, moderate-intensity, high-intensity, and peak-intensity activity level). Results: VO 2 peak correlated significantly with number of steps per day (r 5 0.696, p 5 0.006), sedentary time (r 5 20.618, p 5 0.019), high-intensity activity level (r 5 0.769, p 5 0.001), and peak-intensity activity level (r 5 0.674, p 5 0.008). After correcting for age, correlations were still large and significant. Large correlations were also found between VO 2 peak, preferred walking speed (r 5 0.586, p 5 0.027), and 2-minute walking test (r 5 0.649, p 5 0.012). Conclusions: We provide the first evidence of the strong relationships between upper-body VO 2 peak, sedentary behavior, highintensity activity level, and walking capacity in persons with LLA. Further research is needed to investigate the potential effect of upperbody cardiorespiratory fitness on the level of activity in daily life, or vice versa.