The time-course of the induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1

Abstract

Recombinant human osteogenic protein-1 (hOP-1, BMP-7) induces cartilage and bone formation when implanted in extra- and intraskeletal sites in vivo. rOP-1 also preserves pulp vitality and stimulates reparative dentine formation when placed on partially amputated vital dental pulp tissue. The amount of dentine formed in 6 weeks was earlier found to be proportional to the total amount of rOP-1/carrier placed on the pulp and the capacity of the pulp to respond to rOP-1 appeared to be independent of the amount of coronal pulp removed. This reparative dentine was not completely mineralized after 6 weeks healing. Experiments were now made to determine the capacity of hOP-1 to preserve the vitality of and induce reparative dentine in vital radicular pulps. The extent of tissue mineralization present after 1, 2, 4 and 6 months' healing time in permanent monkey teeth was assessed. Radicular pulp vitality was maintained, reparative dentine formed, and mineralization was nearly 75% complete after 1 month and more than 95% after 4 months. The effects of irrigating the exposed pulps with EDTA, sodium hypochlorite or saline were also compared. Significantly more sodium hypochlorite-treated pulps became non-vital and the root canals of all the non-vital teeth contained bacteria at the time of sacrifice.

Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf11)

“…BMPs have actions beyond bone in neural, renal and cardiac development. 1,2 BMPs also play a role in differentiation of dentin [3][4][5][6][7][8] in teeth. The recent progress in molecular developmental biology permits the delivery of BMPs by gene therapy using optimal delivery.…”

Current status of direct pulp-capping materials for permanent teeth

“…However, chronic inflammation and a lack of pulp healing were observed, with no dentin bridge formation. It was later reported that eugenol is highly toxic, and zinc oxide eugenol resulted in high interfacial leakage [19][20][21][22] . In the 1970s, glucocorticoids combined with antibiotics were frequently used in an attempt to control pulpal pain and suppress pulpal inflammation 19,20,23) .…”

Vi̇tal Pulpa Tedavi̇leri̇nde Güncel Yaklaşimlar

“…92 bağ dokusunun daha sonra mineralize olarak oluş turduğu tamir dentinin, pulpa dokusuna yüzeyel olarak şekillendiği ve derin pulpa dokusunu harcamaksızın pulpanın sağlıklı bir şekilde korunmasını sağladığı gösterilmiştir. 96 TGF-beta ailesi üyelerinden olan Bone Morphgenic Protein'lerden BMP-2, BMP-4 ve BMP-7 (OP-1) gibi proteinlerin de osteodentin ve ardından tübüler tamir dentin formasyonunu indüklediği bildirilmiştir. 97 Büyüme faktörleri ailesi -İnsülin benzeri büyü-me faktörleri‖ (IGF) üyelerinden IGF-I ile gerçekleş-tirilen pulpa kaplaması deneylerinde; pulpa ekspozunu tam olarak kapatan bir dentin köprüsünün ve arada tübüler tamir dentin formasyonunun oluştuğu gösterilmiştir.…”