The time-dependent expression of keratins 5 and 13 during the reepithelialization of human skin wounds

Betz, P.; Nerlich, Andreas G.; Tübel, Jutta; Penning, R.; Eisenmenger, Wolfgang

doi:10.1007/bf01642799

Cited by 14 publications

(9 citation statements)

References 16 publications

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“…In our series, an enhanced expression of Ki 67 antigen in keratinocytes covering the previous epithelial lesion could not be established. This supports the hypothesis that keratinocyte migration which starts from areas of undamaged skin with an increased proliferation activity but not local proliferation plays the major role in reepithelialization [2,17]. In the epidermal layers adjacent to the wound area, however, an increased number of Ki 67 positive basal cells occurred in the most cases and a wave-like course in the numbers of proliferating basal cells with advancing distance from the wound margins could be observed according to experimental results.…”

Section: Discussionsupporting

confidence: 86%

The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds

Betz

Nerlich²,

Wilske

et al. 1993

Int J Leg Med

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Summary.A total of 77 human skin wounds with a postinfliction interval between 3 h and 7 months were investigated and the proliferation marker antigen Ki 67 was visualized in paraffin sections using a specific monoclonal antibody (MIB). The re-built epidermal layer covering the former lesional area showed only a few basal cells positively staining for Ki 67 antigen. No enhanced reactivity was found when compared to uninjured skin. In basal cells of the epidermis adjacent to the wound area, however, varying numbers of positive cells occurred, but no information useful for a reliable time estimation of skin wounds could be obtained due to the considerable variability in the number of Ki 67 positive epidermal basal cells found in non-damaged skin. Fibroblastic cells in the wound area revealed an increased number of Ki 67-positive sites which could first be detected in a 1.5-day-old skin lesion. Positive results could be obtained in every specimen investigated after a post-infliction interval of 6 days up to 1.5 months. Only the scar tissue of the oldest wound examined (wound age 7 months) revealed no increase in the number of positively staining fibroblasts. Therefore, positive results indicate a wound age of at least approximately 1.5 days and the lack of an increased number of positive fibroblastic cells in a sufficient number of specimens indicates at a wound age of less than 6 days, but cannot totally exclude longer postinfliction intervals.

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Section: Discussionsupporting

confidence: 86%

The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds

Betz

Nerlich²,

Wilske

et al. 1993

Int J Leg Med

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“…This correlated to a nearly 13-fold reduction in slope for the K10 H-score across the epithelial tongue (Fig 5, third row). Defects in reepithelialization are associated with chronic wounds and malignant transformation in which reciprocal expression of K10 and K13 are often observed [29, 30]. Again, we observed that Gα 11 KO/Gα q -WT control animals had a steady, nearly linear increase in K13 H-score across the epithelial tongue that was abrogated in the Gα 11 KO/Gα q -eKO animals.…”

Section: Resultssupporting

confidence: 52%

Epidermal loss of Gαq confers a migratory and differentiation defect in keratinocytes

et al. 2017

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G-protein coupled receptors (GPCRs), which activate heterotrimeric G proteins, are an essential class of transmembrane receptors that are responsible for a myriad of signaling events in normal and pathologic conditions. Two members of the G protein family, Gαq and Gα11, activate one of the main GPCR pathways and function as oncogenes by integrating mitogen-stimulated signaling cascades that are active under malignant conditions. Recently, it has been shown that targeted deletion of Gα11 and Gαq from endothelial cells impairs the Rho-mediated formation of focal adherens junctions, suggesting that Gα11/q signaling may also play a significant role in cytoskeletal-mediated cellular responses in epithelial cells. Indeed, combined deletion of Gα11 and Gαq confers a significant migratory defect in keratinocytes that delays cutaneous wound healing in an in vivo setting. This delay can be attributed to a defect during the reepithelialization phase due to significantly attenuated migratory capacity of Gαq-null keratinocytes under combined Gα11 deficiency. In fact, cells lacking Gα11/q demonstrate a severely reduced ability to respond to mitogenic and migratory signals in the microenvironment, leading to inappropriate and premature terminal differentiation. These results suggest that Gα11/q signaling pathways may be critical for integrating mitogenic signals and cytoskeletal function to achieve normal physiological responses. Emergence of a malignant phenotype may therefore arise from both under- and overexpression of Gα11/q signaling, implicating its upstream regulation as a potential therapeutic target in a host of pathologic conditions.

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“…The change of CK expression, therefore, in the epidermis of fairly remote areas from wound edges does not seem to be caused by a hyperproliferative state of keratinocytes. Betz et al (1993) have reported that the basal cell layer of the new epithelium first showed a complete positive staining for CK 5 after approximately 13 days survival time. The present study, however, revealed that positive staining for CK 5 was noted in the basal cell layer of the epidermal tongue 1 day after wounding, although the staining intensity was decreased.…”

Section: Discussionmentioning

confidence: 97%

Changes in cytokeratin expression in epidermal keratinocytes during wound healing

Watanabe

Osumi

Ohnishi

et al. 1995

Histochem Cell Biol

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In order to investigate the re-epithelialization process during wound healing, the hair on the back of guinea pigs was shaved and then excisional wounds were made through the entire thickness of the skin. Histological changes were observed and changes in the expression of different cytokeratin polypeptides were examined using an immunohistochemical technique. Immunohistochemical study revealed that the proliferating and migrating keratinocytes expressed the same cytokeratins as the basal cells of normal epidermis. In addition, the entire epidermis of fairly remote areas from the edges of the wound where no thickening was observed showed a temporarily abnormal staining pattern. The suprabasal cells in the regenerating epidermis temporarily expressed cytokeratins not only specific for suprabasal cells but also specific for basal cells. The cytokeratins expressed in normal basal keratinocytes were also present in the thickened granular layers. These data indicate that the expression of cytokeratins in the epidermal keratinocytes (even in fairly remote areas from the wound edges) changes during wound healing, that the origin of the migrating keratinocytes from the remaining epidermis seems to be the basal cells in the epidermis, and that the appearance of keratohyalin granules is not related to changes in cytokeratin expression.

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The time-dependent expression of keratins 5 and 13 during the reepithelialization of human skin wounds

Cited by 14 publications

References 16 publications

The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds

The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds

Epidermal loss of Gαq confers a migratory and differentiation defect in keratinocytes

Changes in cytokeratin expression in epidermal keratinocytes during wound healing

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