The tissue-specific extinguisher locus TSE1 encodes a regulatory subunit of cAMP-Dependent protein kinase

Comparative PRKAR1A genotype–phenotype analyses in humans with Carney complex and prkar1a haploinsufficient mice

“…Moreover, others have invoked a relationship of vascular proliferative lesions to myxomas because myxoma cells can express ''endothelial'' molecular markers (29)(30)(31). CNC's relationship to hepatocellular carcinoma is less clear; PRKAR1␣ was initially identified as a locus that repressed transcription specifically in hepatocellular carcinoma cells (32,33). Disparities between prkar1a ϩ/Ϫ mice and CNC may reflect interspecies differences in predilection for different tumor types; these are common, e.g., between human neurofibromatosis and Nf1 ϩ/Ϫ mice (34,35).…”

Transcriptional control of genes that regulate glycolysis and gluconeogenesis in adult liver

“…The Tse-J locus is responsible for the extinction of the PEPCK, tyrosine aminotransferase and aldolase B genes in hybrid cells [167]. The product of the Tse-J locus is the regulatory subunit RIa of PKA [168,169]. Rla is expressed in many cell types but at a 50-fold lower level in the liver.…”

A Developmentally Regulated Aconitase Related to Iron-regulatory Protein-1 Is Localized in the Cytoplasm and in the Mitochondrion of Trypanosoma brucei

“…The hybridization and washing temperatures were adjusted to 5°C below the calculated melting temperature of specific DNA-DNA hybrids for high stringency conditions and to 40°C below the calculated melting 1 The abbreviations used are: IRP, iron-regulatory protein; IPTG, isopropyl-1-thio-␤-D-galactopyranoside; IRE, iron-responsive element; kb, kilobase pairs; MES, 2-(N-morpholino)-ethanesulfonic acid; NBT, nitro blue tetrazolium; PCF, procyclic forms; PCR, polymerase chain reaction; SS, short stumpy forms; UTR, untranslated region; bp, base pair; DTT, dithiothreitol; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; RACE, rapid amplification of cDNA ends; NTA, nitrilotriacetic acid. temperature for low stringency conditions, using the buffers described in Boshart et al (30). A TbACO-specific probe of defined length (nucleotides 1778 -2088 of Tbaco1.1) was generated by primer extension (31) with polylinker-specific primers (SK and KS) using as template an EagI-and ApaI-cut pBluescript plasmid containing the 311-bp PCR fragment.…”