The titers and the clearance of ecdysteroids from the blood of last instar larvae ofGalleria mellonella L.

Rohner, Renate M.; Meyer, Dietrich

doi:10.1007/bf01969616

Cited by 8 publications

(2 citation statements)

References 13 publications

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“…5). Unlike in the cultured glands, both mRNAs disappeared soon after the hormone administration, probably due to the rapid clearance of 20E from the hemolymph (Rohner and Meyer 1980). E75B was induced to barely detectable levels (data not shown).…”

Section: Induction Of E75 and Ghr3 Mrnas By 20ementioning

confidence: 49%

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Expression of ecdysteroid-regulated transcripts in the silk gland of the wax moth, Galleria mellonella

Jindra

Riddiford

1996

Development Genes and Evolution

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Expression of six different transcripts encoding members of the nuclear receptor superfamily was studied in whole silk glands of the wax moth, Galleria mellonella, during development and in response to 20-hydroxyecdysone (20E) and juvenile hormone (JH II) in vitro. Nucleic acid probes specific to Galleria homologs of the Drosophila E75 early gene and the delayed early gene DHR3 were used to follow mRNAs encoding isoforms A and B of the E75 protein and GHR3 protein, respectively. Among these mRNAs, E75A mRNA appeared first during the larval molt and was most rapidly induced by 20E, while E75B and GHR3 mRNAs appeared with a 2 h delay. Unlike E75B mRNA, GHR3 mRNA required protein synthesis for its full induction. The 20E-induced levels of E75A mRNA were increased one and a half-fold within 1 h in the presence of 10-100 ng/ml JH II in cultured silk glands. Antisense cRNA probes for the Manduca sexta ecdysone receptor isoforms EcR-A and EcR-B1 detected transcripts that were directly induced by 20E in cultured silk glands. The levels of the putative EcR-A mRNA were high relative to those of EcR-B1 mRNA in silk glands at molting and metamorphosis. An antisense cRNA probe for the Manduca Ultraspiracle homolog detected a 4 kb mRNA that was most abundant around the time of larval ecdysis but was not induced by 20E in vitro.

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Section: Induction Of E75 and Ghr3 Mrnas By 20ementioning

confidence: 49%

“…Last instar larvae were ligatured across the metathorax, decapitated, and left at 30°C for 3 days to exclude interference from endogenous hormones (Rohner and Meyer 1980). These isolated abdomens were then either injected with 20-hydroxyecdysone (20E; Sigma; Jindra et al 1994a) or used as silk gland donors for in vitro studies.…”

Section: Animalsmentioning

confidence: 99%

Expression of ecdysteroid-regulated transcripts in the silk gland of the wax moth, Galleria mellonella

Jindra

Riddiford

1996

Development Genes and Evolution

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Brain factor fromGalleria mellonella (Lepidoptera) stimulating silk gland activity

1992

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Brain extracts from day 1-4 last instar larvae of Galleria mellonella (Lepidoptera) stimulate RNA synthesis in cultured silk glands from day 3 last instar larvae. When the fibroin-synthesizing posterior parts of silk glands were incubated for 3 h in vitro in the presence of brain extract (0.1 brain equivalent), [3H]-uridine incorporation into RNA was stimulated more than twofold. The stimulating effect of brain extract showed a dose response relationship. It is suggested that the heat-resistant and protease-sensitive brain factor is a peptide.

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Ecdysone metabolism in adult crickets, Gryllus bimaculatus

Bulenda

Stecher

Freunek

et al. 1986

Insect Biochemistry

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The titers and the clearance of ecdysteroids from the blood of last instar larvae ofGalleria mellonella L.

Cited by 8 publications

References 13 publications

Expression of ecdysteroid-regulated transcripts in the silk gland of the wax moth, Galleria mellonella

Expression of ecdysteroid-regulated transcripts in the silk gland of the wax moth, Galleria mellonella

Brain factor fromGalleria mellonella (Lepidoptera) stimulating silk gland activity

Ecdysone metabolism in adult crickets, Gryllus bimaculatus

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