The Titration Curve of Glutathione

Pirie, N. W.; Pinhey, Kathleen Godwin

doi:10.1016/s0021-9258(18)77063-4

Cited by 91 publications

(30 citation statements)

References 9 publications

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“…Pirie and Pinhey (23) and Harris (19) have found evidence of a combination at alkaline reactions between HCHO and the -SH group of cysteine which results in a perceptible weakening of the acidity of the -SH group and a shift of the pK to increased basicity.…”

Section: Discussionmentioning

confidence: 99%

The Modification of Antibodies by Formaldehyde

Mudd

Joffe

1933

Journal of General Physiology

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Interaction of antigen and antibody forms upon the antigen surfacO a more or less complete film of antibody-globulin held by specific stereochemical forces. The various serological reactions are consequent upon the properties of this surface deposit and the special environing conditions. Thus the antibody-globulin surface deposit under ordinary conditions of test has a surface potential difference below and cohesiveness above the mean critical values for stability, (agglutination occurs (1, 2)); upon the antibody-globulin surface polymorphonuclear and mononudear phagocytes tend to spread, (phagocytosis occurs (3, 4, 5)); and the antigen-antibody complex is a good adsorbent for the serum components known as complement, (complement-fixation occurs (6, 7)).Study of the conditions of formation and the physical-chemical attributes of these antibody-globulin films is then clearly of strategic value in gaining further understanding of the serological reactions. The property of the surface film susceptible of most accurate measurement is the electrokinetic P.D. (zeta potential). In undertaking certain studies on this factor we have been aided by possession of strains of bacteria which themselves have only minimal zeta potentials over a wide range of pH, and therefore afford a minimal risk of confusing the zeta potentials of the films with those of the bacterial surfaces (2,8).In the present study antibodies before and after combination with antigen have been treated with formaldehyde, and the effects upon 1 The term "antigen surface" is used without intended implication as to whether the antigen is in molecular dispersion, in micellae, or forms part of a cell surface, and as to whether or not the reaction is stoich~ometric.

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Section: Discussionmentioning

confidence: 99%

The Modification of Antibodies by Formaldehyde

Mudd

Joffe

1933

Journal of General Physiology

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“…Based on previous work by Briñas et al, 29 Au(I) ions were bridged by ligands and formed a polymeric complex. Their hydrodynamic radii were related to pH values, which were determined by the ionization states of GSH 30 at different pH values. In the pH range of 2.12(pK1) to 3.53(pK2), the net charge of GSH is zero.…”

Section: Au(i) Intermediate: a Key Factor In The Bio-reduction Proces...mentioning

confidence: 99%

Intermediate-dominated controllable biomimetic synthesis of gold nanoparticles in a quasi-biological system

et al. 2010

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A new biomimetic strategy of creating a quasi-biological system (an aqueous solution containing electrolytes, peptide, enzyme and coenzyme) for the preparation of gold nanoparticles with uniform and tunable sizes has been put forward and validated, adopting environmentally-friendly reducing agents and a biocompatible capping ligand in aqueous solution at room temperature. The biomimetic synthetic route has the characteristics for good stability of the resulting AuNPs capped with glutathione via strong Au-S bond in aqueous solution, an appropriate composition of the intermediate with a redox potential favorable for the biomimetic reduction under mild conditions, suitable pH values to adjust the rate of the reduction, and the addition of enzyme catalyzing the reduction. By only adjusting the concentration of the reducing agent NADPH, a series of AuNPs with narrow size-distribution could be controllably synthesized. This method of rational utilization of biological processes could provide a new way for the sustainable development of nanotechnology.

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“…It is known that GSH has four ionization forms with pK 1 , pK 2 , pK 3 , and pK 4 values of 2.12 (-COOH of the glutamic residue), 3.53 (-COOH of the glycine residue), 8.66 (-NH 3 + ), and 9.62 (-SH), respectively. 34 Herein, the hydrogel is obtained at pH 5.2, from which it can be deduced that GSH molecules in the gel are in the zwitterionic state with both dissociated carboxyl groups.…”

mentioning

confidence: 99%

Silver(i)–glutathione biocoordination polymer hydrogel: effective antibacterial activity and improved cytocompatibility

Liu

et al. 2011

J. Mater. Chem.

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The Titration Curve of Glutathione

Cited by 91 publications

References 9 publications

The Modification of Antibodies by Formaldehyde

The Modification of Antibodies by Formaldehyde

Intermediate-dominated controllable biomimetic synthesis of gold nanoparticles in a quasi-biological system

Silver(i)–glutathione biocoordination polymer hydrogel: effective antibacterial activity and improved cytocompatibility

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