The TNF-α, P53 Protein Response and Lung Respiratory Changes Related to Exercise, Chronic Hypoxia and Adiantum capillus-Veneris Supplementation

Abstract

Introduction: Evidence suggests that hypoxia and high-intensity exercise training can increase apoptosis of lung cells and Adiantum capillus-veneris (Ac-v) extract can have anti-apoptotic effects. Thus, the aim of the present study was to investigate the effect of chronic hypoxia and the (Ac-v) extraction as a supplement on TNF-a and P53 protein expression as well as the respiratory surface. Material and methods: 24 healthy Wistar rats (age = 4 weeks, weight = 72 = 9 gr) were trained using interval training for 6 weeks followed by a 3-week stay in hypoxia conditions. Half of the hypoxia samples received 500 ml/gr/per body weight daily (Ac-v) within 3 weeks of hypoxia. At the end, the lung tissue was removed for histological and immunohistological analysis. Results: After 3 weeks of hypoxia exposure following 6 weeks of exercise, expression of P53 and TNF-a increased and the respiratory surface decreased (p ≤ 0.05). After 3 weeks of taking the Ac-v extract during hypoxia exposure, reduced P53 and TNF-a expression and the increased respiratory surface were observed (p ≤ 0.05). Conclusions: Chronic hypoxia may be considered as a strong stimulus leading to the expression of proteins involved in apoptosis and tissue disruption. However, our findings suggest that the antioxidative properties of Ac-v extract could decrease the destructive structural and molecular events that happen along with hypoxia exposure or intense exercise training.

Managing MMP-2, MMP-9, VEGFR-2, TGFβ-1, and TIMP-1 in NNK-induced lung carcinoma by nonchemical interventions in female rats

“…The phosphorylation of mutant p53 at Ser6/Ser9 by Ras/MAPK signaling is a crucial hallmark for TGF-β to switch to become a tumor promoter. N.sativa [43] and exercise training [44] are known as stimulators of p53 which can indirectly affect the expression behavior of TGFβ-1. In the present study, treatment with N.sativa and exercise training (E and NS+E groups), remarkably increased the gene expression of TGFβ-1 in healthy rats (E and NS+E) versus control subjects ( Fig.…”

Effect of Silymarin Supplementation in Lung and Liver Histological Modifications during Exercise Training in a Rodent Model

“…The study with the Andiantum capillus-veneris (Ac-v) extract, a species for which antiapoptotic activity has been described, demonstrated that in treated rats with 500 mL/g/kg body weight (bw)/day for 3 weeks exposed to hypoxic conditions, followed by 6 weeks of an interval training protocol, the expression of p53 protein and of TNF-α was reduced inversely, while the respiratory surface increased. These apoptotic indicators were altered under hypoxic conditions and treatment with the Ac-v extract acted against damaging events [ 43 ]. In relation to silymarin and the protective effects on lung tissue, in a model of acute lung damage by aspiration with hydrochloric acid (HCl) (1.2 mL/kg), oral treatment with silymarin at 200 mg/kg for 7 days reduced the indicators of histopathological damage caused in the lung by exposure to HCl.…”

Physical Activity and Natural Products and Minerals in the SARS-CoV-2 Pandemic: An Update

“…In another study by Yadegari et al, the effect of chronic hypoxia and Adiantum capillusveneris supplements on protein response of P53, TNF-a and exercise -related respiratory changes were investigated and was shown that 3 weeks of exposure to hypoxia, after 6 weeks of exercise, increased P53 and TNF-a and decreased respiratory rate. After 3 weeks of taking Ac-v extract during exposure to hypoxia, the levels of P53 and TNF-a decreased and the level of respiration increased (95).…”

“…In a study of plant composition in northern Iran based on phytochemical tests, it was found that this plant contains tannins, flavonoids, terpenoids and unsaturated sterols (95). Separation of active ingredients was based on the presence of terpenoids and unsaturated sterols.…”

“…2) a derivative of isoburonol with an epoxy structure with a closed formula (C29H48O) and a molecular mass of 412. terletala terimet (3) D methyl terephthalate. With closed formula (C10H10O4) and molecular mass 194 (95,96). The extract of this plant is used as an herbal medicine and emollient to soothe and strengthen the breast.…”