The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation

Gallie, Daniel; Tanguay, Robert L.; Leathers, Valerie

doi:10.1016/0378-1119(95)00521-7

Cited by 106 publications

(91 citation statements)

References 14 publications

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“…3). This result agrees with previous experiments, also carried out with methylated and unmethylated cap, which showed that potyvirus translation initiation was cap-independent (Carrington & Freed, 1990 ;Riechmann et al, 1991 ;Gallie et al, 1995 (Fig. 4), thus confirming that neither primary nor secondary structures are necessary for efficient translation initiation of PPV genomic RNA.…”

Section: Translational Efficiency Of Ppv 5h Ncr Mutant Mrnas In Protosupporting

confidence: 92%

“…In the case of potyviruses, such a synergism has been shown between the 5h NCR and the poly(A) tail of the TEV mRNA (Gallie et al, 1995). Although the mRNAs used in the transient expression experiments included the 5h NCR of PPV and were polyadenylated, we compared the translational efficiency of the RNA transcribed from pGG5GUS (tGG5GUS), which was synthesized with unmethylated cap and contained the last 94 nt of the 221 nt long 3h NCR of PPV, with that of tGG53GUS, a transcript also synthesized with unmethylated cap, that contained the entire 3h NCR of PPV.…”

Section: Translational Efficiency Of Ppv 5h Ncr Mutant Mrnas In Protomentioning

confidence: 94%

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Cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA.

Simón-Buela

Guo

García

1997

Journal of General Virology

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Section: Translational Efficiency Of Ppv 5h Ncr Mutant Mrnas In Protosupporting

confidence: 92%

Section: Translational Efficiency Of Ppv 5h Ncr Mutant Mrnas In Protomentioning

confidence: 94%

Cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA.

Simón-Buela

Guo

García

1997

Journal of General Virology

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Section: Introductionsupporting

confidence: 81%

“…Disruption of PABP-eIF4G or PABP-poly(A) tail interactions on IRES-driven reporter mRNAs leads to reduced rates of translation (Bradrick et al, 2007;Khaleghpour et al, 2001;Michel et al, 2000;Svitkin et al, 2001). Similar results were also obtained using IRES derived from the Tobacco etch virus (Gallie, 2001;Gallie et al, 1995 , 1997) and disruption of the interaction leads to resistance (Léonard et al, 2000). Requirement for eIF4E/4G or eIF(iso)4E/4G isomers depends on the virus being studied, as A. thaliana knockout plants for eIF(iso)4E or eIF(iso)4G1/2 genes are resistant to TuMV, lettuce mosaic virus and plum pox virus infection, while disruption of eIF4E1 or eIF4G results in resistance to clover yellow vein virus (Decroocq et al, 2006; Duprat et al, 2002;Lellis et al, 2002;Nicaise et al, 2007;Sato et al, 2005).…”

Section: Introductionsupporting

confidence: 56%

Arabidopsis thaliana class II poly(A)-binding proteins are required for efficient multiplication of turnip mosaic virus

Dufresne

Ubalijoro

Fortin

et al. 2008

Journal of General Virology

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The poly(A)-binding protein (PABP) is an important translation initiation factor that binds to the polyadenylated 39 end of mRNA. We have previously shown that PABP2 interacts with the RNAdependent RNA polymerase (RdRp) and VPg-Pro of turnip mosaic virus (TuMV) within virusinduced vesicles. At least eight PABP isoforms are produced in Arabidopsis thaliana, three of which (PABP2, PABP4 and PABP8) are highly and broadly expressed and probably constitute the bulk of PABP required for cellular functions. Upon TuMV infection, an increase in protein and mRNA expression from PAB2, PAB4 and PAB8 genes was recorded. In vitro binding assays revealed that RdRp and the viral genome-linked protein (VPg-Pro) interact preferentially with PABP2 but are also capable of interaction with one or both of the other class II PABPs (i.e. PABP4 and PABP8). To assess whether PABP is required for potyvirus replication, A. thaliana single and double pab knockouts were isolated and inoculated with TuMV. All lines showed susceptibility to TuMV. However, when precise monitoring of viral RNA accumulation was performed, it was found to be reduced by 2.2-and 3.5-fold in pab2 pab4 and pab2 pab8 mutants, respectively, when compared with wild-type plants. PABP levels were most significantly reduced in the membrane-associated fraction in both of these mutants. TuMV mRNA levels thus correlated with cellular PABP concentrations in these A. thaliana knockout lines. These data provide further support for a role of PABP in potyvirus replication. INTRODUCTIONThe poly(A)-binding protein (PABP) is an abundant translation initiation factor in the cell that binds to the polyadenylated 39 end of mRNA. Its interaction with the eukaryotic translation initiation factor 4F complex (composed of the eIF4E, eIF4A and eIF4G proteins), which is bound to the 59 cap structure of the mRNA, results in the formation of a protein bridge that brings the 59 and 39 termini of the mRNA into proximity. This leads to a synergistic enhancement of translation (Sachs, 2000;Wells et al., 1998). PABP is also important for stability (BehmAnsmant et al., 2007;Parker & Song, 2004), biogenesis (Amrani et al., 1997;Brown & Sachs, 1998) and nuclear export of cellular mRNAs (Brune et al., 2005).Multiple PABP isoforms are normally found in animals and plants (Mangus et al., 2003). Eight PABP genes have been identified in Arabidopsis thaliana (Belostotsky, 2003).The corresponding proteins are divided into four classes based on gene expression and similarity. Class II genes (PAB2, PAB4 and PAB8) are highly and broadly expressed and probably encode the bulk of PABP required for cellular functions (Belostotsky, 2003).In animal cells, PABP is cleaved by proteinases of picornaviruses (Joachims et al., 1999;Kerekatte et al., 1999;Kuyumcu-Martinez et al., 2002, 2004bRodriguez Pulido et al., 2007;Zhang et al., 2007), caliciviruses (Kuyumcu-Martinez et al., 2004a) and retroviruses (Alvarez et al., 2006). The resultant cleavage leads to host translational shutdown (Joachims et al., 1999; KuyumcuMartinez ...

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“…The TEV 5ʹ-leader is sufficient to confer cap-independent translation, even in the absence of the 5ʹ-terminal VPg (a viral genomelinked protein) [58,59]. The TEV 5ʹ-leader contains an IRES element [58][59][60] that is notable for its small size, and represents one of the most compact viral elements capable of promoting cap-independent translation yet identified. A 5ʹ-proximal, 45-nt RNA pseudoknotencompassing domain (PK1) within the TEV 5ʹ-leader is essential to promote cap-independent translation [61].…”

Section: Interactions With Translation Initiation Factors (Eifs) and mentioning

confidence: 99%

Insights into the Molecular Antiviral Mechanism of Pokeweed Protein from Phytolacca americana

Aitbakieva¹,

Domashevskiy²

2016

Biochem Pharmacol (Los Angel)

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The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation

Cited by 106 publications

References 14 publications

Cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA.

Cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA.

Arabidopsis thaliana class II poly(A)-binding proteins are required for efficient multiplication of turnip mosaic virus

Insights into the Molecular Antiviral Mechanism of Pokeweed Protein from Phytolacca americana

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