The tobacco plasma membrane aquaporin NtAQP1

Siefritz, Franka; Biela, Alexander; Eckert, Martin; Otto, Beate; Uehlein, Norbert; Kaldenhoff, Ralf

doi:10.1093/jexbot/52.363.1953

Cited by 63 publications

(36 citation statements)

References 31 publications

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“…Aquaporins are known to significantly contribute to water movement in plants. Additionally, up-or down-regulation of aquaporin genes significantly induces cellular membrane water permeability (Siefritz et al, 2001;Aharon et al, 2003), and either induced or suppressed regulation has been observed during osmotic stress. Katsuhara et al (2003) observed that HvPIP2; 1 expression was decreased in the roots of barley under NaCl stress, while transcription levels of HvPIP1; 3 and HvPIP1; 5 remained unchanged.…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Zhao

Yan

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The effects of exogenous 5-aminolevulinic acid (ALA) on photosynthesis, plant growth, and the expression of two aquaporin genes in tomato seedlings under control and salinity conditions were investigated. Exogenous ALA application significantly improved net photosynthetic rate (P n ), total chlorophyll content, and plant biomass accumulation of tomato seedlings under salinity stress. As revealed by real-time PCR analyses, after treatment with ALA alone, expression of both LePIP1 and LePIP2 in the two tomato cultivars was upregulated at 2 h and subsequently decreased to normal levels. Under salinity stress, transcript levels of LePIP1 in both leaves and roots of salt-sensitive cultivars (cv. Zhongza No.9) increased significantly and were considerably higher than in cultivars exposed to ALA alone. In contrast, the expression levels of LePIP1 and LePIP2 in cvs. Jinpeng No.1 cultivars were slightly lower under salinity stress than under ALA treatment. In addition, transcript levels of both LePIP1 and LePIP2 in the roots of Jinpeng No. 1 cultivars were considerably lower than those Y.Y. Zhao et al. 6402©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6401-6412 (2015) in the roots of Zhongza No. 9 cultivars under salinity stress, regardless of ALA supplementation, implying that Jinpeng No. 1 cultivars had a better capacity to maintain membrane intrinsic protein stability. Further, ALA application distinctly counteracted the up-or down-regulation of LePIP1 and LePIP2 in both cultivars under salinity stress, in accordance with the improvements instomatal conductance, transpiration rate, and P n of tomato leaves. The results presented here indicate that ALA controls aquaporin expression, thus, presumably ALA regulates water homeostasis and enhances salt tolerance of tomato seedlings.

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Section: Discussionmentioning

confidence: 99%

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Zhao

Yan

et al. 2015

Genet. Mol. Res.

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“…Transgenic A. thaliana (Kaldenhoff et al, 1998) and Nicotiana tabacum (Siefritz et al, 2001) plants with down-regulation of gene pip1, showed reduced osmotic water permeability in the protoplasts of leaves and roots, respectively, compared to control plants (Martre et al, 2002). Besides, plants with A. thaliana pip1b antisense displayed dry root mass five times larger than did control plants (Kaldenhoff et al, 1998).…”

Section: Genesmentioning

confidence: 97%

Cloning and quantitative expression analysis of drought-induced genes in soybean

Stolf-Moreira¹,

Medri²,

Neumaier³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and droughtsensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. Sequences of Arabidopsis thaliana DREB-family proteins were used to build a phylogenetic tree through the alignment of the conserved regions near the AP2 domain. We found that Gmdreb1 is similar to Atrap2.1, which is located near the AtDREB1 and AtDREB2 families. The amplified fragment was cloned and sequenced; alignment with the sequence available at Genbank showed total similarity. Expression analysis showed that under drought: a) Gmdreb1 expression increased in leaves and roots of both genotypes and expression level changes occurred that were correlated with the length of the water-deficit period; b) there 859 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (2): 858-867 (2010) Cloning and quantitative expression analysis in soybean were increased expression levels of Gmdefensin in roots of MG/BR46; c) expression of Gmgols increased in leaves and roots of the two genotypes; d) Gmpip1b expression generally increased, except in roots of BR16, and e) the same was found for Gmereb, except in roots of MG/BR46.

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“…Based on its high abundance in the roots, especially around the xylem vessels (Otto and Kaldenhoff, 2000;Siefritz et al, 2001), its impact on increasing the water permeability of Xenopus oocytes (Biela et al, 1999), and the decrease in root Lp of NtAQP1-silenced plants (Siefritz et al, 2002), NtAQP1 was suggested to play a role in controlling root L p . This activity might be related to abiotic stress conditions, since NtAQP1 is a stress-induced gene, it is regulated by an abscisic acid (ABA)-sensitive promoter (Siefritz et al, 2001), and it shows a significant increase in root transcript level during drought stress (Mahdieh et al, 2008). Moreover, NtAQP1-silenced plants reveal higher drought sensitivity than control plants (Siefritz et al, 2002).…”

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confidence: 99%

The Role of Tobacco Aquaporin1 in Improving Water Use Efficiency, Hydraulic Conductivity, and Yield Production Under Salt Stress

et al. 2009

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Tobacco (Nicotiana tabacum; C3) plants increase their water use efficiency (WUE) under abiotic stress and are suggested to show characteristics of C4 photosynthesis in stems, petioles, and transmitting tract cells. The tobacco stress-induced Aquaporin1 (NtAQP1) functions as both water and CO 2 channel. In tobacco plants, overexpression of NtAQP1 increases leaf net photosynthesis (A N ), mesophyll CO 2 conductance, and stomatal conductance, whereas its silencing reduces root hydraulic conductivity (L p ). Nevertheless, interaction between NtAQP1 leaf and root activities and its impact on plant WUE and productivity under normal and stress conditions have never been suggested. Thus, the aim of this study was to suggest a role for NtAQP1 in plant WUE, stress resistance, and productivity. Expressing NtAQP1 in tomato (Solanum lycopersicum) plants (TOM-NtAQP1) resulted in higher stomatal conductance, whole-plant transpiration, and A N under all conditions tested. In contrast to controls, where, under salt stress, L p decreased more than 3-fold, TOM-NtAQP1 plants, similar to maize (Zea mays; C4) plants, did not reduce L p dramatically (only by approximately 40%). Reciprocal grafting provided novel evidence for NtAQP1's role in preventing hydraulic failure and maintaining the whole-plant transpiration rate. Our results revealed independent, albeit closely related, NtAQP1 activities in roots and leaves. This dual activity, which increases the plant's water use and A N under optimal and stress conditions, resulted in improved WUE. Consequently, it contributed to the plant's stress resistance in terms of yield production under all tested conditions, as demonstrated in both tomato and Arabidopsis (Arabidopsis thaliana) plants constitutively expressing NtAQP1. The putative involvement of NtAQP1 in tobacco's C4-like photosynthesis characteristics is discussed.

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The tobacco plasma membrane aquaporin NtAQP1

Cited by 63 publications

References 31 publications

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Effects of exogenous 5-aminolevulinic acid on photosynthesis, stomatal conductance, transpiration rate, and PIP gene expression of tomato seedlings subject to salinity stress

Cloning and quantitative expression analysis of drought-induced genes in soybean

The Role of Tobacco Aquaporin1 in Improving Water Use Efficiency, Hydraulic Conductivity, and Yield Production Under Salt Stress

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