The tonoplast intrinsic aquaporin (TIP) subfamily of Eucalyptus grandis: Characterization of EgTIP2, a root-specific and osmotic stress-responsive gene

Rodrigues, Marcela Iara; Bravo, Juliana Pereira; Sassaki, Flávio Tetsuo; Severino, Fabio; Maia, Ivan de Godoy

doi:10.1016/j.plantsci.2013.09.005

Cited by 32 publications

(41 citation statements)

References 40 publications

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“…Freshly harvested vegetative and reproductive organs/tissues (1 month-old plantlets; pool of leaves of different ages; roots from 1 and 6 month-old plantlets; stems from 1 and 6 month-old plantlets; vascular cambium samples from 6 year-old trees; flower; flower buds; and fruits) were obtained from Eucalyptus grandis essentially as described previously (Rodrigues et al, 2013). After harvesting, fresh samples were frozen immediately in liquid nitrogen until total RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA extraction and cDNA synthesis were performed as previously described (Rodrigues et al, 2013). The qPCR analyses were carried out using Power SYBR Green Master Mix (Applied Biosystems) and a StepOnePlus Real Time PCR System (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…Reports on the expression of a set of TIP genes revealed differential regulation in response to environmental constraints, especially drought and salinity, and to abscisic acid (ABA) (Alexandersson et al, 2005; Boursiac et al, 2005; Rodrigues et al, 2013; Regon et al, 2014). Supporting the importance of these proteins in plant stress adaptation, TIPs were shown to improve drought and salt tolerance when overexpressed in transgenic plants (Peng et al, 2007; Khan et al, 2015; Li and Cai, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In forest and fruit tree species, genome-wide surveys revealed the presence of 17 TIP genes in Populus trichocarpa (Gupta and Sankararamakrishnan, 2009) and Hevea brasiliensis (Zou et al, 2015a), while 10 and 11 genes were identified in Vitis vinifera and Citrus sinensis , respectively (Shelden et al, 2009; Martins et al, 2015). Intriguingly, a preliminary study identified a relatively small number of TIP genes (five in total) in the Eucalyptus genome (Rodrigues et al, 2013). Taking into consideration the evolutionary history of the eucalyptus genome (Myburg et al, 2014), such a comparatively low number of genes is unexpected and requires additional investigation.…”

Section: Introductionmentioning

confidence: 99%

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The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

et al. 2016

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Plant aquaporins are water channels implicated in various physiological processes, including growth, development and adaptation to stress. In this study, the Tonoplast Intrinsic Protein (TIP) gene subfamily of Eucalyptus, an economically important woody species, was investigated and characterized. A genome-wide survey of the Eucalyptus grandis genome revealed the presence of eleven putative TIP genes (referred as EgTIP), which were individually assigned by phylogeny to each of the classical TIP1–5 groups. Homology modeling confirmed the presence of the two highly conserved NPA (Asn-Pro-Ala) motifs in the identified EgTIPs. Residue variations in the corresponding selectivity filters, that might reflect differences in EgTIP substrate specificity, were observed. All EgTIP genes, except EgTIP5.1, were transcribed and the majority of them showed organ/tissue-enriched expression. Inspection of the EgTIP promoters revealed the presence of common cis-regulatory elements implicated in abiotic stress and hormone responses pointing to an involvement of the identified genes in abiotic stress responses. In line with these observations, additional gene expression profiling demonstrated increased expression under polyethylene glycol-imposed osmotic stress. Overall, the results obtained suggest that these novel EgTIPs might be functionally implicated in eucalyptus adaptation to stress.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

et al. 2016

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“…In Picea glauca, TIP2:1 is detected in roots, stems, needles, reproductive parts, while TIP2:2 is detected only in stems (Laur, Hacke 2014). TIP overexpression in transgenic plants has been associated with increased plant growth rate and biomass production (Rodrigues et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Expression of the Scots pine (Pinus sylvestris) TIP-like and sucrose synthase genes during two growing seasons and correlation with relative wood densities

2017

EEB

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Scots pine is one of the dominant tree species in Latvian forests and has great ecological, social and economic importance globally. Research on connecting molecular data with phenotype is still developing for non-model plant species. To study the linkages between gene expression and desirable wood properties in Scots pine and to determine the dynamics of gene expression for organisms growing in field conditions, we investigated the expression of two genes, previously reported to be involved in wood formation processes. Fifty Scots pine individuals with differing relative wood densities were analysed at one time point during early and late wood formation during the first growing season. During the second growing season, a subset of fifteen trees with large gene expression differences detected during the first season were analysed at four time points during early wood formation and five time points during late wood formation. We found that during both growing seasons, sucrose synthase gene expression tended to be higher for trees with higher wood density, while the tonoplast intrinsic protein-like gene was more highly expressed in trees with lower wood density. Both genes showed differential gene expression dynamics during the second growth season, but at some time points there was correlation with gene expression in the first season.

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Mitochondrial Targeting of Catalytic RNAs

Mileshina

Niazi

Wyszko

et al. 2015

Methods in Molecular Biology

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Genetic transformation of mitochondria in multicellular eukaryotes has remained inaccessible, hindering fundamental investigations and applications to gene therapy or biotechnology. In this context, we have developed a strategy to target nuclear transgene-encoded RNAs into mitochondria in plants. We describe here mitochondrial targeting of trans-cleaving ribozymes destined to knockdown organelle RNAs for regulation studies and inverse genetics and biotechnological purposes. The design and functional assessment of chimeric RNAs combining the ribozyme and the mitochondrial shuttle are detailed, followed by all procedures to prepare constructs for in vivo expression, generate stable plant transformants, and establish target RNA knockdown in mitochondria.

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The tonoplast intrinsic aquaporin (TIP) subfamily of Eucalyptus grandis: Characterization of EgTIP2, a root-specific and osmotic stress-responsive gene

Cited by 32 publications

References 40 publications

The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles

Expression of the Scots pine (Pinus sylvestris) TIP-like and sucrose synthase genes during two growing seasons and correlation with relative wood densities

Mitochondrial Targeting of Catalytic RNAs

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