The Tonoplast Intrinsic Protein Gene <i>KvTIP3</i> is Responsive to Different Abiotic Stresses in <i>Kosteletzkya virginica</i>

Liu, Xiaohua; Cheng, Jieshan; Jiang, Fudong; Liang, Mao; Han, Juan; Zhang, Juan; Zhang, Hongxia; Tang, Xiaoli

doi:10.1155/2020/2895795

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…Downregulation of several TIPs in P. nuttalliana by the NaCl treatment may also have important consequences to water homeostasis by regulating water fluxes across the tonoplast. Several studies revealed that TIP aquaporins, which are primarily located in the vacuolar membrane (Maurel et al, 2008), play important roles under salt stress (Afzal et al, 2016;Liu X. et al, 2020). In addition to the intracellular water movement, TIPs are involved in transporting small solutes and gases (Nozaki et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

2021

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Elevated soil salinity exacerbated by human activities and global climate change poses serious threats to plant survival. Although halophytes provide many important clues concerning salt tolerance in plants, some unanswered questions remain to be addressed, including the processes of water and solute transport regulation. We performed high-throughput RNA-sequencing in roots and metabolome characterizations in roots and leaves of Puccinellia nuttalliana halophytic grass subjected to 0 (control) and 150 mM NaCl. In RNAseq, a total of 31 Gb clean bases generated were de novo assembled into 941,894 transcripts. The PIP2;2 and HKT1;5 transcript levels increased in response to the NaCl treatment implying their roles in water and ion homeostasis. Several transcription factors, including WRKY39, DEK3, HY5, and ABF2, were also overexpressed in response to NaCl. The metabolomic analysis revealed that proline and dopamine significantly increased due to the upregulation of the pathway genes under salt stress, likely contributing to salt tolerance mechanisms. Several phosphatidylcholines significantly increased in roots suggesting that the alterations of membrane lipid composition may be an important strategy in P. nuttalliana for maintaining cellular homeostasis and membrane integrity under salt stress. In leaves, the TCA cycle was enriched suggesting enhanced energy metabolism to cope with salt stress. Other features contributing to the ability of P. nuttalliana to survive under high salinity conditions include salt secretion by the salt glands and enhanced cell wall lignification of the root cells. While most of the reported transcriptomic, metabolomics, and structural alterations may have consequences to water balance maintenance by plants under salinity stress, the key processes that need to be further addressed include the role of the changes in the aquaporin gene expression profiles in the earlier reported enhancement of the aquaporin-mediated root water transport.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

2021

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“…Kosteletzkya pentacarpos L. (seashore mallow), formerly known as Kosteletzkya virginica (Liu et al, 2020(Liu et al, , 2021, is a perennial halophyte belonging to the Malva genus of the Malvaceae family. It is naturally distributed on the salt marshy coasts of eastern United States, and is commercially used for the production of oil (Ruan et al, 2008), feed (Sun et al, 2019), medicines (Bai et al, 2015), and beauty products (Qin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The heat shock protein gene KvHSP70 is sensitive to NaCl stress and significantly improves the salinity tolerance of transgenic tobacco plants (Tang et al, 2020). Subsequently, the salinity stress-sensitive genes cloned from K. pentacarpos, such as the chloroplast small heat shock protein gene KvHSP26 and the tonoplast intrinsic protein gene KvTIP3, are potential candidates for molecular plant breeding (Liu et al, 2020(Liu et al, , 2021.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analyses of Transcriptome and Chlorophyll Fluorescence Characteristics Reveal the Mechanism Underlying Saline–Alkali Stress Tolerance in Kosteletzkya pentacarpos

Zhou

Wang

et al. 2022

Front. Plant Sci.

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In recent years, soil salinization has become increasingly severe, and the ecological functions of saline–alkali soils have deteriorated because of the lack of plants. Therefore, understanding the tolerance mechanisms of saline–alkali-tolerant plants has become crucial to restore the ecological functions of saline–alkali soils. In this study, we evaluated the molecular mechanism underlying the tolerance of Kosteletzkya pentacarpos L. (seashore mallow) seedlings treated with 0.05 or 0.5% saline–alkali solution (NaCl: NaHCO3 = 4:1 mass ratio) for 1 and 7 days. We identified the key genes involved in tolerance to saline–alkali stress using orthogonal partial least squares regression analysis (OPLS-RA) based on both chlorophyll fluorescence indexes and stress-responsive genes using transcriptome analysis, and, finally, validated their expression using qRT-PCR. We observed minor changes in the maximum photochemical efficiency of the stressed seedlings, whose photosynthetic performance remained stable. Moreover, compared to the control, other indicators varied more evidently on day 7 of 0.5% saline–alkali treatment, but no variations were observed in other treatments. Transcriptome analysis revealed a total of 54,601 full-length sequences, with predominantly downregulated differentially expressed gene (DEG) expression. In the high concentration treatment, the expression of 89.11 and 88.38% of DEGs was downregulated on days 1 and 7, respectively. Furthermore, nine key genes, including KpAGO4, KpLARP1C, and KpPUB33, were involved in negative regulatory pathways, such as siRNA-mediated DNA methylation, inhibition of 5′-terminal oligopyrimidine mRNA translation, ubiquitin/proteasome degradation, and other pathways, including programmed cell death. Finally, quantitative analysis suggested that the expression of key genes was essentially downregulated. Thus, these genes can be used in plant molecular breeding in the future to generate efficient saline–alkali–tolerant plant germplasm resources to improve the ecological functions of saline–alkali landscapes.

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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica

Liu

Lizi

et al. 2021

International Journal of Genomics

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Small heat shock proteins (sHSPs) are a group of chaperone proteins existed in all organisms. The functions of sHSPs in heat and abiotic stress responses in many glycophyte plants have been studied. However, their possible roles in halophyte plants are still largely known. In this work, a putative sHSP gene KvHSP26 was cloned from K. virginica. Bioinformatics analyses revealed that KvHSP26 encoded a chloroplastic protein with the typical features of sHSPs. Amino acid sequence alignment and phylogenetic analysis demonstrated that KvHSP26 shared 30%-77% homology with other sHSPs from Arabidopsis, cotton, durian, salvia, and soybean. Quantitative real-time PCR (qPCR) assays exhibited that KvHSP26 was constitutively expressed in different tissues such as leaves, stems, and roots, with a relatively higher expression in leaves. Furthermore, expression of KvHSP26 was strongly induced by salt, heat, osmotic stress, and ABA in K. virginica. All these results suggest that KvHSP26 encodes a new sHSP, which is involved in multiple abiotic stress responses in K. virginica, and it has a great potential to be used as a candidate gene for the breeding of plants with improved tolerances to various abiotic stresses.

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The Tonoplast Intrinsic Protein Gene KvTIP3 is Responsive to Different Abiotic Stresses in Kosteletzkya virginica

Cited by 4 publications

References 51 publications

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

Integrated Analyses of Transcriptome and Chlorophyll Fluorescence Characteristics Reveal the Mechanism Underlying Saline–Alkali Stress Tolerance in Kosteletzkya pentacarpos

The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica

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