The topical application of low-temperature argon plasma enhances the anti-inflammatory effect of Jaun-ointment on DNCB-induced NC/Nga mice

Abstract

BackgroundJaun-ointment (JO), also known as Shiunko in Japan, is one of the most popular medicinal formulae used in Korean traditional medicine for the external treatment of skin wound and inflammatory skin conditions. Since JO is composed of crude mixture of two herbal extracts (radix of Lithospermum erythrorhizon Siebold & Zucc and Angelica gigas Nakai), those been proved its anti-inflammatory activities in-vitro and in-vivo, JO has been expected as a good alternative treatment option for atopic dermatitis (AD). However, due to the lack of strategies for the penetrating methods of JO’s various anti-inflammatory elements into the skin, an effective and safe transdermal drug delivery system needs to be determined. Here, low-temperature argon plasma (LTAP) was adopted as an ancillary partner of topically applied JO in a mice model of AD and the effectiveness was examined.MethodsDorsal skins of NC/Nga mice were challenged with DNCB (2,4-dinitrochlorobenzene) to induce AD. AD-like skin lesions were treated with JO alone, or in combination with LTAP. Inflammatory activity in the skin tissues was evaluated by histological analysis and several molecular biological tests.ResultsLTAP enhanced the effect of JO on AD-like skin lesion. Topical application of JO partially inhibited the development of DNCB-induced AD, shown by the moderate reduction of eosinophil homing and pro-inflammatory cytokine level. Combined treatment of JO and LTAP dramatically inhibited AD phenotypes. Interestingly, treatment with JO alone did not affect the activity of nuclear factor (NF)κB/RelA in the skin, but combined treatment of LTAP-JO blocked DCNB-mediated NFκB/RelA activation.ConclusionsLTAP markedly enhanced the anti-inflammatory activity of JO on AD-like skin lesions. The effect of LTAP may be attributed to enhancement of drug penetration and regulation of NFκB activity. Therefore, the combination treatment of JO and LTAP could be a potential strategy for the treatment of AD.

Bleomycin Aggravates Atopic Dermatitis via Lung Inflammation in 2,4-Dinitrochlorobenzene-Induced NC/Nga Mice

“…AD-like symptoms were evaluated by dermatitis skin score (Method 2.6) and the skin clinical score significantly increased in both DNCB-Control and DNCB-BLM mice when compared to NC/Nga-Normal mice ( F -value = 19.696 and p ≤ 0.01) ( Figure 2A ). Consistent with previous reports ( Choi et al, 2017 ), the overall clinical symptoms of AD, including erythema, hemorrhage, edema, scarring, excoriation, and lichenification, were induced by the repeated application of DNCB to the back skin of the NC/Nga mice for 2 weeks ( p = 0.003). More importantly, BLM administered to the lung dramatically increased the skin clinical severity score of AD in DNCB-treated mice ( p ≤ 0.001, compared to NC/Nga-Normal mice; p = 0.049, compared to DNCB-Control mice) ( Figure 2A ).…”

Isoprenylcysteine Carboxyl Methyltransferase and Its Substrate Ras Are Critical Players Regulating TLR-Mediated Inflammatory Responses

“…An AD mouse model was created by administering DNCB to NC/Nga mice (Daehan Biolink, Osong, Korea), as previously described [39]. Briefly, 1% DNCB (200 µL) in acetone/olive oil (3:1) was applied for sensitization to DNCB.…”

Tabetri™ (Tabebuia avellanedaeEthanol Extract) Ameliorates Atopic Dermatitis Symptoms in Mice

“…An AD mouse model was generated by treatment of the NC/Nga mice with DNCB, as previously described with a slight modification [ 41 ]. Briefly, 0.2 ml of 1% DNCB in acetone/olive oil (3 : 1) was applied for DNCB sensitization.…”