The total alkaloid fraction of bulbs of Fritillaria cirrhosa displays anti-inflammatory activity and attenuates acute lung injury

Wang, Dongdong; Yang, Jie; Du, Qingdan; Li, Houcong; Wang, Shu

doi:10.1016/j.jep.2016.08.009

Cited by 53 publications

(27 citation statements)

References 40 publications

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“…In this study, we performed RNA-Seq to generate and compare the transcriptomes of the wild and in vitro-regenerated F. cirrhosa bulbs. We comprehensively analyzed the gene expression profiles of F. cirrhosa with emphasis on genes related to the biosynthesis of steroidal alkaloid biosynthetic pathways, which are the major bioactive ingredients responsible for the pharmacological features (Jian et al 2012;Wang et al 2016). Our results demonstrated that steroidal alkaloid biosynthesis-related genes were universally upregulated in the in vitro-generated bulbs.…”

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confidence: 90%

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Zhao

Zhang

et al. 2018

3 Biotech

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The bulbs of is wildly used in traditional Chinese medicine to treat lung-related disease, which has recently been found to have antitussive, anti-inflammatory, antihypertensive and anti-tumor activity. Steroidal alkaloids are the major effective ingredients of. In the current study, we demonstrated an efficient strategy for bulb regeneration in vitro by cytokinin/auxin induction. Our data showed that the regenerated bulbs accumulated higher alkaloid content that the wild ones. We further performed RNA-seq and bioinformatics analysis to study the gene expression profile, especially those related to alkaloids biosynthesis. KEGG pathway annotation identified genes related to "Metabolic pathways" were the most abundant (2644, 26.0%), followed by those for "Biosynthesis of secondary metabolites" (1319, 13.0%) among the 113,865 unigenes identified. Further analysis suggested MEP pathway, other than MVA pathway, might be the major route for steroidal alkaloid biosynthesis of, as all the key genes in this pathway were found to be unregulated in our study. We also showed that accumulation of different phytochemicals was linked to plant hormone addition. Our current study demonstrated that in vitro cultivation is a promising strategy for mass production of steroidal alkaloids for pharmacological industry.

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confidence: 90%

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Zhao

Zhang

et al. 2018

3 Biotech

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“…Fritillaria represents the bulbs of various plants from the Fritillaria genus in the Liliaceae family, which have been used in traditional Chinese medicine for a long period of time because of their effects of clearing heat, moistening the lung, resolving phlegm, and relieving coughs, for the treatment of a cough caused by lung heat and dryness, a low sputum dry cough, a cough due to a yin deficiency, and sputum with blood [ 1 , 2 , 3 ]. Various bioactive chemical components have been found in Fritillaria , mainly consisting of alkaloids, saponins, terpenes, and glycosides.…”

Section: Introductionmentioning

confidence: 99%

“…Various bioactive chemical components have been found in Fritillaria , mainly consisting of alkaloids, saponins, terpenes, and glycosides. Studies on the chemical constituents and pharmacological actions have shown that the active ingredients resulting in the cough-curing and phlegm-reducing effects were alkaloids [ 3 , 4 ]. Normally, there are five kinds of Fritillaria , including Fritillaria Cirrhosa, Fritillaria ussuriensis, Fritillaria pallidiflora, Fritillaria thunbergii, and Fritillaria hupehensis.…”

Section: Introductionmentioning

confidence: 99%

Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

2017

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The Fritillaria genus, including different kinds of medicinal and edible plants belonging to the Liliaceae family which have the function of treating and relieving a cough and eliminating phlegm, is widely planted in Xinjiang (China). There are few comprehensive studies reporting on the characterization of the chemical constituents of Fritillaria from Xinjiang, and to date, no work describing the quantitative differences between the components in Fritillaria from Xinjiang and related species. The purpose of this study was to develop qualitative and quantitative analytical methods by Ultra Performance Liquid Chromatography-Quadrupole Time-of-flight Mass Spectrometry (UPLC-QTOF-MS) for the rapid quantification and quantitation of alkaloids in wild and cultivated Xinjiang Fritillaria, which could be used in the quality control of medicine based on this natural herb. Using the UPLC-QTOF-MS method, the chemical constituents of Xinjiang Fritillaria were identified by fragmentation information and retention behavior, and were compared to reference standards. Furthermore, a quantitative comparision of four major alkaloids in wild and cultivated Xinjiang Fritillaria was conducted by determining the content of Sipeimine-3β-d-glucoside, Sipeimine, Peimisine, and Yibeinoside A, respectively. A total of 89 characteristic peaks, including more than 40 alkaloids, were identified in the chromatographic results of Fritillaria. Four main alkaloids were quantified by using a validated method based on UPLC-QTOF-MS. The relative contents of Sipeimine-3β-d-glucoside, Sipeimine, Peimisine, and Yibeinoside A varied from 0.0013%~0.1357%, 0.0066%~0.1218%, 0.0033%~0.0437%, and 0.0019%~0.1398%, respectively. A rough separation of wild and cultivated Fritillaria could be achieved by the cluster analysis method.

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“…To date, a variety of isosteroidal alkaloids, mainly including imperialine, peimisine, peimine, and peiminine, have been isolated and described from Fritillaria species [6][7][8][9]. Previous investigations on the pharmacology and phytochemistry suggested that the steroidal alkaloids, especially isosteroidal alkaloids, were responsible for the antitussive, expectorant, anti-inflammatory and antinociceptive effects of these herbs [10][11][12][13][14]. F. taipaiensis is no different from F. cirrhosa as a commodity due to their similar chemical constituents [15,16].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis provides insights into key gene(s) involved in steroidal alkaloid biosynthesis in the medicinally important herb Fritillaria taipaiensis

Wang¹,

Luo²,

Wei³

et al. 2019

Preprint

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Background Fritillariae Cirrhosae Bulbus, which is a traditional antitussive and expectorant medicine in China, is derived from six original plants. Among these six species, all except for Fritillaria taipaiensis and Fritillaria unibracteata var. wabuensis have been recorded in the National Protected Plants III. Their major bioactive compounds, including imperialine and peimisine, are steroidal alkaloids, derived from the steroid synthesis pathway. However, research on the F. taipaiensis genome, transcriptomes and steroid-synthesis-related genes has been lacking for a long time. In this study, the first transcriptome sequencing of F. taipaiensis was performed. Results A total of 94,396,694 (~11.4 Gb) high-quality reads obtained using paired-end Illumina sequencing were de novo assembled into 190,350 transcripts. Functional annotation with multiple public databases identified an array of genes involved in steroidal alkaloid biosynthesis and other secondary metabolite pathways, including steroid and terpenoid backbone biosynthesis, and important oxidoreductases. The large number of differentially expressed transcripts together with CYPs suggests the involvement of these candidates in tissue-specific expression. The expression analysis revealed that bulbs may be the main site of the upstream steroidal alkaloid biosynthesis pathway, and we speculated that the downstream reactions, including the oxidation-reduction reaction catalyzed by CYPs, might also occur primarily in bulbs. Conclusion In conclusion, the comprehensive transcriptome dataset created in this study will serve as a resource for the identification of potential candidates for the genetic manipulation of targeted bioactive metabolites and will also contribute to the development of functionally relevant molecular marker resources to expedite molecular breeding and conservation efforts for F. taipaiensis.

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The total alkaloid fraction of bulbs of Fritillaria cirrhosa displays anti-inflammatory activity and attenuates acute lung injury

Cited by 53 publications

References 40 publications

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

Transcriptome analysis provides insights into key gene(s) involved in steroidal alkaloid biosynthesis in the medicinally important herb Fritillaria taipaiensis

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