The Toxic and Stress Responses of Cultured Human Retinal Pigment Epithelium (ARPE19) and Human Glial Cells (SVG) in the Presence of Triamcinolone

Yeung, Chi Kong; Chan, Kwok Ping; Chiang, Sylvia W. Y.; Pang, Chi Pui; Lam, Dennis S C

doi:10.1167/iovs.03-0490

Cited by 99 publications

(70 citation statements)

References 26 publications

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“…We therefore speculate that, in the present study, the intraocular use of TA decreased postoperative inflammation, and inhibited subsequent fibrous tissue formation and the recurrence of RD to some extent. This possibility is supported by clinical observations (13,16,25), and by the finding that TA inhibits RPE and retinal glial cell proliferation in vitro (26). Corticosteroids are known to elevate IOP in susceptible patients, much like the elevation of IOP observed in some eyes following TA injection (27)(28)(29)(30).…”

Section: Discussionmentioning

confidence: 81%

Long-Term Clinical Outcomes and Therapeutic Benefits of Triamcinolone-Assisted Pars Plana Vitrectomy for Proliferative Vitreoretinopathy: A Case Study

Ueno

Enaida

Hata

et al. 2007

European Journal of Ophthalmology

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Purpose. To investigate intraoperative visibility and long-term clinical outcome following triamcinolone acetonide (TA)-assisted pars plana vitrectomy (PPV) for proliferative vitreoretinopathy (PVR). Methods. A retrospective interventional noncomparative clinical study was carried out on 21 eyes from 21 patients with more than grade C2 PVR, all of whom underwent TA-assisted PPV. Two of the specimens were observed with an electron microscope. After treatment, outcome measures, including changes in best-corrected visual acuity (BCVA), intraocular pressure (IOP) elevation, corneal pathology, and occurrence of endophthalmitis, were recorded. Patient follow-up time was >36 months (mean ± standard deviation = 47.3 ± 6.7 months). Results. TA improved the intraoperative visualization of the epiretinal membrane (ERM), allowing it to be easily removed together with the partially internal limiting membrane (ILM) using microforceps. The excised tissue consisted of proliferative cells and an extracellular matrix underlying the ILM. After the operation, 71.4% of the eyes had improved BCVA. Three of the eyes showed sustained IOP elevation (14.3%); two of these cases were controlled by the administration of eyedrops, while the third required filtering surgery. In two cases, an absorption delay of the TA granule on the retinal surface was observed. One eye developed corneal stromal opacity. No other severe complications occurred during the observation period. Conclusions. TA-assisted PPV offers improved visualization during the surgical management of PVR, and allows surgeons to excise the ERM safely and effectively without the risk of serious complications.

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Section: Discussionmentioning

confidence: 81%

Long-Term Clinical Outcomes and Therapeutic Benefits of Triamcinolone-Assisted Pars Plana Vitrectomy for Proliferative Vitreoretinopathy: A Case Study

Ueno

Enaida

Hata

et al. 2007

European Journal of Ophthalmology

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“…Our recent in vitro studies showed that triamcinolone could exert a cytotoxic effect on retinal pigment epithelial cells and glial cells. 20,21 In addition to localizing in the vitreous cavity, triamcinolone has been detected in the aqueous humor after intravitreal injections. 22 The presence of triamcinolone acetonide in the anterior chamber may affect the health of the corneal endothelium, which plays an important role in the function of the cornea.…”

Section: Introductionmentioning

confidence: 99%

Ocular-hypertensive response and corneal endothelial changes after intravitreal triamcinolone injections in Chinese subjects: a 6-month follow-up study

Chan

Fan

Chan

et al. 2004

Eye

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Purpose To assess the intraocular pressure (IOP) and corneal endothelial changes, over a 6-month period, after a single injection of intravitreal triamcinolone (ivTA) in Chinese patients. Methods A total of 43 eyes of 43 consecutive Chinese patients with various macular diseases received a single bolus injection of 4 mg ivTA, of which, 14 eyes with significant cataracts underwent simultaneous phacoemulsification and primary intraocular lens implantation. IOP was measured preoperatively and weekly in the first month, and then monthly until 6 months postinjection. Specular microscopy was performed on 24 of the 29 eyes without simultaneous cataract surgery, preoperatively and at months 1, 3, and 6. Results All patients completed 6 months of follow-up. Nine out of 43 (20.9%) eyes had IOP 421 mmHg. Their mean maximum IOP was 29.2 mmHg (range 23.0-37.0), necessitating the use of 2.0 types of topical antiglaucomatous medications on average. The IOP elevation occurred at a mean of 5.2 weeks (range 1-17) postinjection. All IOPs returned to normal, without additional antiglaucomatous medications, by 6 months. There was no statistically significant difference (paired t-test, Po0.05) in the corneal endothelial cell count and other specular microscopy parameters up to 6 months after the injections. Conclusion A single 4 mg bolus injection of ivTA appeared to have no harmful effects on the corneal endothelium. IvTA caused transient IOP elevations in 20.9% of Chinese patients, similar to that observed in Caucasians. As the IOP rise can occur as early as 1 week after the injection, early monitoring will help its early detection and prevent optic nerve damage.

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“…Similar improvement in drugdelivery efficiency in response to polymeric nanoparticles has also been reported by previous researchers. 42 The most common injection doses of triamcinolone acetonide used in ophthalmic practice are 4-20 mg. 10 Because the vitreous volume in humans is about 4 mL, 32 the equivalent vitreous concentration of this corticosteroid thus corresponds to 1-5 mg/mL. Although the experimental TAA concentration in the present TAA/DA-Chit nanoparticles (0.1 mg/mL) was much less than the clinical concentration of triamcinolone acetonide, downregulation of VEGF mRNA expression was significant.…”

Section: Vegf Mrna Expression Efficiencymentioning

confidence: 79%

“…13,14 For example, triamcinolone acetonide is toxic to human retinal pigment epithelial cells in concentrations from 0.01 mg/mL to 1 mg/mL, resulting in apoptosis. 31,32 The cytotoxicity of TAA/DA-Chit nanoparticles was thus evaluated using the CCK-8 assay. 26 After human retinal pigment epithelial cells had been incubated with TAA at 0.1 mg/mL for 1-5 days, cell viability decreased from 78.3% to 40.4% (Figure 6), suggesting strong cytotoxicity.…”

Section: Cytotoxicity Of Taa/da-chit Nanoparticlesmentioning

confidence: 99%

Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells

Zhou¹,

Yang²,

Li³

et al. 2012

IJN

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Background:The purpose of this study was to investigate the downregulation of mRNA expression of vascular endothelial growth factor (VEGF) by triamcinolone acetonide acetate (TAA)-loaded chitosan nanoparticles in human retinal pigment epithelial cells. Methods: TAA-loaded deoxycholic acid-modified chitosan (TAA/DA-Chit) nanoparticles were prepared via a self-assembly mechanism, and their morphology and zeta potential were examined by transmission electron microscopy and zeta potential analysis, respectively. DA-Chit and TAA/DA-Chit nanoparticle toxicity was evaluated using a Cell Counting Kit-8 assay. The efficiency of cellular uptake was determined using fluorescein isothiocyanate-labeled DA-Chit nanoparticles, in place of TAA/DA-Chit nanoparticles, assessed by both inverted fluorescence microscopy and flow cytometry. Downregulation of VEGF mRNA expression by TAA/DAChit nanoparticles was further investigated by real-time reverse transcription polymerase chain reaction (RT-PCR) assay of the treated human retinal pigment epithelial cells. Results: TAA/DA-Chit nanoparticles were prepared with a TAA-loading capacity in the range of 12%-82%, which increased the water solubility of TAA from 0.3 mg/mL to 2.1 mg/mL. These nanoparticles showed oblate shapes 100-550 nm in size in transmission electron microscopic images and had positive zeta potentials. The Cell Counting Kit-8 assay indicated that the DAChit and TAA/DA-Chit nanoparticles had no toxicity and low toxicity, respectively, to human retinal pigment epithelial cells. Fluorescein isothiocyanate-labeled DA-Chit nanoparticle uptake by human retinal pigment epithelial cells was confirmed by inverted fluorescence microscopy and flow cytometry. Real-time RT-PCR assay showed that the VEGF mRNA level decreased after incubation of human retinal pigment epithelial cells with TAA/DA-Chit nanoparticles. Conclusion: TAA/DA-Chit nanoparticles had a downregulating effect on VEGF mRNA expression in human retinal pigment epithelial cells and low cytotoxicity, which might be beneficial characteristics for the development of future treatment for diabetic retinopathy.

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The Toxic and Stress Responses of Cultured Human Retinal Pigment Epithelium (ARPE19) and Human Glial Cells (SVG) in the Presence of Triamcinolone

Cited by 99 publications

References 26 publications

Long-Term Clinical Outcomes and Therapeutic Benefits of Triamcinolone-Assisted Pars Plana Vitrectomy for Proliferative Vitreoretinopathy: A Case Study

Long-Term Clinical Outcomes and Therapeutic Benefits of Triamcinolone-Assisted Pars Plana Vitrectomy for Proliferative Vitreoretinopathy: A Case Study

Ocular-hypertensive response and corneal endothelial changes after intravitreal triamcinolone injections in Chinese subjects: a 6-month follow-up study

Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells

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