The toxic effects of in situ exposure of a native fish species (Anabas testudineus) to electronic waste pollution

Zhang, Wanglong; Xie, Heidi Qunhui; Zou, Xianghui; Li, Jiao; Xu, Li; Li, Yunping; Zhou, Zhiguang; Jin, Tao; Ma, Dan; Zhao, Bin

doi:10.1016/j.scitotenv.2019.06.479

Cited by 23 publications

(8 citation statements)

References 33 publications

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“…Previous studies have reported the resistance of the climbing perch ( Anabas testudineus , a freshwater teleost) to environmental stressors including hypoxia, high salinity, low pH, and environmental ammonia. − Our recent field investigations in electronic-waste dismantling regions provide further support for the idea that A. testudineus is adapted to survive in highly contaminated water bodies . Heavy metals and some persistent organic pollutants (POPs) were identified as major contaminants that are present there at extremely high concentrations. , …”

Section: Introductionmentioning

confidence: 69%

Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a

Zhang

Xie

et al. 2019

Environ. Sci. Technol.

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Field investigations have revealed the ability of the climbing perch Anabas testudineus to survive in highly contaminated water bodies. The aryl hydrocarbon receptor (AhR) pathway is vital in mediating the toxicity of aromatic hydrocarbon contaminants, and genotypic variation in the AhR can confer resistance to these contaminants. Thus, we characterized the AhR pathway in A. testudineus in order to understand the mechanism(s) underlying the resistance of this species to contaminants and to broaden current knowledge on teleost AhR. In A. testudineus, four AhRs, two AhR nuclear translocators (ARNTs), and one AhR repressor (AhRR) were found. Transient transfection assays revealed that AhR1a, AhR1b, and AhR2b were functional, whereas AhR2a was poorly activated by the potent agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Two ARNTs (partner of AhR) and one AhRR (repressor of AhR) all were functional with each of the active AhR. As a major form, the insensitivity of AhR2a might serve as a potential mechanism for A. testudineus’ reduced sensitivity to severe contamination. We explored the key residues that may account for AhR2a’s insensitivity in silico and then functionally validated them in vitro. Two sites (VCS322–324, M370) in its ligand-binding domain (LBD) were proved critical for its sensitivity to TCDD. This systematic exploration of the AhR pathway showed that most members have maintained their traditional functions as expected, whereas a nonfunctionalization event has occurred for AhR2a.

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Section: Introductionmentioning

confidence: 69%

Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a

Zhang

Xie

et al. 2019

Environ. Sci. Technol.

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“…In P. Lineatus , it caused genotoxic effects by accumulating and inducing changes in the tissues that interfered in antioxidant enzymes ( Palermo et al., 2015 ). Pb can interfere with the DNA repair system by inhibiting enzyme activity ( Zhang et al., 2019 ). Despite the individual effects of those elements on the genetic level, we highlight that combined metals, associated with the ambient condition, can increase toxic effects when compared to isolated chemicals ( Stankevičiūtė et al., 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Genotoxic effect of heavy metals on Astyanax lacustris in an urban stream

Silva

Benvindo‐Souza

Cotrim

et al. 2020

Heliyon

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Uncontrolled urbanization growth contributes to the pollution of aquatic environments. Heavy metals released by domestic and industrial effluents can negatively affect aquatic organisms. This study aimed to evaluate the effect of environmental pollutants, such as metals, on fish DNA damage, in stretches of an urban stream. Specimens of the Neotropical fish, Astyanax lacustris , were exposed in situ for 96 h along the Antas stream, a Brazilian aquatic system deteriorated by anthropogenic factors. Water and sediment samples were collected simultaneously for physicochemical and heavy metal analyses. The comet assay was performed as a biomarker of genotoxicity. Fish located downstream had a higher frequency of DNA damage than in the reference site. We found concentrations of Cr and Ni above acceptable levels in sediment samples. Generally, Ba, Mn, Mg, Zn, Cr, and Ni were the elements most associated with genotoxic damage. Water and sediment of the Antas stream showed genotoxic potential in A. lacustris according to the urbanization gradient, demonstrating the importance to prevent the release of environmental pollutants, especially heavy metals in urban areas.

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“…In this context, the literature suggests that the evaluation of environmental genotoxicity in different species of sh in situ generates more consistent results due to their different sensitivities to the aquatic environment (Leung et al 2014;Silva et al 2016). Zhang et al (2019) highlighted that evaluating native sh species in situ in aquatic environments not only re ects toxic impacts, but also reveal their ability to adapt to unfavorable conditions in the environment. Several anthropic activities alter land use near aquatic environments, reducing the connection between riparian vegetation cover and the aquatic environment (Gonçalves et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Environmental diagnosis of water quality in situ and ex situ in streams in Upper Paraná River Basin, Brazil

Meira

Silva

Viana

et al. 2021

Preprint

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The aim of this study was to (i) evaluate water quality using limnological parameters in three streams belonging to the Ivinhema River basin, (ii) investigate the composition and structure of the landscape around the locations sampled and their possible contribution to changes in water quality, (iii) investigate genotoxicity in fish in situ as environmental bioindicators, and (iv) evaluate the cytotoxic and genotoxic potential of water through bioassays in animal and plant models. A total of seven fish species were sampled in situ as bioindicators of environmental toxicogenetic damage: Astyanax fasciatus, Astyanax lacustris, Cyphocharax modestus, Moenkhausia forestii, Piabina argentea, Psellogrammus kennedyi, and Hypostomus ancistroides. Four types of nuclear and one cytoplasmic alteration were observed, with nuclear invagination being the most frequent. Animal bioassays showed five types of nuclear and one cytoplasmic alteration. In the plant bioassay, a reduction in germination and cell division was observed, indicating the cytotoxic and genotoxic effects of the water for Allium cepa cells. Thus, genotoxic alterations in fish and plant bioassays may reflect the different types of land use and cover around streams and the conversion of forest fragments into agricultural areas, mainly due to the expansion of sugarcane crops. The evaluation of the toxicogenetic status of the three streams is essential to emphasize the need to create projects to recover the native vegetation cover, which would improve the quality of the habitat and ensure the permanence of native species.

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The toxic effects of in situ exposure of a native fish species (Anabas testudineus) to electronic waste pollution

Cited by 23 publications

References 33 publications

Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a

Characterization of the Aryl Hydrocarbon Receptor (AhR) Pathway in Anabas testudineus and Mechanistic Exploration of the Reduced Sensitivity of AhR2a

Genotoxic effect of heavy metals on Astyanax lacustris in an urban stream

Environmental diagnosis of water quality in situ and ex situ in streams in Upper Paraná River Basin, Brazil

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