The toxicity, transport and uptake of nanoparticles in the<i>in vitro</i>BeWo b30 placental cell barrier model used within NanoTEST

Carreira, Sara Correia; Walker, Laura; Paul, Kai B.; Saunders, Margaret

doi:10.3109/17435390.2013.833317

Cited by 54 publications

(50 citation statements)

References 69 publications

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“…In lymphocytes, cytotoxicity was measured by TBE; cytotoxicity in Kupffer cells, hepatocytes and HCEC was measured using the MTT assay (Halamoda Kenzaoui et al, 2012a), in 16HBE14o by the WST-1 assay and in BeWo b30 cells by the WST-1 assay and TBE (Correia Carreira et al, 2015;Guadagnini et al, 2015b).…”

Section: Cytotoxicitymentioning

confidence: 99%

Suitability of human and mammalian cells of different origin for the assessment of genotoxicity of metal and polymeric engineered nanoparticles

et al. 2015

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Nanogenotoxicity is a crucial endpoint in safety testing of nanomaterials as it addresses potential mutagenicity, which has implications for risks of both genetic disease and carcinogenesis. Within the NanoTEST project, we investigated the genotoxic potential of well-characterised nanoparticles (NPs): titanium dioxide (TiO2) NPs of nominal size 20 nm, iron oxide (8 nm) both uncoated (U-Fe3O4) and oleic acid coated (OC-Fe3O4), rhodamine-labelled amorphous silica 25 (Fl-25 SiO2) and 50 nm (Fl-50 SiO) and polylactic glycolic acid polyethylene oxide polymeric NPs - as well as Endorem® as a negative control for detection of strand breaks and oxidised DNA lesions with the alkaline comet assay. Using primary cells and cell lines derived from blood (human lymphocytes and lymphoblastoid TK6 cells), vascular/central nervous system (human endothelial human cerebral endothelial cells), liver (rat hepatocytes and Kupffer cells), kidney (monkey Cos-1 and human HEK293 cells), lung (human bronchial 16HBE14o cells) and placenta (human BeWo b30), we were interested in which in vitro cell model is sufficient to detect positive (genotoxic) and negative (non-genotoxic) responses. All in vitro studies were harmonized, i.e. NPs from the same batch, and identical dispersion protocols (for TiO2 NPs, two dispersions were used), exposure time, concentration range, culture conditions and time-courses were used. The results from the statistical evaluation show that OC-Fe3O4 and TiO2 NPs are genotoxic in the experimental conditions used. When all NPs were included in the analysis, no differences were seen among cell lines - demonstrating the usefulness of the assay in all cells to identify genotoxic and non-genotoxic NPs. The TK6 cells, human lymphocytes, BeWo b30 and kidney cells seem to be the most reliable for detecting a dose-response.

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Section: Cytotoxicitymentioning

confidence: 99%

Suitability of human and mammalian cells of different origin for the assessment of genotoxicity of metal and polymeric engineered nanoparticles

et al. 2015

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“…7 The human, choriocarcinoma-derived BeWo cell line can be a good in vitro model system to investigate the transcellular transport of multiple nutrients and compounds. 8,9 Ali et al 10 investigated the transplacental transport of dexamethasone-loaded poly(lactic-co-glycolic acid) (PLGA) NPs using a BeWo (clone b30) cell model. The study found a tenfold increase in permeability of dexamethasone from the apical (maternal) to the basolateral (fetal) side when encapsulated within strongly anionic PLGA NPs.…”

Section: Introductionmentioning

confidence: 99%

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Tang¹,

Jiang²,

He³

et al. 2018

IJN

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IntroductionNanomedicine has shown a great potential in perinatal medicine because of its characteristics of sustained, controlled release and targeting ability; on the other hand, it may also lead to unexpected toxicities such as embryotoxicity and even malformation after crossing the placental barrier, but data concerning transplacental transport are scarce. Pullulan acetate (PA) nanoparticles (NPs) are a promising nanocarrier derived from natural polysaccharide; however, their transplacental transport ability and mechanism are unknown.Materials and methodsIn this study, fluorescein isothiocyanate (FITC) conjugated PA (PA-FITC) was synthesized. PA-FITC NPs were characterized by dynamic light scattering, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The cytotoxicity of PA-FITC NPs at concentrations of 15, 30, 60, 125, 250, 500, 1,000 and 2,000 μg/mL was studied by cell counting kit-8. The human chorionic gonadotrophin (HCG) cytokine assay was conducted to evaluate the biological function of BeWo b30 cells. Endocytic mechanisms of PA-FITC NPs were investigated via fluorescence analysis. The monolayer properties were characterized by TEM, tight junction staining, transepithelial electrical resistance and fluorescein sodium transportation. The transport ability was measured in the cell based transwell model by confocal imaging and SEM.ResultsPA-FITC NPs were almost spherical shape with a size range of 200–300 nm. Cell viability of BeWo b30 cells was up to 100% in all groups. The concentrations of HCG increased with increasing numbers of cells and culture time, which showed the good biological function of BeWo b30 cells. PA-FITC NPs were rapidly endocytosed through caveolae-mediated endocytosis and pinocytosis, with uptake inhibition rates with nystatin (NY) and colchicines (Col) of 55% and 51% respectively. BeWo b30 cell monolayer was formed over 5 days. PA-FITC NPs were found in the cytoplasm of cells on the transwell membranes; while some NPs were found in the basolateral (fetal) compartment over 24 h.ConclusionIn summary, PA-FITC NPs are nontoxic, can cross the blood-placental barrier, and show mainly internalization to BeWo b30 cells through caveolae-mediated endocytosis and pinocytosis pathways, major via the former pathway. The results could benefit the adjustment and control of the transplacental transport of nanomedicines.

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“…The BeWo cell line has been shown to be a useful in vitro placental barrier model for studying adhesion, transport, and infection [11], with many researchers using BeWo cells as an in vitro barrier model of the placenta to explore the transport and toxicity of foreign agents or organisms [12,13].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Profiling of Human Placental Trophoblasts in Response to Infection with Enterococcus faecalis

Tan

et al. 2018

Journal of Food Quality

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Increasing evidence suggests that Enterococcus faecalis strains can pass through placental barriers and cause adverse outcomes during pregnancy. However, the underlying molecular mechanism of the interaction between E. faecalis and the host placental barrier has yet to be fully elucidated. In this study, we have used DNA microarray analysis to investigate the response of human placental trophoblast-like BeWo cells to infection with E. faecalis OG1RF. These results indicate that a total of 2191 genes in BeWo cells are differentially expressed in the presence of E. faecalis OG1RF, with 1357 genes being upregulated and 834 genes being downregulated. These differentially expressed genes (DEGs) are involved in apoptosis, stress and stimulus response, placental and embryonic development, immune response, and cell adhesion. Therefore, these results provide information on the molecular mechanisms that E. faecalis invasion can trigger to cause adverse pregnancy outcomes.

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The toxicity, transport and uptake of nanoparticles in thein vitroBeWo b30 placental cell barrier model used within NanoTEST

Cited by 54 publications

References 69 publications

Suitability of human and mammalian cells of different origin for the assessment of genotoxicity of metal and polymeric engineered nanoparticles

Suitability of human and mammalian cells of different origin for the assessment of genotoxicity of metal and polymeric engineered nanoparticles

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Transcriptomic Profiling of Human Placental Trophoblasts in Response to Infection with Enterococcus faecalis

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