This study will focus on Sharia Business Units of Regional Development Banks (UUS BPD) as UUS which are considered by KNEKS to be at least ready to face separation, because of the capital factor. The separation of UUS from the parent bank based on the Banking Law no. 21 of 2008 at the latest, UUS must be carried out by the end of 2023. Using Decision Matrix Analysis through the approach of internal environmental approach (financial, human resources, operational, marketing) and external environmental approaches (political, economic, technological, socio-cultural), the results of this study aim to provide consideration for the selection of a separation model for UUS BPD. The primary data of the study came from in-depth interviews and filling out questionnaires by expert practitioners from UUS BPD who had carried out a spin-off or conversion into a sharia commercial bank, regulatory practitioners, and other expert practitioners who are judged to have knowledge/experience related to the research theme. The main considerations in the selection of the UUS BPD separation model based on the internal environmental approach are financial and operational factors, the external environmental approach is the technological factor, and the internal-external environment is the financial and operational factor. The most optimal separation model based on the internal environment, external environment, and internal-external environment is the conversion model. The next option based on the external environment and the internal-external environment is a merger, and based on the internal environment approach is a spin-off. The last option is a spin-off based on the external environment and the internal-external environment, while based on the internal environment approach is a merger.