The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction

Vasina, Daria V.; Mustafaev, O. N.; Moiseenko, Konstantin V.; Sadovskaya, N. S.; Glazunova, Olga A.; Tyurin, A. A.; Федорова, Т.В.; Павлов, А. В.; Tyazhelova, T. V.; Goldenkova-Pavlova, I. V.; Королева, О. В.

doi:10.1016/j.biochi.2015.07.015

Cited by 38 publications

(27 citation statements)

References 84 publications

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“…; Vasina et al . ). Other WRF species of the genera Lentinus and Pleurotus have also been studied because of their production of ligninolytic enzymes using agro‐industrial wastes as a sole carbon source and under solid‐state fermentation (Sarnthima et al .…”

Section: Discussionmentioning

confidence: 97%

Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source

et al. 2017

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Section: Discussionmentioning

confidence: 97%

Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source

et al. 2017

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“…similarity between detected laccases does not correlate with their origin (fungal strain). Previously, we have reported functional clusterization for laccases belonging to Trametes genus with respect to enzymes' physical, chemical, and catalytic properties (Mr, pI, carbohydrate content, and potential glycosylation sites as well as pH optimum, K m , and k cat ) [34]. The tree topology under the current study ( Fig.…”

Section: Classmentioning

confidence: 68%

“…Several studies on laccase expression showed that gene dosage is not the case [36,37]. In contrast, the difference in the physicochemical parameters and expression patterns reported previously in laccases [11,34,37] speak in favor of the subfunctionalization hypothesis. It can be hypothesized that the multigenicity of laccases should be explained by the broad spectrum of their physiological functions rather than the utilization of all of the genes in one particular process.…”

Section: Classmentioning

confidence: 81%

Laccase multigene families in Agaricomycetes

Moiseenko

Maloshenok

Vasina

et al. 2016

J. Basic Microbiol.

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Here we present the results of the exploration of laccase multigene families (MGFs) in basidiomycetous fungi from different taxonomic groups using a next generation sequencing (NGS) technology. In our study, multiple laccase genes were identified in all of the investigated fungi (13 species) from Polyporaceae, Phanerochaetaceae, Meruliaceae, Pleurotaceae, Physalacriaceae, and Peniophoraceae families. It was shown that phylogenetic positioning of the newly identified sequences exhibit patterns of clusterization with respect to enzyme properties. This can be a potentially useful tool for selecting naturally existing laccases with different physicochemical characteristics relevant to different biotechnological applications. Moreover, the method developed in this study can be used in the screening of environmental samples and fast characterization of laccase MGFs in newly identified fungal species.

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“…This suggests turning on the expression of additional isozymes, depending on accumulation of the specific inducers on different stages of lignin degradation [52]. …”

Section: Resultsmentioning

confidence: 99%

“…ostreatus lignocellulose cultures was related to impaired secretion of the protein. This can also be related to the glycolysis degree and to extracellular proteases resistance [52]. …”

Section: Resultsmentioning

confidence: 99%

Lignin-degrading peroxidases in white-rot fungus Trametes hirsuta 072. Absolute expression quantification of full multigene family

et al. 2017

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Ligninolytic heme peroxidases comprise an extensive family of enzymes, which production is characteristic for white-rot Basidiomycota. The majority of fungal heme peroxidases are encoded by multigene families that differentially express closely related proteins. Currently, there were very few attempts to characterize the complete multigene family of heme peroxidases in a single fungus. Here we are focusing on identification and characterization of peroxidase genes, which are transcribed and secreted by basidiomycete Trametes hirsuta 072, an efficient lignin degrader. The T. hirsuta genome contains 18 ligninolytic peroxidase genes encoding 9 putative lignin peroxidases (LiP), 7 putative short manganese peroxidases (MnP) and 2 putative versatile peroxidases (VP). Using ddPCR method we have quantified the absolute expression of the 18 peroxidase genes under different culture conditions and on different growth stages of basidiomycete. It was shown that only two genes (one MnP and one VP) were prevalently expressed as well as secreted into cultural broth under all conditions investigated. However their transcriptome and protein profiles differed in time depending on the effector used. The expression of other peroxidase genes revealed a significant variability, so one can propose the specific roles of these enzymes in fungal development and lifestyle.

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The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction

Cited by 38 publications

References 84 publications

Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source

Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source

Laccase multigene families in Agaricomycetes

Lignin-degrading peroxidases in white-rot fungus Trametes hirsuta 072. Absolute expression quantification of full multigene family

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