The transcardiac gradient of cardio‐microRNAs in the failing heart

Abstract

Aims Differential microRNA expression in peripheral blood has been observed in patients with heart failure, suggesting their value as potential biomarkers and likely contributors to disease mechanisms. In the present study, we aimed to evaluate the transcardiac gradient of 84 cardio‐microRNAs in healthy and failing hearts to determine which microRNAs are released or absorbed by the myocardium in heart failure. Methods and results Eight healthy volunteers and nine patients with congestive heart failure were included. Arterial and coronary sinus blood samples were collected, and microRNAs were extracted. The expression of microRNAs was analysed using real‐time PCR by the miScript miRNA PCR Array Human Cardiovascular Disease. In coronary sinus samples, the microRNAs miR‐16‐5p, miR‐27a‐3p, miR‐27b‐3p, miR‐29b‐3p, miR‐29c‐3p, miR‐30e‐5p, miR‐92a‐3p, miR‐125b‐5p, miR‐140‐5p, miR‐195‐5p, miR‐424‐5p, and miR‐451a were significantly down‐regulated, and let‐7a‐5p, let‐7c‐5p, let‐7e‐5p, miR‐23b‐3p, miR‐107, miR‐155‐5p, miR‐181a‐5p, miR‐181b‐5p and miR‐320a were up‐regulated in heart failure. Left ventricular filling pressure was negatively correlated with miR‐195, miR‐16, miR‐29b‐3p, miR‐29c‐3p, miR‐451a, and miR‐92a‐3p. The failing heart released let‐7b‐5p, let‐7c‐5p, let‐7e‐5p, miR‐122‐5p, and miR‐21‐5p, and absorbed miR‐16‐5p, miR‐17‐5p, miR‐27a‐3p, miR‐30a‐5p, miR‐30d‐5p, miR‐30e‐5p, miR‐130a‐3p, miR‐140‐5p, miR‐199a‐5p, and miR‐451a. In silico analyses suggest that the transcardiac gradient of microRNAs in heart failure may target pathways related to heart disease. Conclusion We determined the transcardiac gradient of cardio‐microRNAs in failing hearts, which supports the use of these microRNAs as potential biomarkers. The microRNAs described here may have a role in the pathophysiology of heart failure as they might be involved in pathways related to disease progression, including fibrosis.

Specific MicroRNAs comparisons in hypoxia and morphine preconditioning against hypoxia-reoxgenation injury with and without heart failure

“…20 found 3.67 fold of control group in heart failure patients [39] and after glucose fluctuations [40], it is similar in our study (3.386 fold of control), it suggests that let-7e might be an active role in the pathogenesis of heart failure, so the aberrant expression of let-7e might result in congenital heart disease.…”

Experimental, Systems, and Computational Approaches to Understanding the MicroRNA-Mediated Reparative Potential of Cardiac Progenitor Cell–Derived Exosomes From Pediatric Patients

“…PLSR analysis gave quantitative outputs to how likely each microRNA was involved in our response. Certain microRNA’s identified by our anaylsis have been shown to regulate important function like fibrosis (miR-27a and miR-29c 26, 27 ), cardiac hypertrophy (miR-29c 28 , miR-96 29, 30 , miR-182 31, 32 and miR-185 33 ), angiogenesis (miR-27a 34 ), and apoptotsis (miR-138 35, 36 , miR-25 37 ). Although, our model is predictive of microRNA-based mechanism, these targets still need to be validated in the future studies.…”

The Emerging Role of MicroRNA-155 in Cardiovascular Diseases

“…Notably, miRNA-155 was not only upregulated but also positively correlated with left ventricular mass index, a prognostic marker in HF patients. Consistent with the above results, Marques et al showed that miRNA-155 was upregulated in HF patients after screening 84 cardio-miRNAs in blood samples from 9 patients with HF and 8 healthy volunteers [40]. …”