The Transcribed 165-bp CentO Satellite Is the Major Functional Centromeric Element in the Wild Rice Species<i>Oryza punctata</i>

Zhang, Wenli; Yi, Chuandeng; Bao, Weidong; Liu, Bin; Cui, Jiajun; Yu, Hengxiu; Cao, Xiaofeng; Gu, Minghong; Liu, Min; Cheng, Zhukuan

doi:10.1104/pp.105.064147

Cited by 61 publications

(48 citation statements)

References 56 publications

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“…The map also demonstrates that the size difference between O. punctata and O. sativa is not evenly distributed over the chromosomes, possibly reflecting dynamics that have affected each chromosome differently over evolutionary time (Table 1). One possible explanation for the genome size difference is the report that O. punctata centromeres have $2.5 times more CentO satellite DNA and a wider distribution of centromeric retrotransposon of rice sequence than O. sativa (Zhang et al 2005). It warrants noting that our size estimate of the O. punctata genome may be an underestimate due to the presence of physical gaps between the contigs and the fact that the nuclear organizer region (Shishido et al 2000) on the tip of chromosome 9S was not included in the FPC map.…”

Section: Discussionmentioning

confidence: 99%

Comparative Physical Mapping Between Oryza sativa (AA Genome Type) and O. punctata (BB Genome Type)

et al. 2007

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A comparative physical map of the AA genome (Oryza sativa) and the BB genome (O. punctata) was constructed by aligning a physical map of O. punctata, deduced from 63,942 BAC end sequences (BESs) and 34,224 fingerprints, onto the O. sativa genome sequence. The level of conservation of each chromosome between the two species was determined by calculating a ratio of BES alignments. The alignment result suggests more divergence of intergenic and repeat regions in comparison to gene-rich regions. Further, this characteristic enabled localization of heterochromatic and euchromatic regions for each chromosome of both species. The alignment identified 16 locations containing expansions, contractions, inversions, and transpositions. By aligning 40% of the punctata BES on the map, 87% of the punctata FPC map covered 98% of the O. sativa genome sequence. The genome size of O. punctata was estimated to be 8% larger than that of O. sativa with individual chromosome differences of 1.5-16.5%. The sum of expansions and contractions observed in regions .500 kb were similar, suggesting that most of the contractions/ expansions contributing to the genome size difference between the two species are small, thus preserving the macro-collinearity between these species, which diverged $2 million years ago. C OMPARATIVE genome analysis is proving to be an excellent tool, not only to discover genes and understand their functions, but also to unravel the evolutionary relationships between species. Since related species are derived from recent common ancestors, it comes as no surprise that in both dicots and monocots extensive genetic collinearity was found when related species were mapped using common RFLP probe sets (Bonierbale et al. 1988;Hulbert et al. 1990;Ahn and Tanksley 1993;Jena et al. 1994). While extensive collinearity seems to be limited to the genus level among dicots (Tanksley et al.1988), sufficient collinearity exists to allow rough alignment of genetic maps across entire genomes throughout the entire cereal clade (Moore et al. 1995). The complete sequences of the model organisms Arabidopsis and rice (Arabi- Although rice is considered a model plant and placed at the center of the cereal crop syntenic circle (Moore et al. 1995; Gale and Devos 1998a,b;Devos 2005), only a few genomewide comparative analyses as yet have been performed using the rice genome sequence as a reference. The sorghum genome was compared to the rice genome sequence using two sorghum physical maps integrated with genetic markers and BAC hybridization data (Bowers et al. 2005). Various local rearrangements between other cereals and rice have been reported in sequence-level comparisons using the rice genome sequence as the reference (Chen et al. 1998;Goff et al. 2002;Bennetzen and Ma 2003;Sorrells Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. CW502583-CW509125, CW514009-539178, CW620733-CW624836, CW628185-633039, CW672722-CW676096, CW691361-692844, CW748472-CW754418, CW775494-CW77843...

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Section: Discussionmentioning

confidence: 99%

Comparative Physical Mapping Between Oryza sativa (AA Genome Type) and O. punctata (BB Genome Type)

et al. 2007

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“…5S rDNA has a single locus in the rice genome, located on the short arm of chromosome 11 [36]. In wild-type meiocytes, two unpaired 5S rDNA signals were visible at lep- Figure 5A).…”

Section: Homologous Chromosome Pairing Is Absent In Osam1-1mentioning

confidence: 99%

“…FISH analysis was conducted as described [36]. Two repetitive DNA element probes were used as the FISH probes: pTa794 had the coding sequences for the 5S rRNA genes of wheat [46] and pAtT4 contained the telomeric repeats.…”

Section: Meiotic Chromosome Preparation Fish and Immunofluorescencementioning

confidence: 99%

OsAM1 is required for leptotene-zygotene transition in rice

et al. 2011

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The events occurring at the onset of meiosis have not been fully elucidated. In the present study, OsAM1 was identified in rice (Oryza sativa L.) by map-based cloning. OsAM1, a homolog of Arabidopsis SWI1 and maize AM1, encodes a protein with a coiled-coil domain in its central region. In the Osam1 mutant, pollen mother cells are arrested at leptotene, showing that OsAM1 is required for the leptotene-zygotene transition. Immunocytological analysis revealed that OsAM1 exists as foci in early prophase I meiocytes. Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8. In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes. In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis.

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“…The anti-OsCenH3 antibody recognizes functional centromeric regions on chromosomes in rice somatic and meiotic cells (Nonomura et al, 2006). Rice 5S rDNA is located on the short arm of chromosome 11 (Zhang et al, 2005), and P0671B11 locates near the telomere of Chromosome 1. Combination of the use of these cytological markers is useful for monitoring chromosome pairing and separation.…”

Section: Mof Is Essential For Telomere Bouquet Formation and Homologomentioning

confidence: 99%

MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

Wang

Higgins

et al. 2016

Plant Cell

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The Transcribed 165-bp CentO Satellite Is the Major Functional Centromeric Element in the Wild Rice SpeciesOryza punctata

Cited by 61 publications

References 56 publications

Comparative Physical Mapping Between Oryza sativa (AA Genome Type) and O. punctata (BB Genome Type)

Comparative Physical Mapping Between Oryza sativa (AA Genome Type) and O. punctata (BB Genome Type)

OsAM1 is required for leptotene-zygotene transition in rice

MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

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