The transcription factor AtHB23 modulates starch turnover for root development and plant survival under salinity

Perotti, María Florencia; Arce, Agustín Lucas; Ariel, Federico; Figueroa, Carlos María; Chan, Raquel L.

doi:10.1016/j.envexpbot.2022.104994

Cited by 7 publications

(15 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To test the functional sense of the interaction between these three TF, we first investigated the expression patterns of AtMYB68 and AtPHL1 in roots, the organ in which AtHB23 was deeply investigated, associated with developmental and salinity responses (Perotti et al ., 2019, 2020, and 2022). For this purpose, we used 8-day-old transgenic plants carrying the constructs prAtMYB68:GUS and prAtPHL1:GUS .…”

Section: Resultsmentioning

confidence: 99%

“…Arabidopsis mutant lines phl1 -1 and −2 (SAIL_731_B09 and (SALK_079505.20.10) and DR5:GFP transgenic plants were obtained from the Arabidopsis Biological Resource Center (ABRC) stock. AtHB23 -silenced plants ( amiR23 ), prAtPHL1:GUS, prAtHB23 L :GUS, and prAtHB23 S :GUS were previously described (Perotti et al ., 2019; Spies et al ., 2022; Perotti et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

“…This gene was expressed in the lateral root primordium, acting as a negative regulator of LR initiation and in the tip of primary roots involved in the salinity response (Perotti et al, 2019). It was directly regulated by ARF7/19, and LAX3 and LBD16 are its targets (Perotti et al, 2019(Perotti et al, , 2022.…”

Section: Introductionmentioning

confidence: 99%

“…BD-HY5 + AD-STO and pDBLeu + pAD-502 were used as positive and negative controls, respectively. AtHB23ΔAHA: the C-terminal AHA domain was removed (Capella et al, 2014) AtMYB68 and AtPHL1 coexpress with AtHB23 in specific tissues and developmental stages of root development To test the functional sense of the interaction between these three TF, we first investigated the expression patterns of AtMYB68 and AtPHL1 in roots, the organ in which AtHB23 was deeply investigated, associated with developmental and salinity responses (Perotti et al, 2019(Perotti et al, , 2020(Perotti et al, , and 2022. For this purpose, we used 8-day-old transgenic plants carrying the constructs prAtMYB68:GUS and prAtPHL1:GUS.…”

mentioning

confidence: 99%

See 3 more Smart Citations

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Spies

Perotti

Cho

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Spies

Perotti

Cho

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Together HAT7, HB13, HB20 and HB23 make up the alpha clade HD-ZIP I transcription factors in Arabidopsis (Ariel et al, 2007;Henriksson et al, 2005;Mattsson et al, 1992). Genetic analysis of HAT7 has yet to be carried out, but HB13, HB20 and HB23 have been implicated in root development (Perotti et al, 2019;Silva et al, 2016), shoot development (Ribone et al, 2015), and stress responses (Ebrahimian-Motlagh et al, 2017;Gao et al, 2014;Harris et al, 2011;Perotti et al, 2021). We used CRISPR to generate hat7 loss-of-function mutants but did not observe strong phenotypes in terms of cortex cell elongation (Figure S6D-F).…”

Section: Tissue-specific Crispr Of Bri1 Confirms a Role For The Corte...mentioning

confidence: 99%

Brassinosteroid gene regulatory networks at cellular resolution

Nolan

Vukašinović

Hsu

et al. 2022

Preprint

View full text Add to dashboard Cite

Brassinosteroids (BRs) are plant steroid hormones that regulate diverse processes such as cell division and cell elongation. BRs control thousands of genes through gene regulatory networks (GRNs) that vary in space and time. We used time series single-cell RNA-sequencing to identify BR-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, uncovering the elongating cortex as a site where BRs trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HAT7 and GTL1 as BR-responsive transcription factors that regulate cell elongation in the cortex. These results establish the cortex as an important site for BR-mediated gene expression and unveil a BR signaling network regulating the transition from proliferation to elongation, illuminating new aspects of spatiotemporal hormone response.

show abstract

AtHB40 modulates primary root length and gravitropism involving CYCLINB and auxin transporters

et al. 2022

View full text Add to dashboard Cite

The transcription factor AtHB23 modulates starch turnover for root development and plant survival under salinity

Cited by 7 publications

References 72 publications

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

A complex tissue-specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Brassinosteroid gene regulatory networks at cellular resolution

AtHB40 modulates primary root length and gravitropism involving CYCLINB and auxin transporters

Contact Info

Product

Resources

About